Search results for "Ketoprofen"
showing 10 items of 38 documents
Glycation alters ligand binding, enzymatic, and pharmacological properties of human albumin.
2015
Albumin, the major circulating protein in blood plasma, can be subjected to an increased level of glycation in a diabetic context. Albumin exerts crucial pharmacological activities through its drug binding capacity, i.e., ketoprofen, and via its esterase-like activity, allowing the conversion of prodrugs into active drugs. In this study, the impact of the glucose-mediated glycation on the pharmacological and biochemical properties of human albumin was investigated. Aggregation product levels and the redox state were quantified to assess the impact of glycation-mediated changes on the structural properties of albumin. Glucose-mediated changes in ketoprofen binding properties and esterase-lik…
Direct chromatographic study of the enantioselective biodegradation of ibuprofen and ketoprofen by an activated sludge
2018
[EN] The quantification of the enantiomeric fraction (EF) during the biodegradation process is essential for environmental risk assessment. In this paper the enantioselective biodegradation of ibuprofen, IBU, and ketoprofen, KET, two of the drugs most consumed, was evaluated. Biodegradation experiments were performed in batch mode using a minimal salts medium inoculated with an activated sludge (collected from a Valencian Waste Water Treatment Plant) and supplemented with the racemate of each compound. The inoculum activity was verified using fluoxetine as reference compound. The experimental conditions used (analyte concentration and volume of inoculum) were chosen according to OECD guidel…
Determination of non-steroidal anti-inflammatory drugs in water and urine using selective molecular imprinted polymer extraction and liquid chromatog…
2016
Abstract A selective solid-phase extraction was employed for the improvement of the determination of non-steroidal anti-inflammatory drugs (NSAIDs) in continental water and urine samples. Ketoprofen, naproxen, diclofenac, and ibuprofen were selected as target analytes due to they are the most frequently administered and consumed NSAIDs. These compounds were extracted using molecular imprinted polymers and determined by liquid chromatography with diode array (DAD), and tandem-mass spectrometry (MS–MS) detectors. Performance of DAD and MS–MS detectors was evaluated throughout this study. The obtained limits of quantification, after a 50-fold preconcentration solid-phase extraction, varied fro…
Preclinical Effect of Absorption Modifying Excipients on Rat Intestinal Transport of Model Compounds and the Mucosal Barrier Marker 51Cr-EDTA
2017
There is a renewed interest from the pharmaceutical field to develop oral formulations of compounds, such as peptides, oligonucleotides, and polar drugs. However, these often suffer from insufficient absorption across the intestinal mucosal barrier. One approach to circumvent this problem is the use of absorption modifying excipient(s) (AME). This study determined the absorption enhancing effect of four AMEs (sodium dodecyl sulfate, caprate, chitosan, N-acetylcysteine) on five model compounds in a rat jejunal perfusion model. The aim was to correlate the model compound absorption to the blood-to-lumen clearance of the mucosal marker for barrier integrity, 51Cr-EDTA. Sodium dodecyl sulfate a…
Relation between structural and release properties in a polysaccharide gel system.
2007
Abstract The potential utility of κ-carrageenan gels for preparing drug release devices is here shown. Structural properties of κ-carrageenan gels prepared with different salt composition and containing Ketoprofen sodium salt, as model drug, have been evaluated with static light scattering and rheological measurements. These properties have been correlated with release profiles in vitro at pH 5.5. Release properties from gelled matrices have been compared with those obtained by two commercial products containing the same drug. Results show that: i) in this system it is possible to easily control the gel texture by using different cationic concentration; ii) the kinetics of drug release by κ…
Influence of cryogenic grinding on properties of a self-emulsifying formulation
2003
Abstract Recently, self-emulsifying drug delivery systems (SEDDS) have been developed as a method to deliver lipophilic drugs. Gelucire® 44/14 is an excipient, from the lauroyl macrogolglycerides family, producing a fine oil-in-water emulsion when introduced into an aqueous phase under gentle agitation as SEDDS, improving thereby solubility of poorly water-soluble drugs and their bioavailability. The aims of this study were to process Gelucire® 44/14 into a powder by cryogenic grinding to produce solid oral dosage forms and to investigate influence of this process on different properties of a formulation made of Gelucire® 44/14 and ketoprofen (90/10). Cryogenic grinding produced Gelucire® 4…
Comparison of the efficacy of low doses of methylprednisolone, acetaminophen, and dexketoprofen trometamol on the swelling developed after the remova…
2015
WOS: 000365269900016
Quality control of pharmaceuticals containing non-steroidal anti-inflammatory drugs by micellar liquid chromatography
2002
A liquid chromatographic procedure is described for the determination of acemetacin, diclofenac, indomethacin, ketoprofen, nabumetone, naproxen, piketoprofen, and tolmentin in pharmaceutical preparations. The compounds were separated on a Kromasil C18 analytical column, with a guard column of similar characteristics; the mobile phase was 0.06 M cetyltrimethylammonium bromide, at pH 7, containing 10% 1-butanol. At a flow rate of 1 mL min−1 the elution time of the most retained compound was 23 min. Limits of detection were between 0.01 μ g mL−1 for diclofenac and 0.2 μ g mL−1 for naproxen. The proposed method enables the determination of non-steroidal anti-inflammatory drugs in pharmaceutical…
Distinguishing the roles of carrier and biofilm in filtering media for the removal of pharmaceutical compounds from wastewater
2017
Abstract Pharmaceutical compounds (PCs) represent a large group of micropollutants in wastewaters (WW) worldwide. Many PCs are resistant to conventional WW treatment. Moreover, for some PCs the removal process is reversible. The aim of this study was to test three newly developed ceramic carriers for biofilm formation and PCs removal. The testing of untreated WW samples by liquid chromatography coupled to Orbitrap mass spectrometry allowed to detect the occurrence of 18 PCs in the range from 26 ng/L to 20,688 ng/L, with the highest concentrations found for caffeine. Besides, among PCs with concentrations above 1,000 ng/L, ibuprofen (19,234 ng/L), naproxen (1,405 ng/L), xylazine (1,366 ng/L)…
Comparison between sodium dodecylsulphate and cetyltrimethylammonium bromide as mobile phases in the micellar liquid chromatography determination of …
2004
The retention behaviour of non-steroidal anti-inflammatory drugs (NSAIDs) using micellar mobile phases of sodium dodecylsulphate (SDS) is studied and compared with that observed with micellar mobile phases of cetyltrimethylammonium bromide (CTAB). A liquid chromatographic procedure for the determination of acemetacin, diclofenac, indomethacin, ketoprofen, naproxen and tolmetin in pharmaceutical preparations is described. The proposed system uses a Kromasil C18 analytical column and a solution of 0.15 M SDS at pH 3 with 10% 1-propanol as mobile phase. Under these conditions, the studied NSAIDs elute between 6 and 10 min at a 1 mL min(-1) flow rate. Limits of detection (LOD) are lower than 0.…