Search results for "LET"

The Nasal Epithelium as a Factory for Systemic Protein Delivery

2002

We have previously shown that recombinant Sendai virus (SeV) produces efficient in vivo airway epithelial gene transfer. The ability to produce therapeutic levels of circulating proteins following noninvasive gene transfer would have widespread clinical application. Here, we compared nose, lung, and skeletal muscle for the ability to produce circulating levels of the secreted mouse antiinflammatory cytokine interleukin-10 (IL10) following SeV-mediated gene transfer. High levels of serum IL10 were obtained from each site with a potency order of lung > nose > muscle for a given viral titer. Serum levels from each site were within the likely required range for anti-inflammatory effects. The co…

In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor.

1996

AbstractProteolytic processing of the nonstructural proteins of the hepatitis C virus (HCV) is mediated by two viral proteinases: the NS2-3 proteinase cleaving at the NS2/3 junction and the NS3 serine-type proteinase responsible for processing at the NS3/4A, NS4A/B, NS4B/5A, and NS5A/B sites. Activity of the NS3 proteinase is modulated by NS4A. In the absence of this cofactor processing at the NS3-dependent sites does not occur or, in the case of the NS5A/B junction, is poor but increased when NS4A is present. Although recent studies demonstrated that proteinase activation requires direct interaction between NS3 and NS4A, the mechanism by which NS4A exerts the activation function is not kno…

PTC124-mediated translational readthrough of a nonsense mutation causing Usher syndrome type 1C.

2011

We investigated the therapeutic potential of the premature termination codon (PTC) readthrough-inducing drug PTC124 in treating the retinal phenotype of Usher syndrome, caused by a nonsense mutation in the USH1C gene. Applications in cell culture, organotypic retina cultures, and mice in vivo revealed significant readthrough and the recovery of protein function. In comparison with other readthrough drugs, namely the clinically approved readthrough-inducing aminoglycoside gentamicin, PTC124 exhibits significant better retinal biocompatibility. Its high readthrough efficiency in combination with excellent biocompatibility makes PTC124 a promising therapeutic agent for PTCs in USH1C, as well a…

Clathrin- and Caveolin-Independent Entry of Human Papillomavirus Type 16—Involvement of Tetraspanin-Enriched Microdomains (TEMs)

2008

BACKGROUND: Infectious entry of human papillomaviruses into their host cells is an important step in the viral life cycle. For cell binding these viruses use proteoglycans as initial attachment sites. Subsequent transfer to a secondary receptor molecule seems to be involved in virus uptake. Depending on the papillomavirus subtype, it has been reported that entry occurs by clathrin- or caveolin-mediated mechanisms. Regarding human papillomavirus type 16 (HPV16), the primary etiologic agent for development of cervical cancer, clathrin-mediated endocytosis was described as infectious entry pathway. METHODOLOGY/PRINCIPAL FINDINGS: Using immunofluorescence and infection studies we show in contra…

Ultrasound-guided viscosupplementation in glenohumeral microinstability in sports athletes

2014

Cultura visuale. Una genealogia

2020

Questo libro offre una ricognizione delle principali genealogie novecentesche della cultura visuale, scegliendo solo tre figure paradigmatiche: Aby Warburg, Sigmund Freud e Walter Benjamin. Dopo Warburg la nozione di immagine è definitivamente cambiata. Dopo Freud lo sguardo è indissolubilmente legato al tema della sessualità e del bios. Dopo Benjamin la nozione di dispositivo e quelle di apparato, assemblage e ambiente mediale sono definitivamente cambiate. Questi autori non stanno solo alle origini della cultura visuale ma ne prefigurano gli sviluppi futuri. Da Warburg abbiamo imparato che il fare-immagine fa parte della biologia e dei comportamenti essenziali dell’Homo sapiens, un aspett…

A Moldvai Csángómagyar Oktatási Program kutatása és támogatása vizuális módszerekkel

2018

A Moldvai Csángómagyar Oktatási Program kutatása és támogatása egy több éves (2016– 2021), a Finn Akadémia által támogatott projekt (projektszáma, 299133), amelynek anyaga 30 moldvai településen folyatatott, 2017–2019 között végzett terepmunkából áll. A terepmunka szakaszai során számos vizuális és digitális módszertani eljárás is bevezetésre kerül, s az ezek segítségével készített anyagokból nagy terjedelmű, multimodális adatbázis jön létre. Cikkemben néhány, eddig sikeresnek bizonyult módszert mutatok be, különös tekintettel arra, hogy ezek segítségével hogyan válaszolhatók meg a kutatási kérdéseim. A kötet témájához igazodva kutatásomnak a nyelvitájkép-kutatások között elfoglalt helyét i…

Lessico della cultura dialettale delle Madonie. 2. Voci di saggio

2011

Nella prospettiva di una sistematica ricognizione del patrimonio lessicale di un'area di grande interesse etnodialettale come quella madonita e dopo il volume sulla cultura alimentare tradizionale, il volume presenta oltre 3000 voci di saggio e numerose unità fraseologiche relative alla terminolgia connessa ai settori della cultura tradizionale. Le voci si riferiscono, in particolare, ai più significativi concetti dell'agricoltura e della pastorizia, ma presentano anche molti ambiti del mondo "naturale" (zoonimi, fitonimi, miconimi, meteoronimi). Il volume è corredato di carte linguistiche a colori sui principali tratti della cultura dialettale delle Madonie; il libro è arricchito da numero…

Recensione di: Vida y muerte en el Corpus Hippocraticum, di M. T. Gallego Pérez

2020

Recensione di un volume dedicato all'indagine dei campi lessicali e semantici dei concetti di 'vita' e 'morte' negli scritti del Corpus ippocratico.

Learning and teaching oral communication in English in vocational school : learners' and teachers' views

2015

Tutkimuksen tarkoituksena oli selvittää Englannin opetuksen tilaa ammatillisessa koulutuksessa. Suullista kielitaitoa painotetaan Ammatillisen perustutkinnon perusteissa sekä koulukohtaisissa opetussuunnitelmissa, joten myös tämä tutkimus keskittyi siihen. Vastaavia tutkimuksia on aiemmin tehty lukio-opetuksesta, mutta ei ammatillisesta opetuksesta. Aihe on tärkeä koska Suomessa osallistuu vuosittain satoja tuhansia ihmisiä ammatilliseen koulutukseen. Tutkimukseen osallistui 61 kolmannen vuoden opiskelijaa ja kolme opettajaa. Oli tärkeää, että molempien osapuolten näkemykset pääsivät esiin. Opiskelijoilta data kerättiin Internet-kyselyn avulla, jossa oli sekä monivalintakysymyksiä että avoi…