Search results for "Inventio"
showing 10 items of 13031 documents
Taphonomic processes inconsistent with indigenous Mesolithic acculturation during the transition to the Neolithic in the Western Mediterranean
2018
Abstract We applied taphonomic analysis combined with geostatistical approaches to investigate the hypothesis that Cocina cave (Eastern Iberia) represents an acculturation context for the appearance of Neolithic Cardial pottery. In the 1970s, Fortea suggested that this important site was a prime example of acculturation because of the presence of early Neolithic pottery in late Mesolithic contexts. Since that time Cocina cave has been heralded as an example of indigenous hunter-gatherers incorporating Neolithic cultural elements into their lifeways. We analyzed the area excavated by Fortea in the 1970s by digitizing archaeological records and testing the spatial distribution of artifacts us…
Cocina cave revisited: Bayesian radiocarbon chronology for the last hunter-gatherers and first farmers in Eastern Iberia
2018
Abstract Recent excavations and radiocarbon work conducted at Cocina Cave (Valencia region, Eastern Iberia) provide new insights into the transition from foraging to farming in the eastern Iberian Peninsula between 8000 and 7300 cal yrs. BP. Cocina cave was discovered in 1940 and excavated by L. Pericot from 1941 to 1945. J. Fortea continued excavations in the 70s. Despite early international recognition and great promise of significance, the materials recovered from these excavations have only been partially analyzed and published. A new project started in 2012 is focused on these cave deposits with the main goal of understanding the occupation sequence during the neolithization process in…
Reconstruction of late Holocene autumn/winter precipitation variability in SW Romania from a high-resolution speleothem trace element record
2018
We present the first high-resolution trace element (Mg/Ca, Sr/Ca, Ba/Ca) record from a stalagmite in southwestern Romania covering the last 3.6 ka, which provides the potential for quantitative climate reconstruction. Precise age control is based on three independent dating methods, in particular for the last 250 yr, where chemical lamina counting is combined with the identification of the 20th century radiocarbon bomb peak and Th-230/U dating. Long-term cave monitoring and model simulations of drip water and speleothem elemental variability indicate that precipitation-related processes are the main drivers of speleothem Mg/Ca ratios. Calibration against instrumental climate data shows a si…
The early Upper Palaeolithic of Cova de les Cendres (Alicante, Spain)
2019
Abstract This paper presents a synthesis of the Early Upper Palaeolithic of Cova de les Cendres. Points of special attention are the sedimentary and micromorphological characterisation of level XVI, the analysis of the vegetal and animal resources and their incidence on the economy of the Gravettian human groups, and the characterisation of the landscape during this period. Furthermore, the paper offers important information of the lithic and bone assemblages, economic behaviour and radiocarbon dates of sub-levels XVIA and XVIB, related to the Gravettian, and XVIC and XVID, corresponding to the Aurignacian. Finally, the Gravettian and Aurignacian regional contexts in the Mediterranean Basin…
Late Holocene seasonal temperature variability of the western Scottish shelf (St Kilda) recorded in fossil shells of the bivalve Glycymeris glycymeris
2021
Abstract The North Atlantic Ocean and adjacent shelf seas play a crucial role in global climate. To better constrain long-term natural variability and marine-terrestrial linkages in this region, a network of highly resolved marine archives from the open ocean and continental shelves is needed. In recent decades, bivalve sclerochronology has emerged as a field providing such records from the mid- to high latitudes. In May 2014, dead valves and young live specimens of the bivalve Glycymeris glycymeris were collected at St Kilda, Scotland. A floating chronology spanning 187 years was constructed with fossil shells and radiocarbon dated to 3910–3340 cal yr before present (BP), with a probabilit…
A continuous multi-millennial record of surficial bivalve mollusk shells from the São Paulo Bight, Brazilian shelf
2014
AbstractTo evaluate the potential of using surficial shell accumulations for paleoenvironmental studies, an extensive time series of individually dated specimens of the marine infaunal bivalve mollusk Semele casali was assembled using amino acid racemization (AAR) ratios (n = 270) calibrated against radiocarbon ages (n = 32). The shells were collected from surface sediments at multiple sites across a sediment-starved shelf in the shallow sub-tropical São Paulo Bight (São Paulo State, Brazil). The resulting 14C-calibrated AAR time series, one of the largest AAR datasets compiled to date, ranges from modern to 10,307 cal yr BP, is right skewed, and represents a remarkably complete time series…
Hypothermic Stunning in Juvenile Green Turtles (Chelonia mydas) in Uruguayan Coastal Waters: Learning for Future Events
2017
Abstract Chelonia mydas in temperate areas exhibits behavioral changes for adapting to sea temperature fluctuations; however, prolonged exposure to cold temperatures can lead to hypothermia and thus hypothermic stunning events. Here we report an unusual stranding event of 90 green turtles recorded in a 12-d period in July 2012 in southeastern Uruguay, analyzing the event in an oceanographic and meteorological context. Monitoring such events provides a unique opportunity to understand the impact of hypothermic stunning on juvenile green turtle stocks that spend the entire year in this temperate region of the southwestern Atlantic Ocean.
Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine
2017
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…
Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D–cyclin-dependent kinase complexes
2017
Highlight Maize Kip-related proteins can be differentially phosphorylated by different cyclin D–cyclin-dependent kinase complexes and this influences their performance as cyclin-dependent kinase inhibitors.
A self-inducible heterologous protein expression system in Escherichia coli
2016
AbstractEscherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter’s transcriptional activity. However, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture monitoring. Here, we report the novel SILEX (Self-InducibLe Expression) system, which is a convenient, cost-effective alternative that does not require cell density monitoring or IPTG inducti…