Search results for "LIX"

Transcriptional regulation of the stem cell leukemia gene by PU.1 and Elf-1.

1998

Abstract The SCL gene, also known astal-1, encodes a basic helix-loop-helix transcription factor that is pivotal for the normal development of all hematopoietic lineages. SCL is expressed in committed erythroid, mast, and megakaryocytic cells as well as in hematopoietic stem cells. Nothing is known about the regulation of SCL transcription in mast cells, and in other lineages GATA-1 is the only tissue-specific transcription factor recognized to regulate the SCL gene. We have therefore analyzed the molecular mechanisms underlyingSCL expression in mast cells. In this paper, we demonstrate that SCL promoter 1a was regulated by GATA-1 together with Sp1 and Sp3 in a manner similar to the situati…

Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes

2015

Summary Direct lineage reprogramming induces dramatic shifts in cellular identity, employing poorly understood mechanisms. Recently, we demonstrated that expression of Neurog2 or Ascl1 in postnatal mouse astrocytes generates glutamatergic or GABAergic neurons. Here, we take advantage of this model to study dynamics of neuronal cell fate acquisition at the transcriptional level. We found that Neurog2 and Ascl1 rapidly elicited distinct neurogenic programs with only a small subset of shared target genes. Within this subset, only NeuroD4 could by itself induce neuronal reprogramming in both mouse and human astrocytes, while co-expression with Insm1 was required for glutamatergic maturation. Cu…

Recruitment of HIF-1α and HIF-2α to common target genes is differentially regulated in neuroblastoma: HIF-2α promotes an aggressive phenotype

2006

In neuroblastoma specimens, HIF-2alpha but not HIF-1alpha is strongly expressed in well-vascularized areas. In vitro, HIF-2alpha protein was stabilized at 5% O2 (resembling end capillary oxygen conditions) and, in contrast to the low HIF-1alpha activity at this oxygen level, actively transcribed genes like VEGF. Under hypoxia (1% O2), HIF-1alpha was transiently stabilized and primarily mediated acute responses, whereas HIF-2alpha protein gradually accumulated and governed prolonged hypoxic gene activation. Knockdown of HIF-2alpha reduced growth of neuroblastoma tumors in athymic mice. Furthermore, high HIF-2alpha protein levels were correlated with advanced clinical stage and high VEGF expr…

Fertility and Polarized Cell Growth Depends on eIF5A for Translation of Polyproline-Rich Formins in Saccharomyces cerevisiae

2014

eIF5A is an essential and evolutionary conserved translation elongation factor, which has recently been proposed to be required for the translation of proteins with consecutive prolines. The binding of eIF5A to ribosomes occurs upon its activation by hypusination, a modification that requires spermidine, an essential factor for mammalian fertility that also promotes yeast mating. We show that in response to pheromone, hypusinated eIF5A is required for shmoo formation, localization of polarisome components, induction of cell fusion proteins, and actin assembly in yeast. We also show that eIF5A is required for the translation of Bni1, a proline-rich formin involved in polarized growth during …

Interaction of the Alternating Double Stranded Copolymer poly(dA-dT)·poly(dA-dT) with NiCl2and CdCl22: Solution Behavior

2007

The thermal denaturation of the synthetic high molecular weight double stranded polynucleotide poly(dA-dT) x poly(dA-dT) has been studied in aqueous buffered solution (Tris 1.0 mM; pH 7.8+/-0.2) in the presence of increasing concentrations of either Ni(2+) (borderline cation) or Cd(2+) (soft cation) at four different constant ionic strength values (NaCl), making use of UV and circular dichroism (CD) spectroscopies. The experimental results show that the B-type double helix of the polymer is stabilized against thermal denaturation in the presence of both cations at low concentrations, relative to the systems where only NaCl is present, in the same conditions of ionic strength and pH. The eff…

Selective dimerisation of tetraurea calix[4]arenes

2010

The formation of hydrogen-bonded dimeric capsules from tetraurea calix[4]arenes is controlled by loops, connecting adjacent urea functions, and more or less bulky substituents. The dimerisation is only possible if loops are not overlapping and if the respective residues can pass the loops. A sorting scheme based on small and bulky residues and one to four loops allows reducing the number of possible dimers from 35 to 6 in a stoichiometric mixture of 11 ureas. With three different loop sizes (O–(CH2) n –O chains with n = 10, 14, 20 connecting adjacent phenylurea functions via their meta positions), it is possible to distinguish four urea residues of different sizes (small, medium, bulky and …

Infliximab and topical tacrolimus administration effective in the treatment of Pyoderma Gangrenosum.

2009

Alix protein is substrate of Ozz-E3 ligase and modulates actin remodeling in skeletal muscle

2012

Alix/AIP1 is a multifunctional adaptor protein that participates in basic cellular processes, including membrane trafficking and actin cytoskeleton assembly, by binding selectively to a variety of partner proteins. However, the mechanisms regulating Alix turnover, subcellular distribution, and function in muscle cells are unknown. We now report that Alix is expressed in skeletal muscle throughout myogenic differentiation. In myotubes, a specific pool of Alix colocalizes with Ozz, the substrate-binding component of the muscle-specific ubiquitin ligase complex Ozz-E3. We found that interaction of the two endogenous proteins in the differentiated muscle fibers changes Alix conformation and pro…

Hectoris spolium. (Sen. Troad. 990). Una controversa rappresentazione di Ecuba nelle Troiane di Seneca

2020

In the invective that Hecuba addresses to Ulysses in Troad. 990-991 Leo was the first to judge the expression Hectoris spolium feret / qui tulit Achillis? interpolated. Retracing the history of the interpretation, the paper considers the verses authentic: they offer a brilliant line of the queen, who ironically defines herself as Hectoris spolium.

Uzņēmuma vērtības vadīšanas pilnveidošana multimediju straumēšanas nozarē.

2019

Ar uzņēmuma finansiālajiem rādītājiem ir iespējams noteikt cik efektīvi uzņēmums nodarbojas ar saimniecisko darbību. Vadībai pieņemot tālākās attīstības stratēģijas ir nepieciešams ņemt tos vērā un lēmumu balstīt uz tiem. Vērtības rādītājs ir lielums, kas aptver daudzus finansiālos faktorus vienkopus. Pētījums ir veikts, lai izprastu vērtības nozīmību strauji augošajā multimediju straumēšanas nozarē, kur ir raksturīgas augstas uzņēmumu tirgus vērtības pret uzņēmumu aktīvu daudzumu un ienākumiem. Pētījumā tiek veikta uzņēmumu vērtību detalizācija, lai izprastu tiešo vērtības nozīmi un tās ietekmējošos faktorus. Pētījuma rezultātā tiek iegūtas uzņēmumu vērtības, kas tiek aprēķinātas pēc vairā…