Search results for "Kd"
showing 10 items of 540 documents
Cellular Prion Protein Participates in Amyloid-β Transcytosis across the Blood—Brain Barrier
2012
The blood—brain barrier (BBB) facilitates amyloid-β (Aβ) exchange between the blood and the brain. Here, we found that the cellular prion protein (PrPc), a putative receptor implicated in mediating Aβ neurotoxicity in Alzheimer's disease (AD), participates in Aβ transcytosis across the BBB. Using an in vitro BBB model, [125I]-Aβ1–40 transcytosis was reduced by genetic knockout of PrPc or after addition of a competing PrPc-specific antibody. Furthermore, we provide evidence that PrPc is expressed in endothelial cells and, that monomeric Aβ1–40 binds to PrPc. These observations provide new mechanistic insights into the role of PrPc in AD.
Detection of fluorine using laser-induced breakdown spectroscopy and Raman spectroscopy
2017
In general, the detection of F and other halogens is challenging through conventional techniques. In this paper, various approaches for the qualitative and quantitative analysis of F using the laser-induced breakdown spectroscopy (LIBS) technique were demonstrated. In LIBS, fluorine detection can be realized by means of atomic lines and molecular bands. For the purposes of our experiment, two sets of pellets with various contents of CaF2, CaCO3 and cellulose were analyzed using a lab-based LIBS system under a He atmosphere. The fluorine atomic line at 685.60 nm was correlated with CaF signals proving their close relationship. Consequently, the limits of detection were determined for both an…
The role of ICOS in directing T cell responses: ICOS-dependent induction of T cell anergy by tolerogenic dendritic cells.
2009
Abstract Tolerogenic dendritic cells (DC) play an important role in maintaining peripheral T cell tolerance in steady-state conditions through induction of anergic, IL-10-producing T cells with suppressive properties. ICOS, an activation-induced member of the CD28 family on T cells, is involved in the induction of IL-10, which itself could contribute to induction of anergy and development of suppressive T cells. Therefore, we analyzed the functional role of ICOS in the differentiation process of human CD4+ T cells upon their interaction with tolerogenic DC. We compared the functional properties of CD4+ T cells from healthy volunteers and ICOS-deficient patients after stimulation with tolero…
The Fumarate/Succinate Antiporter DcuB of Escherichia coli Is a Bifunctional Protein with Sites for Regulation of DcuS-dependent Gene Expression
2008
DcuB of Escherichia coli catalyzes C4-dicarboxylate/succinate antiport during growth by fumarate respiration. The expression of genes of fumarate respiration, including the genes for DcuB (dcuB) and fumarate reductase (frdABCD) is transcriptionally activated by C4-dicarboxylates via the DcuS-DcuR two-component system, comprising the sensor kinase DcuS, which contains a periplasmic sensing domain for C4-dicarboxylates. Deletion or inactivation of dcuB caused constitutive expression of DcuS-regulated genes in the absence of C4-dicarboxylates. The effect was specific for DcuB and not observed after inactivation of the homologous DcuA or the more distantly related DcuC transporter. Random and s…
Drošības radīšanas mehānismi jauniešu ģimenēs
2015
Bakalaura darbs “Drošības radīšanas mehānismi jauniešu ģimenēs” ir pētījums par jauniešu ģimenēm, kur viens vai abi vecāki ir 18-25 gadu vecumā un audzina vienu vai vairākus bērnus. Tā mērķis ir noskaidrot, kā jauno vecāku ģimenes pašas vērtē savu drošumspēju un kādi drošības radīšanas mehānismi tajās tiek izmantoti. Balstoties uz cilvēkdrošības perspektīvu, pētījuma laikā tika veiktas intervijas ar jauniešiem, lai atbildētu uz pētnieciskajiem jautājumiem, kā jaunie vecāki skaidro labu un jēgpilnu dzīvi, kādi ir jauno vecāku mehānismi, lai sasniegtu vēlamo drošību un kā jaunie vecāki vērtē savas iespējas radīt vēlamos dzīves apstākļus. Iegūtie pētījuma rezultāti atklāj, ka iespēja radīt nea…
Vide mijiedarbē ar cilvēku: Ogres iedzīvotāju fleksibilitāte ārkārtas situācijās plūdu laikā
2017
Šajā bakalaura darbā pētīta Ogres iedzīvotāju spēja pielāgoties cilvēknedrošām situācijām. Pētījums apskata Ogres iedzīvotāju fleksibilitāti plūdu laikā. Darba mērķis ir noskaidrot šīs pilsētas iedzīvotāju informētību un izpratni par cilvēkdrošu vidi un identificēt Ogres iedzīvotāju pielāgošanās stratēģijas ārkārtas situācijās. Pētījuma ietvaros tika aptaujāti seši Ogres novada Ogresgala ciema apdzīvotās vietas “Dārziņi” iedzīvotāji, lai uzzinātu, kādēļ viņi izvēlas dzīvot vietā, kas pakļauj viņu drošību riskam, kā viņu lēmums atspoguļo izpratni un informētību par cilvēkdrošu vidi un kā aptaujātie cilvēki pielāgojušies dzīvei šādā vidē. Pētījuma rezultāti liecina, ka Ogres iedzīvotāji ir in…
Rīgas bezpajumtnieku apmetņu materiālā ekoloģija
2015
Maģistra darbs aplūko veidus, kā Rīgas bezpajumtnieki pilsētas vidē veido savu personisko telpu apmetņu formā. Pētījumā lietotas etnogrāfiskās metodes, kā līdzdalīgais novērojums, līdzdošanās un padziļinātās intervijas, lai pievērstos dažādām apmetņu materiālo apstākļu dimensijām bezpajumtnieku ikdienas dzīvē. Pētījuma centrālais arguments atklāj, ka apmetnes veido paplašinātu ķermenisko telpu, kas ne tik daudz raksturo personu, bet plašākus sabiedrības sociāli ekoloģiskos procesus. Apmetnes rada to apdzīvošana caur savu un sevis izmantoto materiālu klātbūtni. Apmetnes veido materiālu un ķermeņu plūsmas mezglu punktus, kuriem ir laika, telpas, kustības, iemiesotības, afektīvā un legālā dime…
Knock-down of the oxysterol receptor LXRα impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRβ activation.
2012
Liver X Receptors (LXRs) α and β are oxysterol-activated nuclear receptors involved in the control of lipid metabolism and inflammation. Pharmacological activation of LXR is promising in the treatment of atherosclerosis since it can promote cholesterol efflux from macrophages and prevent foam cell formation. However, the development of LXR agonists has been limited by undesirable side-effects such as hepatic steatosis mediated by LXRα activation. Therefore, it has been proposed that targeting LXRα activators to extrahepatic tissues or using LXRβ-specific activators could be used as alternative strategies. It is not clear whether these molecules will retain the full atheroprotective potentia…
Wronskian Addition Formula and Darboux-Pöschl-Teller Potentials
2013
For the famous Darboux-Pöschl-Teller equation, we present new wronskian representation both for the potential and the related eigenfunctions. The simplest application of this new formula is the explicit description of dynamics of the DPT potentials and the action of the KdV hierarchy. The key point of the proof is some evaluation formulas for special wronskian determinant.
Relation of oxidant stress with endothelial activation in hemodialysis.
2006
Introduction and Aims: Enhanced oxidative stress may be implicated in the pathogenesis of atherosclerosis in hemodialysed patients (HDs). We hypothesized that in hypertensive HDs oxidative stress should be elevated and associated to endothelial activation, and inflammation. Methods:We examined oxidative stress, as measured by 8-ISO-PGF2alpha, endothelial activation by measuring endothelin-1 (ET-1) and adhesion molecules ICAM-1 and VCAM-1, and the biomarker of acute-phase inflammation CRP. In 52 mild HDs and in 50 healthy controls (Cs) we analysed, in the interdialytic day, plasma levels of 8-ISO-PGF2alpha, ET-1, ICAM-1 and VCAM-1, and high sensitivity CRP. Results: The comparison between Cs…