Search results for "LINE-1"
showing 10 items of 43 documents
DNA methylation changes and somatic mutations as tumorigenic events in Lynch syndrome-associated adenomas retaining mismatch repair protein expression
2018
Background: DNA mismatch repair (MMR) defects are a major factor in colorectal tumorigenesis in Lynch syndrome (LS) and 15% of sporadic cases. Some adenomas from carriers of inherited MMR gene mutations have intact MMR protein expression implying other mechanisms accelerating tumorigenesis. We determined roles of DNA methylation changes and somatic mutations in cancer-associated genes as tumorigenic events in LS-associated colorectal adenomas with intact MMR. Methods: We investigated 122 archival colorectal specimens of normal mucosae, adenomas and carcinomas from 57 LS patients. MMR-deficient (MMR-D, n 49) and MMR-proficient (MMR-P, n 18) adenomas were of particular interest and were inter…
CCDC 1569164: Experimental Crystal Structure Determination
2017
Related Article: Toms Rekis, Simone d’Agostino, Dario Braga, Fabrizia Grepioni|2017|Cryst.Growth Des.|17|6477|doi:10.1021/acs.cgd.7b01146
CCDC 1569165: Experimental Crystal Structure Determination
2017
Related Article: Toms Rekis, Simone d’Agostino, Dario Braga, Fabrizia Grepioni|2017|Cryst.Growth Des.|17|6477|doi:10.1021/acs.cgd.7b01146
CCDC 1569162: Experimental Crystal Structure Determination
2017
Related Article: Toms Rekis, Simone d’Agostino, Dario Braga, Fabrizia Grepioni|2017|Cryst.Growth Des.|17|6477|doi:10.1021/acs.cgd.7b01146
CCDC 199926: Experimental Crystal Structure Determination
2003
Related Article: E.Coronado, C.Gimenez-Saiz, M.Nicolas, F.M.Romero, E.Rusanov, H.Stoeckli-Evans|2003|New J.Chem.|27|490|doi:10.1039/b208762f
CCDC 2070166: Experimental Crystal Structure Determination
2021
Related Article: Shrabani Saha, Sujoy Das, Olivia Sarkar, Ansuman Chattopadhyay, Kari Rissanen, Prithidipa Sahoo|2021|New J.Chem.|45|17095|doi:10.1039/D1NJ02661E
Coordinate mutation and transformation of mouse fibroblasts: induction by nitroquinoline oxide and modulation by caffeine
1981
Mutation and malignant transformation were followed in the same cells. Mouse fibroblasts (C3H 10T 1/2) were mutated and transformed by 4-nitroquinoline-1-oxide with similar, approximately linear dose-responses. The presence of caffeine immediately after exposure to 4-nitroquinoline-1-oxide potently inhibited mutation and transformation at high but not at low doses of 4-nitroquinoline-1-oxide. Whilst the coordinate induction of mutation and transformation could be explained by both a common target (DNA) or a common reactive species hitting several targets, the identical modulation by a DNA repair inhibitor of both end points suggests fundamental similarities in the nature of the lesions lead…
Use of HepG2 cell line for direct or indirect mutagens screening: comparative investigation between comet and micronucleus assays.
2003
International audience; In the present study, DNA-damage and clastogenic or aneugenic effects of genotoxic compounds were examined in a metabolically competent human cell line (HepG2 cells) using the micronucleus and the comet assays. Compounds with various action mechanisms were tested: direct mutagens such as 4-nitroquinoline-N-oxide (4-NQO) and methyl methanesulfonate (MMS) and indirect mutagens requiring biotransformation to be active such as N-nitrosodimethylamine (NDMA), benzo[a]pyrene (B[a]P) and 2-acetylaminofluorene (2-AAF). The compounds were first tested for cytotoxicity by measuring their effects on RNA synthesis inhibition in HepG2 cells. 4-NQO, B[a]P and 2-AAF were the most po…
On the relevance of genotoxicity for fish populations II: genotoxic effects in zebrafish (Danio rerio) exposed to 4-nitroquinoline-1-oxide in a compl…
2003
In order to characterize the impact of genotoxic potentials on populations of aquatic organisms in surface waters, zebrafish (Danio rerio) were exposed to the model genotoxicant 4-nitroquinoline-1-oxide (NQO) in a complete life-cycle test. Fish exposed to mean NQO concentrations of 0, 0.1, 0.3, 1.1, and 2.9 microg/l were examined by several genotoxicity assays with different endpoints. Assays included the unscheduled DNA synthesis (UDS) test, the comet assay, the alkaline filter elution, and the micronucleus test. The genotoxicity assays revealed an increasing genotoxicity, ranging from induction of DNA repair (even at the lowest concentration tested) to primary and secondary DNA alteration…
Detection of primary DNA damage in Chlamydomonas reinhardtii by means of modified microgel electrophoresis.
1997
The assessment of genotoxic potential in surface water requires test methods, among which are those that detect initial DNA damage in organisms of aquatic biocenosis. The microgel electrophoresis (MGE) "comet assay" was applied to a ubiquitous unicellular green alga (Chlamydomonas reinhardtii) to detect DNA damage caused by genotoxins. For this, the test protocol described by Singh NP et al. [Exp Cell Res 175: 184-191, 1988] was modified. Major modifications were the use of alkaline lysis buffer with ionic detergents and the reduction of preincubation and electrophoresis times. Short-time exposure of Chlamydomonas to the well-known genotoxicants 4-nitroquinoline-1-oxide (4-NQO), N-nitrosodi…