Search results for "LABOR"

showing 10 items of 3876 documents

Caractérisation des enzymes du métabolisme des xénobiotiques olfactives chez le rat : régulation et rôle dans la détection périphérique des molécules…

2010

 Diplôme : Dr. d'Université; Les enzymes du métabolisme des xénobiotiques (EMX) jouent un rôle central dans la biotransformation et l’élimination de composés étrangers à l’organisme. Organisées en réseau de biotransformation, ces enzymes catalysent des réactions variées (oxydations, conjugaisons …) afin de rendre ces composés plus hydrophiles. Les EMX sont fortement exprimées dans l’épithélium olfactif de mammifères et des études in vitro ont montré qu’elles possèdent des activités élevées. Outre un rôle dans le métabolisme de molécules toxiques, ces enzymes pourraient être impliquées dans la modulation périphérique de l'olfaction. Les EMX pourraient contribuer à maintenir la sensibilité du…

RATS DE LABORATOIRECYTOCHROMES P450EVENEMENTS PERIRECEPTEURMOLECULES ODORANTESBIOTRANSFORMATION (METABOLISME)[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition[CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistryXENOBIOTIQUESOLFACTIONREGULATIONMETABOLISME DES XENOBIOTIQUESBIOTRANSFORMATIONODORAT[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
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Illumination correction on biomedical images

2014

RF-Inhomogeneity Correction (aka bias) artifact is an important re- search field in Magnetic Resonance Imaging (MRI). Bias corrupts MR images alter- ing their illumination even though they are acquired with the most recent scanners. Homomorphic Unsharp Masking (HUM) is a filtering technique aimed at correcting illumination inhomogeneity, but it produces a halo around the edges as a side effect. In this paper a novel correction scheme based on HUM is proposed to correct the artifact mentioned above without introducing the halo. A wide experimentation has been performed on MR images. The method has been tuned and evaluated using the simulated Brainweb image database. In this framework, the ap…

RF-inhomogeneity MRI magnetic resonance homomorphic unsharp masking bias artifactSettore ING-INF/05 - Sistemi Di Elaborazione Delle Informazioni
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Mapping inequalities in exclusive breastfeeding in low- and middle-income countries, 2000-2018

2021

Exclusive breastfeeding (EBF)—giving infants only breast-milk for the first 6 months of life—is a component of optimal breastfeeding practices effective in preventing child morbidity and mortality. EBF practices are known to vary by population and comparable subnational estimates of prevalence and progress across low- and middle-income countries (LMICs) are required for planning policy and interventions. Here we present a geospatial analysis of EBF prevalence estimates from 2000 to 2018 across 94 LMICs mapped to policy-relevant administrative units (for example, districts), quantify subnational inequalities and their changes over time, and estimate probabilities of meeting the World Health …

RJ101Psychological interventionBreastfeedingSocial SciencesgeographyBehavioral Neuroscience0302 clinical medicineRA0421PrevalencePsychologypolicy making030212 general & internal medicinePolicy Makinghumansmedia_commonCHILD GROWTH FAILUREDeveloping world0303 health scienceseducation.field_of_studyPsychology BiologicalGeographyPsychology ExperimentalHealth Status DisparitieMultidisciplinary SciencesGeographyBreast Feedingbreast feedingScale (social sciences)Science & Technology - Other TopicsLife Sciences & BiomedicineHumanAFRICASocial PsychologyInequalityspatial analysismedia_common.quotation_subjectpublic policyPopulationprevalencePublic policyDeveloping countryExperimental and Cognitive PsychologyPublic PolicyLocal Burden of Disease Exclusive Breastfeeding CollaboratorsArticleDeveloping Countrie03 medical and health sciencesEnvironmental healthHumansNutrition disorderseducationDeveloping Countries030304 developmental biologySpatial AnalysisScience & TechnologyNeurosciencesHealth Status Disparitiesdeveloping countrieshealth status disparitiesRisk factorsNeurosciences & NeurologyBreast feedingNature Human Behaviour
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The ER-Membrane Transport System Is Critical for Intercellular Trafficking of the NSm Movement Protein and Tomato Spotted Wilt Tospovirus.

2015

Plant viruses move through plasmodesmata to infect new cells. The plant endoplasmic reticulum (ER) is interconnected among cells via the ER desmotubule in the plasmodesma across the cell wall, forming a continuous ER network throughout the entire plant. This ER continuity is unique to plants and has been postulated to serve as a platform for the intercellular trafficking of macromolecules. In the present study, the contribution of the plant ER membrane transport system to the intercellular trafficking of the NSm movement protein and Tomato spotted wilt tospovirus (TSWV) is investigated. We showed that TSWV NSm is physically associated with the ER membrane in Nicotiana benthamiana plants. An…

RNA viruses0301 basic medicineLeavesCell MembranesNicotiana benthamianaPlant ScienceEndoplasmic ReticulumPathology and Laboratory MedicineBiochemistrySolanum lycopersicumTospovirusBunyavirusesMedicine and Health SciencesArabidopsis thalianaMovement proteinBiology (General)Integral membrane proteinSecretory PathwaybiologyPlant BiochemistryPlant AnatomyPlasmodesmataProteïnes de membranafood and beveragesPlantsPlants Genetically ModifiedCell biologyTransport proteinPlant Viral Movement ProteinsProtein TransportMedical MicrobiologyCell ProcessesViral PathogensVirusesPathogensCellular Structures and OrganellesTomato Spotted Wilt VirusResearch ArticleBioquímicaCell PhysiologyQH301-705.5Arabidopsis ThalianaImmunologyPlant PathogensBrassicaPlasmodesmaResearch and Analysis MethodsMicrobiologyPlant Viral Pathogens03 medical and health sciencesModel OrganismsPlant and Algal ModelsVirologyTobaccoGeneticsIntegral Membrane ProteinsSecretionMicrobial PathogensMolecular BiologyPlant DiseasesBiology and life sciencesEndoplasmic reticulumfungiOrganismsMembrane ProteinsCell BiologyPlant PathologyRC581-607biology.organism_classificationVirosis (Plantes)VirologyPlant Leaves030104 developmental biologyMembrane TraffickingParasitologyImmunologic diseases. AllergyPLoS Pathogens
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Exceptional Heterogeneity in Viral Evolutionary Dynamics Characterises Chronic Hepatitis C Virus Infection.

2016

The treatment of HCV infection has seen significant progress, particularly since the approval of new direct-acting antiviral drugs. However these clinical achievements have been made despite an incomplete understanding of HCV replication and within-host evolution, especially compared with HIV-1. Here, we undertake a comprehensive analysis of HCV within-host evolution during chronic infection by investigating over 4000 viral sequences sampled longitudinally from 15 HCV-infected patients. We compare our HCV results to those from a well-studied HIV-1 cohort, revealing key differences in the evolutionary behaviour of these two chronic-infecting pathogens. Notably, we find an exceptional level o…

RNA viruses0301 basic medicineMaleHepacivirusHIV InfectionsHepacivirusPathology and Laboratory Medicinemedicine.disease_causeVirus ReplicationHepatitis0302 clinical medicineImmunodeficiency VirusesMedicine and Health Sciences2.2 Factors relating to the physical environmentChronicAetiologylcsh:QH301-705.5Data Managementeducation.field_of_studybiologyHepatitis C virusLiver Diseasevirus diseasesHepatitis C3. Good healthPhylogeneticsInfectious DiseasesMedical MicrobiologyViral PathogensViral evolutionVirusesEvolutionary RateHIV/AIDS030211 gastroenterology & hepatologyFemalePathogensInfectionResearch Articlelcsh:Immunologic diseases. AllergyComputer and Information SciencesEvolutionary ProcessesEvolutionHepatitis C virusPopulationChronic Liver Disease and CirrhosisImmunologyMicrobiologyViral EvolutionVirusEvolution Molecular03 medical and health sciencesHepatitis - CVirologyRetrovirusesGeneticsmedicineHumansEvolutionary SystematicsEvolutionary dynamicseducationMicrobial PathogensMolecular BiologyTaxonomyEvolutionary BiologyFlavivirusesPopulation BiologyLentivirusOrganismsBiology and Life SciencesHIVMolecularHepatitis C Chronicbiology.organism_classificationVirologyHepatitis virusesOrganismal EvolutionViral ReplicationChronic infection030104 developmental biologyEmerging Infectious Diseaseslcsh:Biology (General)Viral replicationMicrobial EvolutionImmunologyHIV-1Parasitologylcsh:RC581-607Digestive DiseasesPopulation GeneticsFollow-Up Studies
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An unusually high substitution rate in transplant-associated BK polyomavirus in vivo is further concentrated in HLA-C-bound viral peptides

2018

Infection with human BK polyomavirus, a small double-stranded DNA virus, potentially results in severe complications in immunocompromised patients. Here, we describe the in vivo variability and evolution of the BK polyomavirus by deep sequencing. Our data reveal the highest genomic evolutionary rate described in double-stranded DNA viruses, i.e., 10−3–10−5 substitutions per nucleotide site per year. High mutation rates in viruses allow their escape from immune surveillance and adaptation to new hosts. By combining mutational landscapes across viral genomes with in silico prediction of viral peptides, we demonstrate the presence of significantly more coding substitutions within predicted cog…

RNA viruses0301 basic medicineMutation ratePhysiologyvirusesUrinePathology and Laboratory Medicinemedicine.disease_causeBiochemistryMedicine and Health SciencesBiology (General)Amino AcidsGenome EvolutionPhylogenyData ManagementMutationOrganic CompoundsHigh-Throughput Nucleotide SequencingPhylogenetic AnalysisDNA virusGenomicsBody FluidsBK virusPhylogeneticsChemistryMedical MicrobiologyViral PathogensViral evolutionVirusesPhysical SciencesEvolutionary RatePathogensAnatomyResearch ArticleComputer and Information SciencesEvolutionary ProcessesQH301-705.5ImmunologyGenome ViralHLA-C AntigensBiologyMicrobiologyMolecular EvolutionViral EvolutionVirusDeep sequencing03 medical and health sciencesVirologyGeneticsmedicineHumansEvolutionary SystematicsMicrobial PathogensMolecular BiologyTaxonomyEvolutionary BiologyPolyomavirus InfectionsOrganic ChemistryOrganismsChemical CompoundsBiology and Life SciencesComputational BiologyProteinsOrgan TransplantationRC581-607030112 virologyVirologyOrganismal EvolutionPeptide FragmentsPolyomaviruses030104 developmental biologyAmino Acid SubstitutionBK VirusMicrobial EvolutionMutationParasitologyImmunologic diseases. AllergyDNA virusesPolyomavirus Infections
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Human norovirus binding to select bacteria representative of the human gut microbiota

2016

Recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. The purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. The bacteria screened included naturally occurring strains isolated from human stool (Klebsiella spp., Citrobacter spp., Bacillus spp., Enterococcus faecium and Hafnia alvei) and select reference strains (Staphylococcus aureus and Enterobacter cloacae). Binding in PBS was evaluated to three human norovirus strains (GII.4 New Orleans 2…

RNA viruses0301 basic medicinePhysiologyvirusesEnterococcus faeciumFimbrialcsh:MedicineBacillusPathology and Laboratory Medicinemedicine.disease_causePilusFecesBinding AnalysisCitrobacterKlebsiellaMedicine and Health SciencesElectron Microscopylcsh:ScienceCitrobacterMicroscopyMultidisciplinarybiologyChemistryBody FluidsBloodMedical MicrobiologyViral PathogensVirusesAnaerobic bacteriaPathogensAnatomyCell Binding AssayResearch ArticleCell BindingStaphylococcus aureusCell PhysiologyAnaerobic BacteriaResearch and Analysis MethodsMicrobiologyCalicivirusesMicrobiology03 medical and health sciencesEnterobacter cloacaemedicineHumansMicrobial PathogensChemical CharacterizationBiology and life sciencesBacteriaNoroviruslcsh:ROrganismsHafnia alveiCell Biologybiology.organism_classificationCulture MediaGastrointestinal Microbiome030104 developmental biologyFimbriae BacterialNorovirusMicrobial InteractionsTransmission Electron Microscopylcsh:QEnterobacter cloacaeBacteriaEnterococcus faeciumPLOS ONE
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Cost-Effectiveness Analysis of Different Testing Strategies that Use Antibody Levels to Detect Chronic Hepatitis C in Blood Donors.

2016

Aim. We conducted a cost-effectiveness analysis of seven hepatitis C virus (HCV) testing strategies in blood donors. Methods. Three of the seven strategies were based on HCV diagnosis and reporting guidelines in Mexico and four were from previous and current recommendations outlined by the CDC. The strategies that were evaluated determine antibody levels according to the signal-to-cut-off (S/CO) ratio and use reflex Immunoblot (IMB) or HCV RNA tests to confirm true positive (TP) cases of chronic HCV infection. Costs were calculated from the perspective of the Mexican Institute of Social Security (IMSS). A decision tree model was developed to estimate the expected number of true positive cas…

RNA virusesDecision AnalysisPhysiologyEconomicsCost-Benefit AnalysisSocial Scienceslcsh:MedicineBlood DonorsHepacivirusmedicine.disease_causeBiochemistryHepatitis0302 clinical medicineImmune Physiology030212 general & internal medicineChroniclcsh:SciencePathology and laboratory medicinehealth care economics and organizationsMultidisciplinaryImmune System ProteinsCost–benefit analysisHepatitis C virusLiver DiseaseCost-effectiveness analysisMedical microbiologyHepatitis CHCV AntibodyInfectious DiseasesVirusesEngineering and TechnologyBlood Banks030211 gastroenterology & hepatologyPathogensInfectionManagement EngineeringResearch Articlemedicine.medical_specialtyGeneral Science & TechnologyHepatitis C virusImmunologyCost-Effectiveness AnalysisImmunoblottingChronic Liver Disease and CirrhosisMolecular Probe TechniquesAntibody levelResearch and Analysis MethodsMicrobiologyAntibodies03 medical and health sciencesChronic hepatitisHepatitis - CClinical ResearchInternal medicineparasitic diseasesmedicineHumansImmunoassaysMolecular Biology TechniquesSensitivity analysesMolecular BiologyMedicine and health sciencesBiology and life sciencesFlavivirusesbusiness.industryDecision Treeslcsh:RHepatitis C antibodyOrganismsViral pathogensProteinsHepatitis C ChronicHepatitis C AntibodiesHepatitis virusesEconomic AnalysisMicrobial pathogensHealth CareEmerging Infectious DiseasesCost Effectiveness ResearchHealth Care FacilitiesImmunologyImmunologic Techniqueslcsh:QbusinessDigestive Diseases
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APOBEC4 Enhances the Replication of HIV-1

2016

APOBEC4 (A4) is a member of the AID/APOBEC family of cytidine deaminases. In this study we found a high mRNA expression of A4 in human testis. In contrast, there were only low levels of A4 mRNA detectable in 293T, HeLa, Jurkat or A3.01 cells. Ectopic expression of A4 in HeLa cells resulted in mostly cytoplasmic localization of the protein. To test whether A4 has antiviral activity similar to that of proteins of the APOBEC3 (A3) subfamily, A4 was co-expressed in 293T cells with wild type HIV-1 and HIV-1 luciferase reporter viruses. We found that A4 did not inhibit the replication of HIV-1 but instead enhanced the production of HIV-1 in a dose-dependent manner and seemed to act on the viral L…

RNA virusesMale0301 basic medicineMolecular biologylcsh:MedicineArtificial Gene Amplification and ExtensionCytidinePathology and Laboratory MedicineVirus ReplicationBiochemistryPolymerase Chain ReactionJurkat cellschemistry.chemical_compoundCytidine deaminationImmunodeficiency VirusesTranscription (biology)TestisMedicine and Health Scienceslcsh:SciencePromoter Regions GeneticMultidisciplinaryCytidineTransfectionEnzymesImmunoblot AnalysisMedical MicrobiologyDeaminationViral PathogensViruses293T cellsCell linesPathogensOxidoreductasesBiological culturesLuciferaseResearch ArticleMolecular Probe TechniquesDNA constructionBiologyMicrobiologyCell Line03 medical and health sciencesCytidine DeaminaseRetrovirusesHumansMicrobial PathogensHIV Long Terminal Repeat030102 biochemistry & molecular biologylcsh:RLentivirusHEK 293 cellsOrganismsBiology and Life SciencesHIVProteinsPromoterMolecular biologyResearch and analysis methodsMolecular biology techniques030104 developmental biologychemistryPlasmid ConstructionHIV-1Enzymologylcsh:QEctopic expressionCloningPLOS ONE
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Impact of probiotic Saccharomyces boulardii on the gut microbiome composition in HIV-treated patients: A double-blind, randomised, placebo-controlled…

2017

Dysbalance in gut microbiota has been linked to increased microbial translocation, leading to chronic inflammation in HIV-patients, even under effective HAART. Moreover, microbial translocation is associated with insufficient reconstitution of CD4+T cells, and contributes to the pathogenesis of immunologic non-response. In a double-blind, randomised, placebo-controlled trial, we recently showed that, compared to placebo, 12 weeks treatment with probiotic Saccharomyces boulardii significantly reduced plasma levels of bacterial translocation (Lipopolysaccharide-binding protein or LBP) and systemic inflammation (IL-6) in 44 HIV virologically suppressed patients, half of whom (n = 22) had immun…

RNA virusesMale0301 basic medicinePlacebo-controlled studylcsh:MedicineHIV InfectionsGut floraPathology and Laboratory MedicineSystemic inflammationlaw.inventionPlacebosProbiotic0302 clinical medicineImmunodeficiency ViruseslawMedicine and Health SciencesMedicinelcsh:ScienceImmune ResponseMultidisciplinarybiologyMicrobiotaGenomicsMiddle AgedProbiòticsBacterial PathogensIntestinesSaccharomyces boulardiiMedical MicrobiologyViral PathogensVirusesFemale030211 gastroenterology & hepatologyPathogensmedicine.symptomResearch ArticleSaccharomyces boulardiiAdultImmunologyMicrobial GenomicsMicrobiologySaccharomyces03 medical and health sciencesSigns and SymptomsImmune systemDouble-Blind MethodDiagnostic MedicineRetrovirusesGeneticsVIH (Virus)HumansMicrobiomeMicrobial PathogensInflammationClostridiumBacteriabusiness.industryProbioticsGut BacteriaLentivirusLachnospiraceaelcsh:ROrganismsFungiBiology and Life SciencesHIVbiology.organism_classificationYeast030104 developmental biologyImmunologylcsh:QMicrobiomebusinessPLoS ONE
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