Search results for "Iri"
showing 10 items of 12514 documents
Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)
2008
BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…
Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection
2019
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
Antiviral prophylaxis in patients with haematological malignancies and solid tumours: Guidelines of the Infectious Diseases Working Party (AGIHO) of …
2006
Morbidity and mortality in patients with malignancies are increased by viral infections. These mostly are reactivations of asymptomatic latent infections. They primarily concern clinical entities associated with the reactivation of herpes viruses, such as varicella zoster virus (VZV) and cytomegalovirus (CMV). Respiratory tract infections caused by influenza, parainfluenza or respiratory syncytial virus (RSV) are less common. Since reactivation of latent infections has major clinical impact, antiviral prophylaxis is an attractive approach for patients expecting immunosuppression. The main risk factor for clinically relevant reactivation is profound disruption of cellular immune response. Du…
Enhancement by TNF-alpha of reactivation and replication of latent herpes simplex virus from trigeminal ganglia of mice.
1995
The influence of tumor-necrosis-factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukine-1 (IL-1) and IL-3 on the in vitro reactivation frequency and replication rate of trigeminal ganglia of mice latently infected with herpes simplex virus (HSV) strain KOS was studied. It could be demonstrated that TNF-alpha and possibility GM-CSF, but not IL-1 and IL-3, enhanced the reactivation frequency and replication of HSV. Interferon alpha/beta (IFN alpha/beta) prevented reactivation and replication.
A single tube PCR assay for detecting viruses and their recombinants that cause tomato yellow leaf curl disease in Mediterranean basin
2007
Tomato yellow leaf curl disease (TYLCD) is well known in Mediterranean countries, where it has been causing severe losses in tomato crops for decades. Until recently, two viruses (with several isolates) in the genus Begomovirus, family Geminiviridae, have been associated with the epidemics: Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV). However, recombinants between these, such as Tomato yellow leaf curl Malaga virus (TYLCMalV), are spreading, and new methods for detecting all viruses present in the region are needed. By considering all DNA sequences available of viruses causing TYLCD in the Mediterranean basin, a PCR/RFLP protocol was developed t…
Caracterización del viroma en plasma de donantes de sangre
2022
El desarrollo de la metagenómica ha impulsado el descubrimiento de un número notable de nuevas secuencias virales, lo que ha permitido conocer la composición de las comunidades virales de múltiples ambientes, entre los que se incluye el cuerpo humano. El viroma humano se caracteriza por presentar una enorme heterogeneidad, en cada localización corporal existe un ambiente determinado, lo que se refleja en una composición muy diversa de las comunidades de virus y bacterias presentes. En concreto, el plasma sanguíneo se caracteriza por albergar diferentes virus, incluso en individuos aparentemente sanos, como los donantes de sangre. Para caracterizar los virus presentes en muestras de plasma, …
Il ravvicinamento delle legislazioni in materia di visti, asilo, immigrazione
2013
Il saggio è stato promosso nel contesto delle attività di ricerca connesse con il Prin 2008 - Unità dell'Università di Palermo di cui il prof. Verde è responsabile locale
Il difficile bilanciamento tra tutela della madre vulnerabile, best interest del minore e diritti culturali della donna migrante al vaglio della Cort…
2021
Il contributo offre il commento di una recente della Corte Europea dei Diritti dell’Uomo riguardante un caso di violazione del diritto alla vita privata e familiare, di cui all’art. 8 della Convenzione Europea dei Diritti dell’Uomo, da parte della Repubblica Italiana. L’autrice ricostruisce i principali fatti che caratterizzano il caso concreto e le questioni di diritto sottoposte all’attenzione della Corte, analizzando la sentenza in commento sulla base di due essenziali nodi logico-argomenta- tivi: il primo riguarda il difficile contemperamento tra i valori protetti dalla normativa in materia di adozione e il diritto di genitori e figli a mantenere in vita, per quanto compatibile con il b…