Search results for "LOGS"

Synthetic mRNAs with Superior Translation and Stability Properties

2012

The translational efficiency and stability of synthetic mRNA in both cultured cells and whole animals can be improved by incorporation of modified cap structures at the 5'-end. mRNAs are synthesized in vitro by a phage RNA polymerase transcribing a plasmid containing the mRNA sequence in the presence of all four NTPs plus a cap dinucleotide. Modifications in the cap dinucleotide at the 2'- or 3'-positions of m(7)Guo, or modifications in the polyphosphate chain, can improve both translational efficiency and stability of the mRNA, thereby increasing the amount and duration of protein expression. In the context of RNA-based immunotherapy, the latter is especially important for antigen producti…

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune r…

2010

Vaccination with in vitro transcribed RNA coding for tumor antigens is considered a promising approach for cancer immunotherapy and has already entered human clinical testing. One of the basic objectives for development of RNA as a drug is the optimization of immunobioavailability of the encoded antigen in vivo. By analyzing the effect of different synthetic 5' mRNA cap analogs on the kinetics of the encoded protein, we found that m(2)(7,2'-O)Gpp(S)pG (beta-S-ARCA) phosphorothioate caps, in particular the D1 diastereoisomer, profoundly enhance RNA stability and translational efficiency in immature but not mature dendritic cells. Moreover, in vivo delivery of the antigen as beta-S-ARCA(D1)-c…

Katalog der Bibliothek des Naturforscher-Vereins zu Riga,1

1908

Inhalt: A. Bibliographie und Biographie ; B. Naturwissenschaftliche Schriften gemischten Inhalts ; C. Vereins- und periodishe Schriften

Administratīvo tiesu dialogs ar Satversmes tiesu

2019

Administratīvās tiesas jau vairāk nekā 14 gadus iesaistās dialogā ar Satversmes tiesu, lai objektīvi un taisnīgi izspriestu tās izskatīšanā esošās lietas, tomēr līdz šim Latvijā minētais tiesu dialogs pētīts maz. Tālab darbā tiek pētīts ar tiesas pieteikuma palīdzību īstenotā administratīvo tiesu dialoga ar Satversmes tiesu veidošanās pamats, galvenās pazīmes, problēmjautājumi un attīstības virzieni, īpašu uzmanību pievēršot administratīvo tiesu kompetencei pašām veikt tiesību normu hierarhijas kontroli. Pētījuma rezultātā secināts, ka tiesu dialogs ir nozīmīgs procesuāls līdzeklis, ar kuru iespējams nodrošināt efektīvu un augstu personu pamattiesību aizsardzības līmeni, veidojot vienotu iz…

Is there any scaling in the cluster distribution?

1994

We apply fractal analysis methods to investigate the scaling properties in the Abell and ACO catalogs of rich galaxy clusters. We also discuss different technical aspects of the method when applied to data sets with small number of points as the cluster catalogs. Results are compared with simulations based on the Zel'dovich approximation. We limit our analysis to scales less than 100 $\hm$. The cluster distribution show a scale invariant multifractal behavior in a limited scale range. For the Abell catalog this range is 15--60$\hm$, while for the ACO sample it extends to smaller scales. Despite this difference in the extension of the scale--range where scale--invariant clustering takes plac…

Latvijas Universitātes tautsaimniecības un tiesību zinātņu fakultātes bibliotēkas sistēmatiskais katalogs, 1. sēj.

1930

Latvijas Universitātes tautsaimniecības un tiesību zinātņu fakultātes bibliotēkas sistēmatiskais katalogs, 2. sēj.

1938

In rete la cucina è politica. Alcuni modelli di analisi e uno studio di caso.

2013

Gastronauti e chowhounds, ghiottoni, gourmand e gourmet, in una parola foodies: l’attuale megatrend culinario deve molto alle pratiche di socializzazione su Internet. Di cibo, in rete, si comincia a scrivere fin da subito (i board di Chowhound, per esempio, aprono i battenti già nel 97), la qual cosa dice anche molto su quanto la “grande conversazione” sia debitrice del discorso gastronomico. Il cibo e le chiacchere su Internet si presentano, infatti, come intrinsecamente legati, classico binomio inscindibile. In tutto il mondo, blogger e storyteller culinari diventano, pertanto, autori di culto, in grado di generare schiere di fedeli lettori pronti a seguire il proprio beniamino ovunque, s…

Inhibition of Cancer Derived Cell Lines Proliferation by Synthesized Hydroxylated Stilbenes and New Ferrocenyl-Stilbene Analogs. Comparison with Resv…

2014

Further advances in understanding the mechanism of action of resveratrol and its application require new analogs to identify the structural determinants for the cell proliferation inhibition potency. Therefore, we synthesized new trans-resveratrol derivatives by using the Wittig and Heck methods, thus modifying the hydroxylation and methoxylation patterns of the parent molecule. Moreover, we also synthesized new ferrocenylstilbene analogs by using an original protective group in the Wittig procedure. By performing cell proliferation assays we observed that the resveratrol derivatives show inhibition on the human colorectal tumor SW480 cell line. On the other hand, cell viability/cytotoxicit…

N-Benzyl Residues as the P1′ Substituents in Phosphorus-Containing Extended Transition State Analog Inhibitors of Metalloaminopeptidases

2020

Peptidyl enzyme inhibitors containing an internal aminomethylphosphinic bond system (P(O)(OH)-CH2-NH) can be termed extended transition state analogs by similarity to the corresponding phosphonamidates (P(O)(OH)-NH). Phosphonamidate pseudopeptides are broadly recognized as competitive mechanism-based inhibitors of metalloenzymes, mainly hydrolases. Their practical use is, however, limited by hydrolytic instability, which is particularly restricting for dipeptide analogs. Extension of phosphonamidates by addition of the methylene group produces a P-C-N system fully resistant in water conditions. In the current work, we present a versatile synthetic approach to such modified dipeptides, based…