Search results for "Kiev"

Early entry events in Echovirus 30 infection

2020

Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, surprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 strain Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for successful infection, but not the coxsackievirus and adenovirus receptor (CAR) or decay-accelerating factor (DAF), although an interaction with …

2019

Abstract Type B Coxsackieviruses (CVBs) belong to the enterovirus genus, and they cause both acute and chronic diseases in humans. CVB infections usually lead to flu-like symptoms but can also result in more serious diseases such as myocarditis, aseptic meningitis and life-threatening multi-organ infections in young infants. Thus, CVBs have long been considered as important targets of future vaccines. We have previously observed CVB1 capsid disintegration and virus concentration decrease with 12-day long formalin inactivation protocol. Here a scalable ion exchange chromatography purification method was developed, and purified CVB1 was inactivated with UV-C or formalin. Virus morphology and …

Kawalerzysta, dowódca i wykładowca - szkic do biografii gen. bryg. Konstantego Druckiego-Lubeckiego (1893-1940)

2018

Celem przeprowadzonych badań było opracowanie rozszerzonego szkicu biograficznego księcia gen. bryg. Konstantego Druckiego-Lubeckiego. Dzięki zebranym dokumentom i materiałom ustalono najważniejsze fakty z życia generała, dotyczące jego pochodzenia, nauki w Cesarskim Aleksandrowskim Liceum (Lycee Imperial Alexandre) w Petersburgu, udziału w walkach o niepodległość Polski oraz kariery w kawalerii i szkolnictwie wojskowym II RP. Udostępnione przez bliskich księcia materiały, skonfrontowane ze źródłami urzędowymi i dostępnymi publikacjami, pozwoliły także na ustalenie dodatkowych okoliczności związanych z jego śmiercią z rąk NKWD w 1940 roku. Dokumenty te umożliwiły rekonstrukcję podejmowanych…

Virus-receptor interactions of coxsackie B viruses and their putative influence on cardiotropism

2003

Specific virus-receptor interactions are important determinants in the pathogenesis of viral infections, influencing the location and initiation of primary infection as well as the viral spread to other target organs in the postviremic phase. Coxsackieviruses of group B (CVB) specifically interact with at least two receptor proteins, the coxsackievirus-adenovirus receptor (CAR) and the decay-accelerating factor (DAF), and cause a broad spectrum of diseases, including acute and chronic myocarditis. In the human heart, CAR is predominantly expressed in intercalated discs, regions of utmost importance for the functional integrity of the heart. Since DAF is abundantly expressed in epithelial an…

A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses

2018

AbstractEnteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybr…

Rodzina Andrzejowskich i ich rola w zachowaniu polskiej tożsamości narodowej na Ukrainie na przykładzie aktywności oświatowo-wychowawczej Józefata An…

2019

The purpose of the article is to recall the history of Polish families in Eastern Europe, and strictly in Ukraine at the end of the 19th and the beginning of the 20th century. In a short article, however, it is difficult to tell their history in full. However, you can look at them from the perspective of individual stories, as in the case of Józefat Andrzejowski, who is active in economic and educational and educational grounds. He was born in 1849 in Kielce as the son of a November insurgent. After graduating from junior high school in Kielce, he went to Switzerland to study ceramics, where he was strongly influenced by Andrzej Towiański. In 1878 he moved to Kiev and founded a ceramics fac…

A hexavalent Coxsackievirus B vaccine is highly immunogenic and has a strong protective capacity in mice and nonhuman primates

2020

A proof-of-concept study shows the generation of an immunogenic hexavalent Coxsackie B virus vaccine that prevents disease.

2020

Coxsackievirus B (CVB) enteroviruses are common human pathogens known to cause severe diseases including myocarditis, chronic dilated cardiomyopathy, and aseptic meningitis. CVBs are also hypothesized to be a causal factor in type 1 diabetes. Vaccines against CVBs are not currently available, and here we describe the generation and preclinical testing of a novel hexavalent vaccine targeting the six known CVB serotypes. We show that the vaccine has an excellent safety profile in murine models and nonhuman primates and that it induces strong neutralizing antibody responses to the six serotypes in both species without an adjuvant. We also demonstrate that the vaccine provides immunity against …

Enterovirus Co-infections and Onychomadesis after Hand, Foot, and Mouth Disease, Spain, 2008

2011

Mixed infection of enteroviruses may explain the rare complication of nail shedding. Onychomadesis after HFMD

Internalization of coxsackievirus A9 is mediated by {beta}2-microglobulin, dynamin, and Arf6 but not by caveolin-1 or clathrin.

2010

ABSTRACT Coxsackievirus A9 (CAV9) is a member of the human enterovirus B species within the Enterovirus genus of the family Picornaviridae . It has been shown to utilize αV integrins, particularly αVβ6, as its receptors. The endocytic pathway by which CAV9 enters human cells after the initial attachment to the cell surface has so far been unknown. Here, we present a systematic study concerning the internalization mechanism of CAV9 to A549 human lung carcinoma cells. The small interfering RNA (siRNA) silencing of integrin β6 subunit inhibited virus proliferation, confirming that αVβ6 mediates the CAV9 infection. However, siRNAs against integrin-linked signaling molecules, such as Src, Fyn, R…