Search results for "LAB"

showing 10 items of 7932 documents

Sentinel hospital-based surveillance for norovirus infection in children with gastroenteritis between 2015 and 2016 in Italy

2018

Noroviruses are one of the leading causes of gastro-enteric diseases worldwide in all age groups. Novel epidemic noroviruses with GII.P16 polymerase and GII.2 or GII.4 capsid type have emerged worldwide in late 2015 and in 2016. We performed a molecular epidemiological study of the noroviruses circulating in Italy to investigate the emergence of new norovirus strains. Sentinel hospital-based surveillance, in three different Italian regions, revealed increased prevalence of norovirus infection in children (<15 years) in 2016 (14.4% versus 9.8% in 2015) and the emergence of GII.P16 strains in late 2016, which accounted for 23.0% of norovirus infections. The majority of the strains with a GII.…

0301 basic medicineRNA virusesEuropean PeopleSettore MED/07 - Microbiologia E Microbiologia Clinicavirusesmedicine.disease_causePathology and Laboratory MedicinePediatricsGeographical locationsfluids and secretionsEpidemiologyGenotypePrevalenceMedicine and Health SciencesEthnicitiesChildCaliciviridae InfectionsMultidisciplinaryIncidence (epidemiology)Database and informatics methodsQRSequence analysisvirus diseasesGastroenteritisItalian PeopleEuropeCapsidItalyMedical MicrobiologyChild PreschoolViral PathogensVirusesMedicineRNA ViralPathogensPediatric InfectionsResearch Articlemedicine.medical_specialtyGenotypingGenotypeBioinformaticsScience030106 microbiologySequence DatabasesMicrobiologyCaliciviruses03 medical and health sciencesAge groupsmedicineHumansEuropean UnionMolecular Biology TechniquesGenotypingMicrobial PathogensMolecular BiologyBiochemistry Genetics and Molecular Biology (all)RNA sequence analysisBiology and life sciencesbusiness.industrySequence Analysis RNANorovirusOrganismsGenetic VariationRNA-Dependent RNA PolymeraseVirologydigestive system diseasesResearch and analysis methods030104 developmental biologyCaliciviridae InfectionsBiological DatabasesAgricultural and Biological Sciences (all)NorovirusCapsid ProteinsPopulation GroupingsPeople and placesbusinessSentinel Surveillance
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Identification of a large, fast-expanding HIV-1 subtype B transmission cluster among MSM in Valencia, Spain

2017

We describe and characterize an exceptionally large HIV-1 subtype B transmission cluster occurring in the Comunidad Valenciana (CV, Spain). A total of 1806 HIV-1 protease-reverse transcriptase (PR/RT) sequences from different patients were obtained in the CV between 2004 and 2014. After subtyping and generating a phylogenetic tree with additional HIV-1 subtype B sequences, a very large transmission cluster which included almost exclusively sequences from the CV was detected (n = 143 patients). This cluster was then validated and characterized with further maximum-likelihood phylogenetic analyses and Bayesian coalescent reconstructions. With these analyses, the CV cluster was delimited to 11…

0301 basic medicineRNA virusesMaleEpidemiologyLineage (evolution)lcsh:MedicineHIV InfectionsPathology and Laboratory MedicineMen who have sex with menCoalescent theorylaw.inventionGeographical LocationsDatabase and Informatics MethodsImmunodeficiency VirusesHIV ProteaselawMedicine and Health Scienceslcsh:ScienceValenciaPhylogenyMultidisciplinarybiologyPhylogenetic treePhylogenetic AnalysisHIV diagnosis and managementSubtypingHIV Reverse TranscriptaseVirusEuropeTransmission (mechanics)Medical MicrobiologyHIV epidemiologyViral PathogensVirusesFemalePathogensMalalties de transmissió sexualSequence AnalysisResearch ArticleBioinformaticsDisease clusterResearch and Analysis MethodsMicrobiology03 medical and health sciencesRetrovirusesDrug Resistance ViralHumansHomosexuality MaleMolecular Biology TechniquesMicrobial PathogensMolecular BiologyDemographyMolecular Biology Assays and Analysis Techniqueslcsh:RLentivirusOrganismsBiology and Life SciencesHIVbiology.organism_classificationDiagnostic medicine030104 developmental biologySpainpol Gene Products Human Immunodeficiency VirusPeople and PlacesHIV-1lcsh:QSequence AlignmentDemography
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Cosavirus, Salivirus and Bufavirus in Diarrheal Tunisian Infants

2016

International audience; Three newly discovered viruses have been recently described in diarrheal patients: Cosa-virus (CosV) and Salivirus (SalV), two picornaviruses, and Bufavirus (BuV), a parvovirus. The detection rate and the role of these viruses remain to be established in acute gastroen-teritis (AGE) in diarrheal Tunisian infants. From October 2010 through March 2012, stool samples were collected from 203 children <5 years-old suffering from AGE and attending the Children's Hospital in Monastir, Tunisia. All samples were screened for CosV, SalV and BuV as well as for norovirus (NoV) and group A rotavirus (RVA) by molecular biology. Positive samples for the three screened viruses were …

0301 basic medicineRNA virusesMaleRotavirusViral DiseasesHuman astrovirusesIdentificationvirusesEnteric viruseslcsh:MedicinePolymerase chain-reactionArtificial Gene Amplification and ExtensionPicornaviridaemedicine.disease_causePathology and Laboratory MedicinePolymerase Chain ReactionPediatricsReverse-transcription-pcrlaw.inventionParvoviruslaw[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesRotavirusMedicine and Health Scienceslcsh:ScienceChildrenPolymerase chain reactionPhylogenyMultidisciplinarybiologyTransmission (medicine)[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringPhylogenetic Analysis3. Good healthGastroenteritisInfectious DiseasesMedical MicrobiologyViral PathogensChild PreschoolViruses[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyFemalePathogensAichi virusPediatric InfectionsResearch ArticleDiarrheaTunisia030106 microbiologyGastroenterology and HepatologyResearch and Analysis MethodsMicrobiologyCalicivirusesAstrovirus03 medical and health sciencesKlassevirusParvovirusesAdeno-Associated VirusesmedicineHumansMolecular Biology TechniquesMolecular BiologyMicrobial PathogensRotavirus InfectionMolecular Biology Assays and Analysis TechniquesParvovirusNoroviruslcsh:ROrganismsBiology and Life SciencesInfantSapovirusReverse Transcriptase-Polymerase Chain Reactionbiology.organism_classificationVirology030104 developmental biologyNoroviruslcsh:QDNA viruses
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Impact of PNPLA3 and IFNL3 polymorphisms on hepatic steatosis in Asian patients with chronic hepatitis C.

2017

Background and aims A recent meta-analysis revealed that the genotype PNPLA3 rs738409 GG is associated with a higher risk of hepatic steatosis (HS) in Caucasian patients with chronic hepatitis C (CHC). However, controversial results were found regarding Asian populations. Furthermore, previous studies have shown a negative association between interferon lambda 3 (IFNL3) rs12979860 CC and HS in Caucasian CHC patients, but there have been no reports indicating any such association in Asian populations. In this study, then, we investigated the association of PNPLA3 and IFNL3 polymorphisms with HS in Asian CHC patients. Methods We enrolled consecutive CHC patients who underwent liver biopsy pri…

0301 basic medicineRNA virusesMaleSteatosisHeredityPhysiologylcsh:MedicineHepacivirusChronic liver diseasePathology and Laboratory MedicineGastroenterologyBody Mass IndexCytopathologyLiver disease0302 clinical medicineEndocrinologyGenotypeMedicine and Health Scienceslcsh:ScienceMultidisciplinaryAlcohol Consumptionmedicine.diagnostic_testHepatitis C virusFatty liverHepatitis CMedical microbiologyMiddle AgedGenetic MappingPhysiological ParametersLiverLiver biopsyViruses030211 gastroenterology & hepatologyFemalePathogensResearch ArticleAdultmedicine.medical_specialtyEndocrine DisordersVariant GenotypesMicrobiologyPolymorphism Single Nucleotide03 medical and health sciencesAsian PeopleInternal medicinemedicineGeneticsDiabetes MellitusHumansGenetic Predisposition to DiseaseAllelesGenetic Association StudiesNutritionAgedFlavivirusesbusiness.industryInterleukinsBody Weightlcsh:ROrganismsViral pathogensBiology and Life SciencesMembrane ProteinsLipaseHepatitis C Chronicmedicine.diseaseFibrosisHepatitis virusesDietMicrobial pathogensFatty Liver030104 developmental biologyAnatomical PathologyGenetic LociMetabolic Disorderslcsh:QInterferonsSteatosisbusinessBody mass indexDevelopmental BiologyPLoS ONE
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NESSie.jl – Efficient and intuitive finite element and boundary element methods for nonlocal protein electrostatics in the Julia language

2018

Abstract The development of scientific software can be generally characterized by an initial phase of rapid prototyping and the subsequent transition to computationally efficient production code. Unfortunately, most programming languages are not well-suited for both tasks at the same time, commonly resulting in a considerable extension of the development time. The cross-platform and open-source Julia language aims at closing the gap between prototype and production code by providing a usability comparable to Python or MATLAB alongside high-performance capabilities known from C and C++ in a single programming language. In this paper, we present efficient protein electrostatics computations a…

0301 basic medicineRapid prototypingGeneral Computer Sciencebusiness.industryComputer scienceComputationUsabilityPython (programming language)Finite element methodTheoretical Computer ScienceNESSIEComputational science03 medical and health sciences030104 developmental biologyModeling and SimulationbusinessMATLABBoundary element methodcomputercomputer.programming_languageJournal of Computational Science
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HUMAN T-LYMPHOTROPIC VIRUS 1 (HTLV-1) AND HUMAN T-LYMPHOTROPIC VIRUS 2 (HTLV-2): GEOGRAPHICAL RESEARCH TRENDS AND COLLABORATION NETWORKS (1989-2012)

2016

Publications are often used as a measure of research work success. Human T-lymphotropic virus (HTLV) type 1 and 2 are human retroviruses, which were discovered in the early 1980s, and it is estimated that 15-20 million people are infected worldwide. This article describes a bibliometric review and a coauthorship network analysis of literature on HTLV indexed in PubMed in a 24-year period. A total of 7,564 documents were retrieved, showing a decrease in the number of documents from 1996 to 2007. HTLV manuscripts were published in 1,074 journals. Japan and USA were the countries with the highest contribution in this field (61%) followed by France (8%). Production ranking changed when the numb…

0301 basic medicineResearch groupsBiomedical Researchlcsh:Arctic medicine. Tropical medicinelcsh:RC955-962030231 tropical medicinePopulationBibliometricsGlobal HealthGross domestic product03 medical and health sciences0302 clinical medicineHuman T-lymphotropic virus (HTLV)Global healthMedicineHumansCooperative BehaviorSocioeconomicseducationeducation.field_of_studyHuman T-lymphotropic virus 1biologyGeographybusiness.industryHuman T-lymphotropic virus 2Tropical spastic paraparesisGeneral Medicinebiology.organism_classificationHTLV-I InfectionsT cell leukemia/lymphoma030104 developmental biologyInfectious DiseasesGross national incomeBibliometricsHuman T-lymphotropic virus 1Human T-lymphotropic virus 2ImmunologyOriginal ArticlePeriodicals as TopicbusinessResearch collaboration
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Peripherin-2 differentially interacts with cone opsins in outer segments of cone photoreceptors

2016

Peripherin-2 is a glycomembrane protein exclusively expressed in the light-sensing compartments of rod and cone photoreceptors designated as outer segments (OS). Mutations in peripherin-2 are associated with degenerative retinal diseases either affecting rod or cone photoreceptors. While peripherin-2 has been extensively studied in rods, there is only little information on its supramolecular organization and function in cones. Recently, we have demonstrated that peripherin-2 interacts with the light detector rhodopsin in OS of rods. It remains unclear, however, if peripherin-2 also binds to cone opsins. Here, using a combination of co-immunoprecipitation analyses, transmission electron micr…

0301 basic medicineRhodopsinOpsingenetic structuresmacromolecular substances030105 genetics & heredityBiologymedicine.disease_causeRetinaMice03 medical and health scienceschemistry.chemical_compoundImmunolabelingMicroscopy Electron TransmissionAntigens NeoplasmFluorescence Resonance Energy TransferGeneticsmedicineAnimalsHumansPeripherin 2Molecular BiologyGenetics (clinical)MutationRetinal DegenerationRetinalGeneral MedicineCone Opsinseye diseases030104 developmental biologyFörster resonance energy transfernervous systemchemistryRhodopsinMutationRetinal Cone Photoreceptor CellsBiophysicsbiology.proteinsense organsProtein BindingVisual phototransductionHuman Molecular Genetics
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Oral administration of Bifidobacterium bifidum G9-1 alleviates rotavirus gastroenteritis through regulation of intestinal homeostasis by inducing muc…

2017

Human rotavirus (RV) infection is a leading cause of dehydrating diarrhea in infants and young children worldwide. Since therapeutic approaches to RV gastroenteritis are limited to alleviation of dehydration with oral rehydration solutions, more direct approaches to palliate symptoms of RV gastroenteritis are required. Treatments with probiotics have been increasingly recognized as alternative safe and low cost treatments for moderate infectious diarrhea. In this study, Bifidobacterium bifidum G9-1 (BBG9-1), which has been used as an intestinal drug for several decades, was shown to have a remarkable protective effect against RV gastroenteritis in a suckling mice model. As well as prophylac…

0301 basic medicineRotavirusMalabsorptionved/biology.organism_classification_rank.speciesAdministration Orallcsh:MedicinePathology and Laboratory MedicineBiochemistryCecumOral administrationChlorocebus aethiopsMedicine and Health SciencesLarge intestinelcsh:ScienceCecumGastrointestinal tractMice Inbred BALB CMultidisciplinaryGastroenteritisIntestinesDiarrheamedicine.anatomical_structureJejunumSmall Intestinemedicine.symptomAnatomyResearch ArticleDiarrheaGastroenterology and HepatologyRotavirus InfectionsCell Line03 medical and health sciencesSigns and SymptomsDiagnostic MedicineIleummedicineAnimalsBifidobacterium bifidumved/biologybusiness.industryProbioticslcsh:RBiology and Life SciencesProteinsmedicine.diseaseMacaca mulattaSmall intestineGastrointestinal Tract030104 developmental biologyGene Expression RegulationImmunologyMucinlcsh:QBifidobacterium bifidumbusinessDigestive SystemPLoS ONE
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GSaaS: A Service to Cloudify and Schedule GPUs

2018

Cloud technology is an attractive infrastructure solution that provides customers with an almost unlimited on-demand computational capacity using a pay-per-use approach, and allows data centers to increase their energy and economic savings by adopting a virtualized resource sharing model. However, resources such as graphics processing units (GPUs), have not been fully adapted to this model. Although, general-purpose computing on graphics processing units (GPGPU) is becoming more and more popular, cloud providers lack of flexibility to manage accelerators, because of the extended use of peripheral component interconnect (PCI) passthrough techniques to attach GPUs to virtual machines (VMs). F…

0301 basic medicineScheduleGeneral Computer ScienceComputer scienceDistributed computingnetworkingCloud computing02 engineering and technologycomputer.software_genre03 medical and health sciencesGPU resource management020204 information systems0202 electrical engineering electronic engineering information engineeringCloud computingGeneral Materials ScienceResource managementplatform virtualizationbusiness.industrycloud computingGeneral EngineeringVirtualizationShared resource030104 developmental biologyVirtual machineScalabilityGPU cloudificationlcsh:Electrical engineering. Electronics. Nuclear engineeringGeneral-purpose computing on graphics processing unitsbusinesscomputerlcsh:TK1-9971IEEE Access
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Fragments of peer review: A quantitative analysis of the literature (1969-2015)

2018

This paper examines research on peer review between 1969 and 2015 by looking at records indexed from the Scopus database. Although it is often argued that peer review has been poorly investigated, we found that the number of publications in this field doubled from 2005. A half of this work was indexed as research articles, a third as editorial notes and literature reviews and the rest were book chapters or letters. We identified the most prolific and influential scholars, the most cited publications and the most important journals in the field. Co-authorship network analysis showed that research on peer review is fragmented, with the largest group of co-authors including only 2.1% of the wh…

0301 basic medicineScience and Technology WorkforceResearch Quality Assessmentlcsh:MedicineCareers in ResearchPeer review co-authorship collaboration communityCitation analysisCentralityData MiningSociologylcsh:ScienceMultidisciplinary05 social sciencesScientometricsco-authorshipResearch AssessmentKnowledge sharingProfessionsCitation AnalysiscommunityNetwork AnalysisResearch ArticleComputer and Information SciencesScience PolicyAbstracting and IndexingPeer ReviewAbstracting and Indexing as Topic ; Animals ; Data Mining ; Databases Bibliographic ; History 20th Century ; History 21st Century ; Humans ; Peer ReviewScopusLibrary science050905 science studiesResearch and Analysis MethodsHistory 21st Century03 medical and health sciencesAnimalsHumansScientific Publishinglcsh:RScientometricsHistory 20th CenturyDatabases Bibliographiccollaboration030104 developmental biologyQuantitative analysis (finance)People and PlacesScientistslcsh:QPopulation Groupings0509 other social sciencesScientific publishingCentrality
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