Search results for "Isolation"

P2.07: Evaluation of Different Exosomal RNA Isolation Methods in NSCLC Liquid Biopsies: Track: Biology and Pathogenesis

2016

Isolation of T Cells from the Gut

2014

The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the intestinal mucosa like regulating intestinal homeostasis, maintaining epithelial barrier function as well as regulating adaptive and innate immune responses. To analyze the role of these cells in different disease states, it is necessary to isolate pure cell populations of the intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) of the gut. In this protocol we describe a method to isolate T cells from IEL and LPL, which can be used for further investigations like comparative studies of mRNA expression, cell …

Housing conditions modulate the reinforcing properties of cocaine in adolescent mice that binge on fat

2017

Abstract Binge eating is a specific form of overeating characterized by intermittent, excessive eating. To date, several studies have addressed the effects that bingeing on fat has on the rewarding effects of drugs of abuse, but they have found contradictory and highly variable results. Housing conditions could modulate these results, as most studies employ isolated animals to measure the exact amount of food that is ingested. The aim of this study was to evaluate the effects of housing conditions on the response of mice to cocaine, modulated by bingeing on a high-fat diet during adolescence. After 40 days of binge-eating for 2 h, three days a week (PND 29–69), the reinforcing effects of a …

Fault detection for discrete-time Markov jump linear systems with partially known transition probabilities

2010

In this article, the fault detection (FD) problem for a class of discrete-time Markov jump linear system (MJLS) with partially known transition probabilities is investigated. The proposed systems are more general, which relax the traditional assumption in Markov jump systems that all the transition probabilities must be completely known. A residual generator is constructed and the corresponding FD is formulated as an H ∞ filtering problem by which the error between residual and fault are minimised in the H ∞ sense. The linear matrix inequality-based sufficient conditions for the existence of FD filter are derived. A numerical example on a multiplier–accelerator model economic system is give…

Not a footprint to be seen: isolation in the interplay between words, music and image in two Dutch-language dubbed versions of ‘Let it go’

2019

Animated musical films, such as Disney’s Frozen, have long been immensely popular among the globe; however, the investigation of the songs of such films in a dubbing context constitutes a relatively new area of research. This paper compares the theme of isolation in one song from Frozen, ‘Let it go’, in the two Dutch-language dubbed versions released in, respectively, the Netherlands and Belgium. The triangle of aspects, an analytical model for animated musical films, is used to analyse the musical, visual, and verbal codes of ‘Let it go’ and categorise differences between the TTs and the ST. This model combines qualitative and quantitative data to compare different-language versions of son…

Auf den Spuren öffentlicher Meinung im mittelhochdeutschen Minnesang

2011

The scope of this study is to analyse if and how traces of public opinion, in the sociopsychological sense of social control, may be found in the German medieval love lyrics. With the aid of a hermeneutic text analysis some selected Middle High German minnesongs are exemplarily examined. The analysis shows that awareness of public opinion as social control did exist in the Middle Ages. On the one hand, there are minnesongs that raise the topics ere (honour) and spot (mockery). In these songs public mockery represents a threat of isolation against individuals who do not conform to public opinion. Because mockery may lead to loss of honour and to social isolation, mockery pressures individual…

Identification and characterization of a novel Ets-2-related nuclear complex implicated in the activation of the human interleukin-12 p40 gene promot…

1997

Interleukin-12 (IL-12) is a proinflammatory cytokine produced by antigen-presenting cells in response to many microbial infections. IL-12 plays an important role in the generation of T helper type-1 cells, which favor cell-mediated immune response. IL-12 is composed of two different subunits, p40 and p35, whose expression can be regulated concomitantly or differentially. Monocytic cells, the major producers of IL-12, can be primed by interferon-gamma (IFN-gamma) to produce optimal amounts of IL-12 in response to LPS stimulation as a consequence of bacterial infection. The priming effect is exerted primarily at the transcriptional level on the p40 promoter in conjunction with the effects of …

Social strategies and loneliness: A prospective study

1997

Abstract The aim of this longitudinal study was to examine whether the feeling of loneliness is rather an antecedent or a consequence of the strategies young adults apply in social situations. To investigate this, university students were asked to fill in the Strategy and Attribution Questionnaire at the beginning of their first and third years at university, and the revised UCLA Loneliness scale at the beginning of their second and fourth years. The results showed that the more the young adults reported the use of a pessimistic-avoidance strategy, the less lonely they were later on. In turn, the more lonely the students were, the less they used an approach-oriented strategy later on. Final…

Effects of pre-operative isolation on postoperative pulmonary complications after elective surgery: an international prospective cohort study

2021

We aimed to determine the impact of pre-operative isolation on postoperative pulmonary complications after elective surgery during the global SARS-CoV-2 pandemic. We performed an international prospective cohort study including patients undergoing elective surgery in October 2020. Isolation was defined as the period before surgery during which patients did not leave their house or receive visitors from outside their household. The primary outcome was postoperative pulmonary complications, adjusted in multivariable models for measured confounders. Pre-defined sub-group analyses were performed for the primary outcome. A total of 96,454 patients from 114 countries were included and overall, 26…

An LMI approach to vibration control of base-isolated building structures with delayed measurements

2010

In this article, we address a convex optimisation approach to the problem of state-feedback H∞ control design for vibration reduction of base-isolated building structures with delayed measurements, where the delays are time-varying and bounded. An appropriate Lyapunov-Krasovskii functional and some free-weighting matrices are utilised to establish some delay-range-dependent sufficient conditions for the design of desired controllers in terms of linear matrix inequalities. The controller, which guarantees asymptotic stability and an H∞ performance, simultaneously, for the closed-loop system of the structure, is then developed. The performance of the controller is evaluated by means of simula…