Search results for "LAB"
showing 10 items of 7932 documents
Pharmacological targeting of the novel β-catenin chromatin-associated kinase p38α in colorectal cancer stem cell tumorspheres and organoids
2021
AbstractThe prognosis of locally advanced colorectal cancer (CRC) is currently unsatisfactory. This is mainly due to drug resistance, recurrence, and subsequent metastatic dissemination, which are sustained by the cancer stem cell (CSC) population. The main driver of the CSC gene expression program is Wnt signaling, and previous reports indicate that Wnt3a can activate p38 MAPK. Besides, p38 was shown to feed into the canonical Wnt/β-catenin pathway. Here we show that patient-derived locally advanced CRC stem cells (CRC-SCs) are characterized by increased expression of p38α and are “addicted” to its kinase activity. Of note, we found that stage III CRC patients with high p38α levels display…
Desferrioxamine as an appropriate chelator for 90Nb: Comparison of its complexation properties for M-Df-Octreotide (M=Nb, Fe, Ga, Zr)
2014
The niobium-90 radioisotope ((90)Nb) holds considerable promise for use in immuno-PET, due to its decay parameters (t½ = 14.6h, positron yield=53%, Eß(+)(mean) = 0.35 MeV and Eß(+)(max) = 1.5 MeV). In particular, (90)Nb appears well suited to detect in vivo the pharmacokinetics of large targeting vectors (50-150 kDa). In order to be useful for immuno-PET chelators are required to both stabilize the radionuclide in terms of coordination chemistry and to facilitate the covalent attachment to the targeting vector. Different chelators were evaluated for this purpose in terms of radiolabelling efficiency and stability of the radiolabelled Nb(V) complex and in order to determine the most suitable…
Open-label extension study of the RNAi therapeutic ALN-VSP02 in cancer patients responding to therapy.
2012
3062 Background: ALN-VSP02 is an RNA interference (RNAi) therapeutic comprised of lipid nanoparticle-formulated small interfering RNAs targeting vascular endothelial growth factor (VEGF)-A and kinesin spindle protein (KSP). In a phase 1 trial, ALN-VSP02 administered as an iv infusion q2 wks was well-tolerated and showed evidence of anti-VEGF pharmacology and antitumor activity. Methods: Patients treated on the phase I trial with stable disease (SD) or better after 4 months (8 doses) were eligible to continue on an extension study until disease progression. Main objectives included continued evaluation of safety/tolerability and assessment of disease response. Results: Seven of 37 patients …
Synthesis and PET studies of [11C-cyano]letrozole (Femara®), an aromatase inhibitor drug
2009
Abstract Introduction Aromatase, a member of the cytochrome P 450 family, converts androgens such as androstenedione and testosterone into estrone and estradiol, respectively. Letrozole (1-[bis-(4-cyanophenyl)methyl]-1 H -1,2,4-triazole; Femara) is a high-affinity aromatase inhibitor ( K i =11.5 nM) that has Food and Drug Administration approval for breast cancer treatment. Here we report the synthesis of carbon-11-labeled letrozole and its assessment as a radiotracer for brain aromatase in the baboon. Methods Letrozole and its precursor (4-[(4-bromophenyl)-1 H -1,2,4-triazol-1-ylmethyl]benzonitrile) were prepared in a two-step synthesis from 4-cyanobenzyl bromide and 4-bromobenzyl bromide,…
Posaconazole against Candida glabrata with Variable Susceptibility to Fluconazole
2008
We investigated the in vitro activities of posaconazole (POS), fluconazole (FLC), amphotericin B (AMB), and caspofungin (CAS) against four clinical isolates of Candida glabrata with various susceptibilities to FLC (FLC MICs ranging from 1.0 to >64 microg/ml). POS MICs ranged from 64 microg/ml; POS MIC of 0.5 microg/ml). FLC significantly reduced the kidney burden of mice infected with the S strain (P = 0.0070) but not of those infected with the S-DD and R strains. POS was significantly effective against all three isolates at reducing the kidney fungal burden with respect to the controls (P ranging from 0.0003 to 0.029). In conclusion, our data suggest that POS may be a useful option in the …
Dog sperm head morphometry: its diversity and evolution
2017
Dogs have been under strong artificial selection as a consequence of their relationship with man. Differences between breeds are evident that could be reflected in seminal characteristics. The present study was to evaluate differences in sperm head morphometry between seven well-defined breeds of dog: the British Bulldog, Chihuahua, German Shepherd, Labrador Retriever, Spanish Mastiff, Staffordshire Terrier, and Valencian Rat Hunting dog. Semen samples were obtained by masturbation and smears stained with Diff-Quik. Morphometric analysis (CASA-Morph) produced four size and four shape parameters. Length, Ellipticity, and Elongation showed higher differences between breeds. MANOVA revealed di…
Evaluation of Small Vase and Y-trellis Orchard Systems for Peach and Nectarine Production in Mediterranean Regions
2015
Two peach planting systems, Small Vase (SV) and Y-trellis (Y), were evaluated and compared in the Mediterranean settings of Southern Italy. The two orchards were located next to each other on relatively uniform soil and terrain, and the observations included two peach (âRich Mayâ and âSummer Richâ) and two nectarine (âBig Bangâ and âNectarossâ) cultivars. In the SV system, trees were spaced at 4.5 A 2.5 m (888 trees/ha), whereas in the Y system, trees were spaced at 5.5 A 2 m (909 trees/ha) and no canopy gap was left between rows. Yield per tree; fruit size grade; unit price of sold peaches for each size grade; materials, labor and associated costs for cultural management;…
Influence of Component Temperature Derivation from Dual Angle Thermal Infrared Observations on TSEB Flux Estimates Over an Irrigated Vineyard
2015
A two-source model for deriving surface energy fluxes and their soil and canopy components was evaluated using multi-angle airborne observations. In the original formulation (TSEB1), a single temperature observation, Priestley–Taylor parameterization and the vegetation fraction are used to derive the component fluxes. When temperature observations are made from different angles, soil and canopy temperatures can be extracted directly. Two dual angle model versions are compared versus TSEB1: one incorporating the Priestley–Taylor parameterization (TSEB2I) and one using the component temperatures directly (TSEB2D), for which data from airborne campaigns over an agricultural area in Spain are u…
PALERMO. CANTIERI CULTURALI ALLA ZISA 2.0. ZISALAB_AZIONI, PROGETTI E VISIONI PER UN PROGETTO URBANO
2013
Gli esiti di ZISA_Lab, un workshop di progettazione architettonica e urbana che ha visto coinvolti università, amministratori, artisti e makers sociali e culturali come attori principali di sperimentazioni progettuali, alcune delle quali realizzate al termine del workshop stesso. Uno spazio pubblico in trasformazione con vocazione culturale ha ospitato un gruppo di docenti e studenti, dando vita ad alcune operazioni di trasformazioni di uno spazio aperto e all'allestimento di una mostra realizzato in condivisione e in co-progettazione con gli artisti, realizzato all'interno di ZAC il nuovo padiglione per l'arte contemporanea della città
Preliminary data concerning the morphology of a Calabrian Ionian margin area: Caulonia and Marina di Gioiosa canyons
2008
In the framework of the Vector National Italian Project (VulCost line), aimed to study the role of the morphology and the geology of the Ionian Calabrian margin in the coastline evolution, an oceanographic cruise was planned to collect geophysical data along two canyon systems: Caulonia and Marina di Gioiosa. The survey explored the continental shelf and slope from a depth of 15 m to more than 1150 m, using Multibeam Echosounder to investigate the seafloor topography. This work provides an outline of the erosive feature of the slope, shaped mostly by seasonal river input and by the connection to the structural and geological characteristics of the margin, made interesting by a narrow shelf …