Search results for "LAMP"

showing 10 items of 457 documents

Minor hepatic resection for hepatocellular carcinoma in cirrhotic patients: Kelly clamp crushing resection versus heat coagulative necrosis with bipo…

2011

Hemorrhage and postoperative liver insufficiency are frequent and serious complications of hepatic resection in cirrhotic patients. The aim of this study was to assess retrospectively whether the surgical techniques using Kelly clamp crushing resection or heat coagulative necrosis with a bipolar radiofrequency device can reduce the incidence of the above complications and an eventual recurrence of neoplasia on the liver slice. We retrospectively reviewed the results of 35 patients who had undergone resection for monofocal hepatocellular carcinoma at our center. Thirteen patients (Group A) had undergone liver resection with Kelly clamp crushing resection, 22 patients (Group B) had had liver…

Malemedicine.medical_specialtyNecrosisCarcinoma HepatocellularTime Factorsmedicine.medical_treatmentBlood Loss SurgicalGastroenterologyNecrosisLaparotomyInternal medicinemedicineCarcinomaHepatectomyHumansHCC Liver Cirrhosis Resection Coagulative necrosis Bipolar radiofrequency deviceAgedRetrospective StudiesLaparotomybusiness.industryLiver NeoplasmsRetrospective cohort studyGeneral MedicineEquipment Designmedicine.diseaseSurgical InstrumentsHemostasis SurgicalSurgerySettore MED/18 - Chirurgia GeneraleClampCoagulative necrosisTreatment OutcomeLiverHemostasisHepatocellular carcinomaCatheter AblationFemalemedicine.symptombusinessFollow-Up Studies
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Cardiac effects of isoliquiritigenin

1997

The effects of isoliquiritigenin on force of contraction (Fc), L-type Ca2+ current (I(Ca)) and intracellular Ca2+ concentration ([Ca2+]i) were investigated in rat ventricular heart muscle. Isoliquiritigenin increased Fc and I(Ca) and, after longer exposure times, resting tension and [Ca2+]i. The effect of isoliquiritigenin (100 microM) on I(Ca) was diminished by Rp-cAMPS (30 microM). 1H-[1,2,4]oxa- diazolo[4,3-a]quinoxalin-1-one (50 microM) did not influence the effects of isoliquiritigenin on Fc and I(Ca). The positive inotropic effects of isoprenaline and forskolin, but not of 3-isobutyl-1-methylxanthine, were potentiated by isoliquiritigenin (100 microM). In the presence of milrinone (10…

Malemedicine.medical_specialtyPatch-Clamp TechniquesFura-2In Vitro TechniquesMembrane PotentialsRats Sprague-Dawleychemistry.chemical_compoundChalconeChalconesAldehyde ReductaseInternal medicineIsoprenalinemedicineAnimalsDrug InteractionsEnzyme InhibitorsCyclic GMPPharmacologyPlants MedicinalForskolinMyocardiumPhosphodiesteraseHeartCyclic AMP-Dependent Protein KinasesMyocardial ContractionRatsElectrophysiologyEndocrinologychemistryGuanylate CyclaseMilrinoneCalciumFemalemedicine.symptomSoluble guanylyl cyclaseIsoliquiritigeninMuscle contractionmedicine.drugEuropean Journal of Pharmacology
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Oxytocin Regulates Neurosteroid Modulation of GABAAReceptors in Supraoptic Nucleus around Parturition

2003

In this study, we investigate how neurosteroid sensitivity of GABAAreceptors (GABAARs) is regulated. We examined this issue in neurons of the supraoptic nucleus (SON) of the rat and found that, during parturition, the GABAARs become insensitive to the neurosteroid allopregnanolone attributable to a shift in the balance between the activities of endogenous Ser/Thr phosphatase and PKC. In particular, a constitutive endogenous tone of oxytocin within the SON after parturition suppressed neurosteroid sensitivity of GABAARs via activation of PKC. Vice versa before parturition, during late pregnancy, application of exogenous oxytocin brings the GABAARs from a neurosteroid-sensitive mode toward a …

Malemedicine.medical_specialtyPatch-Clamp TechniquesNeuroactive steroidXenopusMice TransgenicPregnanoloneKidneyLigandsOxytocinTransfectionArticlegamma-Aminobutyric acidSupraoptic nucleusCell LineMicechemistry.chemical_compoundPregnancyInternal medicinemental disordersPhosphoprotein Phosphatasespolycyclic compoundsmedicineAnimalsHumansRats WistarProtein Kinase Cgamma-Aminobutyric AcidMice KnockoutPregnanoloneGABAA receptorGeneral NeuroscienceAllopregnanoloneKidney metabolismBridged Bicyclo Compounds HeterocyclicReceptors GABA-ARatsEndocrinologyAnimals Newbornnervous systemOxytocinchemistryOocytesFemaleSteroidsSupraoptic Nucleushormones hormone substitutes and hormone antagonistsmedicine.drugThe Journal of Neuroscience
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Inhibition by Fendiline of the Transient Outward Current in Rat Ventricular Cardiomyocytes

1999

The effects of fendiline on the transient outward current (Ito) were investigated in rat ventricular cardiomyocytes. Extracellularly applied fendiline reduced peak and steady-state current amplitude of Ito; the inactivation of Ito was accelerated by the drug, which reflects onset of block. The described effects were concentration dependent: half-maximal effects were achieved at approximately 3 microM fendiline. Intracellularly applied fendiline (3 microM) did not affect Ito within 5 min. The steady-state current amplitude of Ito was more efficiently suppressed by the drug at 22 +/- 1 degrees C than at 36 +/- 1 degrees C. The recovery of Ito was analyzed by the application of twin depolarizi…

Malemedicine.medical_specialtyPatch-Clamp TechniquesPotassium ChannelsHeart Ventriclesmedicine.medical_treatmentIn Vitro TechniquesAntiarrhythmic agentdigestive systemMembrane PotentialsRats Sprague-DawleyInternal medicinemedicineAnimalsMyocytePharmacologyCardiac transient outward potassium currentBinding SitesFendilineDose-Response Relationship DrugPulse (signal processing)ChemistryTime constantDepolarizationElectric StimulationRatsIon ExchangeElectrophysiologyEndocrinologyFendilineBiophysicsFemaleCalcium ChannelsCardiology and Cardiovascular MedicineJournal of Cardiovascular Pharmacology
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Mechanism of Block by 4-Aminopyridine of the Transient Outward Current in Rat Ventricular Cardiomyocytes

1998

The effects of 4-aminopyridine (4-AP) on the transient outward current (I to ) were investigated in rat ventricular cardiomyocytes at different values of intracellular pH (pH i ) and extracellular pH (pH o ). The 4-AP was administered either extracellularly (bath application) or intracellularly (diffusion from the intrapipette solution). The 4-AP diminished I to given either from inside or outside the cell membrane. The block by extracellularly applied 4-AP (4-AP o ) of the peak amplitude of I to was decreased by external acidification but increased by external alkalinization: conversely. the block by 4-AP o was decreased by internal alkalinization but increased by internal acidification. I…

Malemedicine.medical_specialtyPatch-Clamp TechniquesTertiary amineHeart VentriclesIntracellular pHIn Vitro TechniquesIon ChannelsMembrane PotentialsRats Sprague-DawleyCell membraneInternal medicinemedicineExtracellularAnimalsMyocyte4-AminopyridinePharmacologyCardiac transient outward potassium currentChemistryMyocardiumCell Membrane4-AminopyridineHydrogen-Ion ConcentrationRatsEndocrinologymedicine.anatomical_structureBiophysicsFemaleExtracellular SpaceCardiology and Cardiovascular MedicineIntracellularmedicine.drugJournal of Cardiovascular Pharmacology
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Voltage-Dependent Effects of Barnidipine in Rat Vascular Smooth Muscle

2003

The effects of the dihydropyridine nifedipine and its more lipophilic congener, barnidipine, were investigated in smooth muscle preparations from the rat in resting and depolarizing conditions. Both drugs relaxed precontracted aortic rings more potently in depolarizing conditions, barnidipine being more potent than nifedipine. Currents through Ca 2+ channels in rat vascular smooth muscle cells (A7r5) and in isolated rat cardiomyocytes were reduced more potently by both drugs at a holding potential of-40 mV than at -80 mV. However, barnidipine and nifedipine were more effective in reducing the current in A7r5 cells than in cardiomyocytes. The IC 50 obtained in aortic rings and in A7r5 cells …

Malemedicine.medical_specialtyPatch-Clamp TechniquesVascular smooth muscleBarnidipineNifedipinechemistry.chemical_elementPharmacologyCalciumMuscle Smooth VascularRats Sprague-DawleyNifedipineInternal medicinemedicineAnimalsMyocyteCells CulturedPharmacologyChemistryDihydropyridineDepolarizationCalcium Channel BlockersRatsEndocrinologyMechanism of actioncardiovascular systemFemalemedicine.symptomCardiology and Cardiovascular Medicinemedicine.drugJournal of Cardiovascular Pharmacology
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Depolarization-induced influx of sodium in response to phenylephrine in rat atrial heart muscle.

1991

1. The effects of alpha 1-adrenoceptor stimulation on transmembrane potential, currents and ion fluxes were investigated in multicellular preparations and/or single cells obtained from the left atrium of rat hearts. 2. In multicellular preparations, phenylephrine caused a concentration-dependent positive inotropic effect, an increase in action potential duration, and a decrease in resting potential; the effects were antagonized by phentolamine. 3. In the presence of phenylephrine (100 mumol/1), two levels of resting potential were observed when the preparations were, alternately, electrically stimulated or kept at rest (-74 +/- 1 mV during activity and -62 +/- 4 mV at rest; mean +/- S.E.M.;…

Malemedicine.medical_specialtyPhysiologyVoltage clampAction PotentialsStimulationMembrane PotentialsPhenylephrinechemistry.chemical_compoundPhentolamineInternal medicinemedicineAnimalsHeart AtriaPhenylephrineMembrane potentialChemistryMyocardiumSodiumRats Inbred StrainsDepolarizationMyocardial ContractionResting potentialStimulation ChemicalRatsElectrophysiologyEndocrinologyTetrodotoxinCalciumResearch Articlemedicine.drugThe Journal of Physiology
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No evidence of androgenic hormone from the testes of the glowworm, Lampyris noctiluca.

2005

The widely accepted concept, stating that insects have no true sex hormones, and that primary as well as secondary sex characteristics are controlled by the genetic inventory of each single cell, is challenged by the report of Naisse, J. [1966a. Controle endocrinien de la differenciation sexuelle chez l'Insecte Lampyris noctiluca (Coleoptere Malacoderme Lampyride). I. Role androgene des testicules, Arch. Biol. Liege, 77, 139-201] on the discovery of an androgenic hormone in the glowworm, Lampyris noctiluca. This case is of special interest, since it may point to an ancestral mode of sex differentiation in arthropods, considering that androgenic hormones have been discovered and characterize…

Malemedicine.medical_specialtySex Differentiationmedicine.drug_classSecondary sex characteristicPhysiologyOvaryEndocrinologyInternal medicineTestismedicineAnimalsGlowwormSexual differentiationbiologyOvaryAndrogenbiology.organism_classificationTransplantationColeopteramedicine.anatomical_structureEndocrinologyLarvaAndrogensLampyris noctilucaAnimal Science and ZoologyFemaleVitellogenesisGeneral and comparative endocrinology
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A minimally invasive approach for aortobifemoral bypass procedure

2011

Surgical aortobifemoral bypass procedure for aortoiliac occlusive disease remains the gold standard treatment despite rapidly expanding range of indications for endovascular repair. Besides several disadvantages such as dysparaesthesias, hernias, and unpleasant outcome, transperitoneal exposure of the aorta is also associated with operative autonomic nerve injury. In five male patients, infrarenal aorta was exposed through a small (8 cm) supraumbilical midline incision. Incision of the posterior peritoneum above the infrarenal aorta was limited to 3 cm. A 1 cm infraumbilical incision allowed transperitoneal placement of the distal aortic clamp outside of the operative field. Four centimeter…

Malemedicine.medical_specialtyTime FactorsAortic DiseasesAortoiliac occlusive diseaseArterial Occlusive DiseasesConstriction PathologicAnastomosisAortographyIliac ArteryBlood Vessel Prosthesis ImplantationPostoperative Complicationsmedicine.arterymedicineHospital dischargeHumansMinimally Invasive Surgical ProceduresAortaAgedAortaCentimeterbusiness.industryLength of StayMiddle Agedmedicine.diseaseConstrictionPatient DischargeSurgeryFemoral ArteryClampTreatment OutcomeAnesthesiaSurgeryCardiology and Cardiovascular MedicinebusinessTomography X-Ray ComputedIntermediate careMagnetic Resonance AngiographyAbdominal surgeryJournal of Vascular Surgery
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ANATOMICAL AND FUNCTIONAL OUTCOMES OF SYMPTOMATIC IDIOPATHIC VITREOMACULAR TRACTION

2016

To describe the natural history of eyes with symptomatic idiopathic vitreomacular traction (VMT).Retrospective multicenter study of 168 eyes with spectral-domain optical coherence tomography (SD-OCT) findings consistent with idiopathic VMT. All eyes were graded according to SD-OCT findings. Grade 1 was defined as incomplete cortical vitreous separation with foveal attachment. Grade 2 was defined as Grade 1 plus intraretinal cysts or clefts. Grade 3 was defined as Grade 2 plus a foveal detachment. All patients were followed for at least 6 months.There were 168 patients (51 men) with a mean age of 68.8 ± 10.7 years. Patients were followed for a mean of 22.7 ± 20.1 months. The mean duration of…

Malemedicine.medical_specialtyVisual acuitygenetic structuresRemission SpontaneousVisual AcuityTissue AdhesionsVitreomacular tractionVitreous Detachment03 medical and health scienceschemistry.chemical_compound0302 clinical medicineFibrinolytic AgentsRetinal DiseasesVitrectomyOphthalmologymedicineHumansMedical historyFibrinolysin030212 general & internal medicineMacular holeAgedRetrospective StudiesSlit Lampbusiness.industryOcriplasminGeneral MedicineRetinal Perforationsmedicine.diseasePeptide Fragmentseye diseasesSurgeryNatural historyOphthalmologychemistry030221 ophthalmology & optometryFemalesense organsmedicine.symptombusinessTomography Optical CoherenceNatural history studyFibrinolytic agentRetina
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