Search results for "Investigations"

showing 10 items of 85 documents

Renin-angiotensin system inhibitors effect before and during hospitalization in COVID-19 outcomes: Final analysis of the international HOPE COVID-19 …

2021

Background The use of Renin-Angiotensin system inhibitors (RASi) in patients with coronavirus disease 2019 (COVID-19) has been questioned because both share a target receptor site. Methods HOPE-COVID-19 (NCT04334291) is an international investigator-initiated registry. Patients are eligible when discharged after an in-hospital stay with COVID-19, dead or alive. Here, we analyze the impact of previous and continued in-hospital treatment with RASi in all-cause mortality and the development of in-stay complications. Results We included 6503 patients, over 18 years, from Spain and Italy with data on their RASi status. Of those, 36.8% were receiving any RASi before admission. RASi patients were …

QA75Malemedicine.medical_specialtyHeart diseaseInfecciones por coronavirusmedicine.medical_treatmentInhibidores enzimáticosPopulationClinical InvestigationsEnfermedad transmisibleAngiotensin-Converting Enzyme InhibitorsSistema renina-angiotensinaComorbidity030204 cardiovascular system & hematologySeverity of Illness Index03 medical and health sciences0302 clinical medicineRisk FactorsOutcome Assessment Health CareSeverity of illnessmedicineHumansRegistries030212 general & internal medicineMedical prescriptioneducationHeart FailureMechanical ventilationeducation.field_of_studySARS-CoV-2business.industryCOVID-19Middle AgedPrognosismedicine.diseaseR1Respiration ArtificialComorbidityHospitalizationItalySpainHeart failureEmergency medicineFemaleObservational studyCardiology and Cardiovascular MedicinebusinessAmerican Heart Journal
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Functional Connection Between the Clb5 Cyclin, the Protein Kinase C Pathway and the Swi4 Transcription Factor in Saccharomyces cerevisiae

2005

Abstract The rsf12 mutation was isolated in a synthetic lethal screen for genes functionally interacting with Swi4. RSF12 is CLB5. The clb5 swi4 mutant cells arrest at G2/M due to the activation of the DNA-damage checkpoint. Defects in DNA integrity was confirmed by the increased rates of chromosome loss and mitotic recombination. Other results suggest the presence of additional defects related to morphogenesis. Interestingly, genes of the PKC pathway rescue the growth defect of clb5 swi4, and pkc1 and slt2 mutations are synthetic lethal with clb5, pointing to a connection between Clb5, the PKC pathway, and Swi4. Different observations suggest that like Clb5, the PKC pathway and Swi4 are in…

Saccharomyces cerevisiae ProteinsMitotic crossoverBlotting WesternMutantSaccharomyces cerevisiaeSaccharomyces cerevisiaeInvestigationsCyclin BBiologymedicine.disease_causeGeneticsmedicineHydroxyureaImmunoprecipitationDNA FungalFluorescent Antibody Technique IndirectTranscription factorProtein Kinase CProtein kinase CCyclinRecombination GeneticGeneticsMutationKinaseCell CyclefungiFlow Cytometrybiology.organism_classificationMolecular biologyCell biologyDNA-Binding ProteinsMutationChromosomes FungalTranscription FactorsGenetics
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Functional distinction between Cln1p and Cln2p cyclins in the control of the Saccharomyces cerevisiae mitotic cycle.

2004

Abstract Cln1p and Cln2p are considered as equivalent cyclins on the basis of sequence homology, regulation, and functional studies. Here we describe a functional distinction between the Cln1p and Cln2p cyclins in the control of the G1/S transition. Inactivation of CLN2, but not of CLN1, leads to a larger-than-normal cell size, whereas overexpression of CLN2, but not of CLN1, results in smaller-than-normal cells. Furthermore, mild ectopic expression of CLN2, but not of CLN1, suppresses the lethality of swi4swi6 and cdc28 mutant strains. In the absence of Cln1p, the kinetics of budding, initiation of DNA replication, and activation of the Start-transcription program are not affected; by cont…

Saccharomyces cerevisiae ProteinsMutantSaccharomyces cerevisiaeBlotting WesternMitosisSaccharomyces cerevisiaeBiologyInvestigationsmedicine.disease_causeS PhaseCyclinsGeneticsmedicineImmunoprecipitationFluorescent Antibody Technique IndirectMitosisCyclinCell SizeGeneticsCyclin-dependent kinase 1MutationDNA replicationbiology.organism_classificationBlotting NorthernBridged Bicyclo Compounds HeterocyclicFlow CytometryMolecular biologyThiazolesMutationThiazolidinesEctopic expressionGenetics
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Specific Defects in Different Transcription Complexes Compensate for the Requirement of the Negative Cofactor 2 Repressor in Saccharomyces cerevisiae

2007

Abstract Negative cofactor 2 (NC2) has been described as an essential and evolutionarily conserved transcriptional repressor, although in vitro and in vivo experiments suggest that it can function as both a positive and a negative effector of transcription. NC2 operates by interacting with the core promoter and components of the basal transcription machinery, like the TATA-binding protein (TBP). In this work, we have isolated mutants that suppress the growth defect caused by the depletion of NC2. We have identified mutations affecting components of three different complexes involved in the control of basal transcription: the mediator, TFIIH, and RNA pol II itself. Mutations in RNA pol II in…

Saccharomyces cerevisiae ProteinsTranscription GeneticRepressorRNA polymerase IISaccharomyces cerevisiaeInvestigationsGeneticsPromoter Regions GeneticTranscription factorAllelesGeneticsAdenosine TriphosphatasesTATA-Binding Protein Associated FactorsbiologyGeneral transcription factorDNA HelicasesPromoterPhosphoproteinsRepressor ProteinsProtein SubunitsTranscription Factor TFIIHMutationTranscription factor II Hbiology.proteinTrans-ActivatorsTranscription Factor TFIIBMutant ProteinsTranscription Factor TFIIDRNA Polymerase IITranscription factor II BTranscription Factor TFIIHTranscription Factors
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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The principle of mandatory criminal prosecution and the independence of public prosecutors in the Italian criminal justice system

2010

The purpose of this article is to explain the reasons for adopting, in Italy, the principle of mandatory criminal prosecution. This rule is closely connected to the role and functions of Italian public prosecutors. The supreme guarantee deriving from the rule governing the mandatory status of criminal prosecution is to safeguard the equal treatment of all citizens before criminal law. This is possible only if criminal prosecution is the responsibility of a public body carrying out public functions without any external interference. This is the reason for the independence of public prosecutors from the other powers of the state. But, there has been a tendency to separate the status of public…

Settore IUS/16 - Diritto Processuale Penalepublic prosecutor independence ptinciple of mandatory criminal prosecution investigations impartiality-objectivity
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Ancora sulle "esplosive palatali" nelle inchieste dell'Atlante Linguistico del Mediterraneo (ALM): i punti albanesi

2021

Tra i «Problemi redazionali» emersi in fase di controllo e organizzazione dei materiali giunti dalla campagna di rilevamenti dell’Atlante Linguistico del Mediterraneo (ALM), Berruto (1971-1973) rileva la proliferazione e l’eterogeneità di alcune scelte grafemiche operate da diversi raccoglitori che, impegnati in una trascrizione più o meno puntuale delle risposte ottenute, avevano interpretato e risolto variamente alcune delle indicazioni previste nel sistema di trascrizione fonetica che accompagnava il Questionario. Particolarmente complesso era emerso il quadro relativo alla trascrizione di alcune consonanti del settore “palatale”, per le quali i grafemi (e i foni a essi corrispondenti) p…

Settore L-FIL-LET/12 - Linguistica ItalianaAmong the «Editorial problems» that emerged during the control and organization of the materials coming from the survey campaign of the Atlante Linguistico del Mediterraneo (ALM) Berruto (1971-1973) notes the proliferation and heterogeneity of some graphemic choices made by different field researchers. As they were engaged in a more or less punctual transcription of the answers obtained they had interpreted and solved differently some of the directions provided in the phonetic transcription system that accompanied the Questionnaire. The picture relating to the transcription of some consonants of the palatal sector had emerged as particularly complex since the graphemes (and the corresponding phones) foreseen in the original system (ALM 1959) were insufficient. Not even the 1971 updates meant to improve the representation of some phonetic features emerged from the investigations in the operations of Response reviewing allowed to obtain a clear homogeneous picture regarding the «plosive palatal» consonants. With regard to the phonetic trait under scrutiny I have already had the opportunity to propose a path aiming to the achievement of possible operational solutions. This has taken place in view of the planned simplification / homologation and transcoding phase from the ALM original system to the IPA one - while proceeding from the examination of the Schedoni’s current state with regard to the surveys referring to central-southern Italy Corsica and Sicily (see Matranga 2019). The scope is now to investigate the same phonetic trait as proven in the materials about the surveys carried out by Lirak Dodbiba in the ALM’s three Albanian points.
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Malformations of central nervous system: General issues

2013

Malformations of the central nervous system (CNS) encompass a heterogeneous group of congenital anomalies that may be isolated or appear as part of a genetic syndrome. Advances in identifying the genetic etiology underlying many CNS malformation and syndromes have led to the current genetic-based classifications that allows us to better estimate prognosis and potential complications. Herein, we discuss the main genetic, clinical and radiological features and their implications for diagnostic testing and disease management

Settore MED/38 - Pediatria Generale E SpecialisticaSettore MED/31 - OtorinolaringoiatriaCNS malformations genetic investigations developmental delay.
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La Valle dei Templi in epoca medioevale. Caratterizzazione antropologica e paleopatologica delle sepolture antistanti in Tempio della Concordia

2021

Riassunto ― Il lavoro presenta i risultati delle analisi bio-archeologiche effettuate su resti scheletrici umani rinvenuti in quattordici sepolture di epoca medioevale rinvenute nel Parco Archeologico della Valle dei Templi di Agrigento (Sicilia). L’obiettivo è stato l’acquisizione delle informazioni necessarie per la ricostruzione del profilo biologico di ciascun individuo, al fine di determinarne il sesso, la stima dell’età biologica alla morte, la stima della statura e la valutazione delle patologie e degli indicatori di stress occupazionale mediante le correnti metodologie e tecniche diagnostiche di tipo antropologico. Sebbene il cattivo stato di conservazione di alcuni individui non ne…

Summary ― The work presents the results of the bio-archaeological analyzes carried out on human skeletal remains found in fourteen burials of Medieval chronology at the Archaeological Park of Valle dei Templi in Agrigentum (Sicily). The aim of the work was the acquisition of the information necessary for the reconstruction of the biological profile of each individual throught the determination of sex the estimate of the biological age at death the estimate of height and the evaluation of pathologies and markers of occupational stress through current anthropological diagnostic methodologies and techniques. Althought the poor state of conservation of some individuals has not allowed their whole anthropological characterization the investigations have highlighted the heterogeneity relating to the age groups and have allowed us to ascertain the presence of pathological alterations in subjects of mature age sometimes of unknown etiology such as DISH (Diffuse Idiopathic Hyperostosis). The sample size is not representative of the entire population but subsequent insights will provide a better understanding of the population dynamics of Medieval Agrigentum.Settore BIO/08 - Antropologia
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Application of Thermographic Techniques for the Detection of Failures on Photovoltaic Modules

2019

The paper focuses on thermography as a useful investigation tool for diagnosis, detection and prevention of failures of photovoltaic modules. The paper describes the faults detectable by thermographic investigations and how to conduct these investigantions. Then the results of some analyses conducted on a photovoltaic field are presented. The investigations made possible to identify faulty modules with hot spots by measuring localized superheat due to the presence of concentrated deposits on the exposed surface of the module and the superheating due to the presence of sand/dust deposits distributed on a significant portion of the module surface. The thermographic investigations can be used …

SuperheatingSettore ING-IND/33 - Sistemi Elettrici Per L'EnergiaComputer sciencefaults PV modules thermographic investigations020209 energy020208 electrical & electronic engineeringThermographyPhotovoltaic system0202 electrical engineering electronic engineering information engineeringSettore ING-IND/10 - Fisica Tecnica Industriale02 engineering and technologySettore ING-IND/32 - Convertitori Macchine E Azionamenti ElettriciAutomotive engineering
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