Search results for "Kir2.1"

showing 2 items of 2 documents

Characterisation of K<sup>+</sup> Currents in the C8-B4 Microglial Cell Line and their Regulation by Microglia Activating Stimuli

2009

Microglia are the intrinsic immune cells of the brain. As such, they are crucially involved in neuro-protection as well as neuro-degeneration. Their activation leads to the induction of cytokine and chemokine release, the production of reactive oxygen species and nitric oxide and an increased outward potassium conductance. In this study, we focus our interest on potassium currents and channels in the C8-B4 murine microglial cell line and compare them with those of primary cultured microglia from neo-natal mice. Using the whole cell patch-clamp technique, we have recorded prominent inward and outward rectifying voltage-dependent potassium currents but no calcium-activated potassium currents.…

Membrane potentialMicrogliaPhysiologyChemistryPotassiumMargatoxinKir2.1chemistry.chemical_elementPotassium channel blockerPotassium channelCell biologymedicine.anatomical_structureBiochemistrymedicinePatch clampmedicine.drugCellular Physiology and Biochemistry
researchProduct

Microglia and α-synuclein implication in Parkinson's disease

2011

Age-related neurodegenerative disorders like Parkinson’s disease take an enormous toll on individuals and on society. Despite extensive efforts, Parkinson’s disease remains incurable and only very limited treatments exist. Indeed, Parkinson’s pathogenesis is still not clear and research on its molecular mechanisms is ongoing. In this study, we focused our interest on two abnormal events occurring in Parkinson’s patients, namely α-synuclein aggregation and microglial activation. We first investigated α-synuclein and its abnormal polymerisation. For this purpose, we developed novel methods, which allowed the in vitro production of different types of α-synuclein oligomers. Using highly sensiti…

[SDV.SA]Life Sciences [q-bio]/Agricultural sciencesΑ-synucléineAgingMaladie de ParkinsonDopamineCultures primairesBrain immunityNeuronesVieillissementImmunité du cerveauPotassium channelsNeuroinflammationToxicitéC8-B4 cell line[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathologyMéthode d’isolation in vitroElectrophysiologieCanaux potassiquesNeurodegeneration[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciencesIn vitro isolation methodPrimary cultureKv1.3 - Kir2.1Neurons[SDV.SA] Life Sciences [q-bio]/Agricultural sciences[SDV.MHEP] Life Sciences [q-bio]/Human health and pathologyToxicityMaladies neurodégénérativesΑ-synucleinNitric oxideNeurodégénérationOligomèresElectrophysiologyMicrogliesBrain macrophagesOligomersOxyde nitriqueNeurodegenerative disordersParkinson’s diseaseCytokinesLignée cellulaire C8-B4MicrogliaPatch-clamp[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
researchProduct