Search results for "KLF4"

showing 6 items of 16 documents

Abstract 3056: Lung tumor spheres as in vitro platform for testing new therapeutic strategies against cancer stem cells

2018

Abstract Background: Treatment resistance is related to cancer stem cells (CSCs), a highly tumorigenic subpopulation of cells capable of growing and forming tumor spheres under non-adherent conditions. This study aimed to isolate and characterise CSCs from resected non-small cell lung cancer (NSCLC) patients' tumor-tissue and cell lines like tumor spheres and to use them as an in vitro platform for drug screening. Methods: The study was performed on tumour cells from 8 resected NSCLC patients and 12 NSCLC cell lines grown in monolayer and as spheres. The expression of 60 genes, including CSC-markers, pluripotency inducers, cell cycle regulators and components of the Notch, Wnt and Hedgehog …

Homeobox protein NANOGCancer ResearchOncologybiologySOX2KLF4Cancer stem cellCellular differentiationCD44Cancer researchbiology.proteinCytotoxic T cellCD90Cancer Research
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Abstract 3354: Characterization of lung tumorspheres by gene expression and flow cytometry: differential expression in CSC-related markers and signal…

2016

Abstract Chemoresistance, progression and metastasis have made of lung cancer the first cause of cancer mortality. These features were linked to a subpopulation of cells, named cancer stem cells (CSCs), which remain largely unknown. The aim of this study was to isolate and characterize CSCs from lung cancer cell-lines and tumor-tissue from resectable non-small cell lung cancer (NSCLC). Methods: Tumor cells from resected NSCLC and cell lines (H1650, H1993, A549, and PC9) were grown in monolayer and as spheroids. RTqPCR was performed to analyze the mRNA expression of CSCs-related genes: CSC-markers (EPCAM1, ALDH1A1, CD166, ABCG2, CD44, CD133); pluripotency genes (KLF4, OCT4, NANOG, SOX2, MYC,…

Homeobox protein NANOGCancer ResearchbiologyCD44Wnt signaling pathwayCancerStem cell markermedicine.diseaseOncologySOX2KLF4Cancer stem cellembryonic structuresbiology.proteinCancer researchmedicineCancer Research
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Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune Evasion

2015

mRNA reprogramming results in the generation of genetically stable induced pluripotent stem (iPS) cells while avoiding the risks of genomic integration. Previously published mRNA reprogramming protocols have proven to be inconsistent and time-consuming and mainly restricted to fibroblasts, thereby demonstrating the need for a simple but reproducible protocol applicable to various cell types. So far there have been no published reports using mRNA to reprogram any cell type derived from human blood. Nonmodified synthetic mRNAs are immunogenic and activate cellular defense mechanisms, which can lead to cell death and inhibit mRNA translation upon repetitive transfection. Hence, to overcome RNA…

Homeobox protein NANOGCellular Reprogramming TechniquesInduced Pluripotent Stem CellsVaccinia virusFibroblastsBiologyTransfectionLIN28Molecular biologyCell biologyKruppel-Like Factor 4MicroRNAsSOX2KLF4GeneticsHumansMolecular MedicineCellular Reprogramming TechniquesRNA MessengerProgenitor cellInduced pluripotent stem cellMolecular BiologyReprogrammingEndothelial Progenitor CellsImmune EvasionHuman Gene Therapy
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Gata4 Blocks Somatic Cell Reprogramming By Directly Repressing Nanog

2012

Abstract Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by ectopic expression of the four factors Oct4, Klf4, Sox2, and Myc. Here, we investigated the role of Gata4 in the reprogramming process and present evidence for a negative role of this family of transcription factors in the induction of pluripotency. Coexpression of Gata4 with Oct4, Klf4, and Sox2 with or without Myc in mouse embryonic fibroblasts greatly impaired reprogramming and endogenous Nanog expression. The lack of Nanog upregulation was associated with a blockade in the transition from the initiation phase of reprogramming to the full pluripotent state characteristic of iPS cells. Addition of Nanog …

Pluripotent Stem CellsTranscriptional ActivationHomeobox protein NANOGChromatin ImmunoprecipitationTranscription GeneticRex1Kruppel-Like Transcription FactorsDown-RegulationElectrophoretic Mobility Shift AssayBiologyCell LineProto-Oncogene Proteins c-mycKruppel-Like Factor 4MiceSOX2AnimalsRNA MessengerRNA Small InterferingInduced pluripotent stem cellEmbryonic Stem Cellsreproductive and urinary physiologyHomeodomain ProteinsSOXB1 Transcription FactorsNanog Homeobox ProteinCell DifferentiationNanog Homeobox ProteinCell BiologyCellular ReprogrammingEmbryonic stem cellGATA4 Transcription FactorKLF4embryonic structuresHepatocyte Nuclear Factor 3-betaCancer researchMolecular MedicineRNA Interferencebiological phenomena cell phenomena and immunityOctamer Transcription Factor-3ReprogrammingDevelopmental BiologyStem Cells
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The importance of culturing primary cells under physiological conditions: proliferation, senescence, pluripotency

2017

Mesenchymal stem cells (MSCs), such as human dental pulp stem cells (hDPSCs), are currently a source for cell therapy. However, cell therapy protocols require 10–400 million cells per treatment, and consequently, they need to be expanded in vitro before implantation, with the inconvenience that MSCs undergo senescence following a certain number of cell expansion passages, loosing their stem cell qualities. Ambient oxygen tension (21% pO2) is normally used for in vitro culture, but physiological levels in vivo range between 3% and 6% pO2. We previously demonstrated that hDPSC proliferation rate is significantly lowered at 21% pO2 due to enhanced oxidative stress, which led to the activation …

SenescenceMesenchymal stem cellBiologymedicine.disease_causeBiochemistryCell biologyCell therapySOX2KLF4Physiology (medical)Dental pulp stem cellsmedicineStem cellOxidative stressFree Radical Biology and Medicine
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Analysis of the molecular mechanisms that mediate the therapeutic actions of glucocorticoids in skin

2017

Tesis doctoral 189 páginas, 53 figuras, 1 tabla como material suplementario

skinPiel -- Enfermedades -- FarmacoterapiaUNESCO::CIENCIAS DE LA VIDAglucocorticoid receptorGlucocorticoides -- Uso terapeúticokeratinocytegilzzfp36klf4mouse:CIENCIAS DE LA VIDA [UNESCO]
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