Search results for "LMMV"

showing 3 items of 3 documents

The complete genome sequence of Lamium mild mosaic virus, a member of the genus Fabavirus

2013

Springer-Verlag Wien 2013 Abstract Lamium mild mosaic virus (LMMV) is the only one of the five members of the genus Fabavirus for which there are no nucleotide sequence data. In this study, the complete genome sequence of LMMV was determined and compared with the available complete genome sequences of other members of the genus Fabavirus. The genome was the largest of the genus but maintained the typical orga- nization, with RNA 1 of 6080 nucleotides (nt), RNA 2 of 4065 nt, and an unusually long 3 0 untranslated region in RNA 2 of 603 nt. Phylogenetic analysis of the amino acid sequences of the protease-polymerase (Pro-Pol) region and the two coat proteins confirmed that LMMV belongs to a d…

SubfamilyLMMVMolecular Sequence DataGenome ViralGenomeFabaviruBroad bean wilt virusViral ProteinsSpecies SpecificityGenusMosaic VirusesVirologySecoviridaeTobaccoComovirinaePhylogenyPlant DiseasesGeneticsWhole genome sequencingLamiaceaebiologyBase SequenceNucleic acid sequenceSettore AGR/12 - Patologia Vegetalefood and beveragesGeneral MedicineDNA-Directed RNA PolymerasesSequence Analysis DNAClassificationbiology.organism_classificationVirologyFabavirusRNA ViralCapsid ProteinsPeptide Hydrolases
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Detection and identification Fabavirus species by one-step RT-PCR and multiplex RT-PCR

2014

The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV). The development is described of two RT-PCR procedures for the detection and identification of Fabavirus species: a one-step RT-PCR using a single pair of conserved primers for the detection of all fabaviruses, and a one-step multiplex RT-PCR using species-specific primers for the simultaneous detection and identification of the above-mentioned species of the genus Fabavirus. These m…

Time FactorsbiologyReverse Transcriptase Polymerase Chain ReactionBBWV-1 BBWV-2 GeMV CuMMV LMMV detection diagnosis multiplex RT-PCR conserved primersLamium mild mosaic virusSettore AGR/12 - Patologia VegetaleDetection Diagnosis Conserved primersbiology.organism_classificationSensitivity and SpecificityVirologyFabavirusBroad bean wilt virusReal-time polymerase chain reactionGenusVirologyPlant virusSecoviridaeRNA ViralIdentification (biology)MultiplexDNA PrimersPlant Diseases
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Rapid detection and discrimination of fabaviruses by flow-through hybridisation with genus- and species-specific riboprobes

2015

Viruses cause significant damage in agricultural crops worldwide. Disease management requires sensitive and specific tools for virus detection and identification. Also, detection techniques need to be rapid to keep pace with the continuous emergence of new viral diseases. The genus Fabavirus is composed of five viruses infecting many economically important crops worldwide. This research describes the development of a procedure based on flow-through hybridisation (FTH), which is faster than and as sensitive as conventional hybridisation for virus detection in tissue-prints from infected plants. Six digoxigenin-labelled RNA probes were synthesised with two levels of specificity: (a) five spec…

virology.FabaviruGeMVmicrobial forensicSecoviridaeBWV-1LMMVplant virologySettore AGR/12 - Patologia VegetaleBBWV-2biosecurityCuMMV
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