Search results for "LR"
showing 10 items of 764 documents
Cutting edge: priming of CTL by transcutaneous peptide immunization with imiquimod.
2005
Abstract CTL are important in combating cancer and viruses. Therefore, triggering the complete potential of CTL effector functions by new vaccination strategies will not only improve prophylaxis of tumor or virus-related diseases, but also open opportunities for effective therapeutic immunizations. Using transcutaneous immunization, we show that epicutaneous (e.c.)4 application of an ointment containing a CTL epitope and the TLR7 ligand imiquimod is highly effective in activating T cells in mice using TCR-transgenic CTL or in wild-type mice. Transcutaneous immunization-activated CTL mount a full-blown immune response against the target epitope characterized by proliferation, cytolytic activ…
Heat shock protein-antigen fusions lose their enhanced immunostimulatory capacity after endotoxin depletion.
2008
Heat shock proteins (HSPs) induce cross-presentation of antigens by dendritic cells (DC) as well as DC maturation. These properties make HSP antigen complexes good candidates to prime CD8 T cell responses against tumor-associated antigens. In this study, we analyzed four different members of the HSP70 family fused to a fragment of ovalbumin (OVA) as a model tumor antigen. E. coli-derived recombinant HSP70-OVA fusion proteins efficiently primed antigen-specific cytotoxic T cells in short-term in vivo immunization assays. Because of concerns that the adjuvant effect of HSPs may be due to endotoxin contamination, we studied this issue in detail. Induction of OVA-specific cytotoxicity was signi…
Activation of the human immune system via toll-like receptors by the oncolytic parvovirus H-1.
2012
This study aimed to investigate the function of toll-like receptors (TLRs) during oncolytic parvovirus H-1 (H-1PV)-induced human immune responses. First, the role of TLRs in the activation of the NFκB transcription factor was characterized; second, the immunologic effects of H-1PV-induced tumor cell lysates (TCL) on human antitumor immune responses were evaluated. A human ex vivo model was used to study immune responses with dendritic cells (DCs). Human embryonic kidney cells (HEK293) transfected to stably express TLRs were used as potential human DC equivalents to further investigate the role of specific TLRs during immune activation. TLR3 and TLR9 were activated by H-1PV infection, which …
Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2
2009
Abstract The three calcium-dependent factors NFATc1, c2, and c3 are expressed in cells of the immune system and play pivotal roles in modulating cellular activation. With regard to NFATc2, it was reported that NFATc2-deficient mice display increased immune responses in several models for infection and allergy in vivo. This led to the assumption that NFATc2 is involved in the maintenance of immune homeostasis. Using the synthetic TLR7 agonist imiquimod as an adjuvant in epicutaneous peptide immunization, we observed that both the inflammatory reaction and the peptide-specific CTL response are severely impaired in NFATc2-deficient mice. Detailed analyses revealed that early production of proi…
NLRP3 controls ATM activation in response to DNA damage
2020
The DNA damage response (DDR) is essential to preserve genomic integrity and acts as a barrier to cancer. The ATM pathway orchestrates the cellular response to DNA double strand breaks (DSBs), and its attenuation is frequent during tumorigenesis. Here, we show that NLRP3, a Pattern Recognition Receptor known for its role in the inflammasome complex formation, interacts with the ATM kinase to control the early phase of DDR, independently of its inflammasome activity. NLRP3 down-regulation in human bronchial epithelial cells impairs ATM pathway activation as shown by an altered ATM substrate phosphorylation profile, and due to impaired p53 activation, confers resistance to acute genomic stres…
A novel tunicate (Botryllus schlosseri) putative C-type lectin features an immunoglobulin domain.
1997
We have cloned a putative C-type lectin of Botryllus schlosseri [Ascidiacea], whose deduced protein of 333 amino acids features three building blocks: (i) a Greek-key motif signature at the amino-terminus, (ii) a C-type lectin domain signature, and (iii) an immunoglobulin (Ig) domain at the carboxyl terminus. This C-type lectin was termed BSCLT. Similarity searches revealed that the Ig domain in BSCLT, which is evidently not polymorphic, is best classified as an Intermediate-type Ig domain. Rabbit antibodies, raised against recombinant BSCLT, cross-reacted in a Western blot with a 38-kD polypeptide in tunicate crude extract. Presumably, this bimodal tunicate protein is the first description…
Capitanes del Renacimiento. Ética militar en la España mediterránea, c. 1500-1550
2004
En la Europa del siglo XVI los valores caballerescos, fundamento de la ética de la aristocracia militar, se enriquecieron con los ideales cortesanos. En este artículo estudiamos la difusión de esos ideales entre los oficiales del ejército de la Monarquía hispánica en los diversos reinos de la España Mediterránea. Estimamos que esta conjunción de principios morales proporcionó la base para una incipiente disciplina de los mandos militares. In Sixteenth Century Europe, the chivalric values which were in the core of military aristocracy ethics became enriched with court-like ideals. In this paper we study the diffusion of those ideals among army officers of the Hispanic Monarchy in the various…
Alaraajojen luun mineraalipitoisuuden ja polven nivelruston väliset yhteydet postmenopausaalisilla naisilla
2011
Tämän poikkileikkaustutkimuksen tarkoituksena oli selvittää luuston (reisiluun kaula, lanneranka L2-L4) mineraalitiheysarvojen ja polven nivelruston (reisiluu, sääriluu) eheyttä kuvaavien dGEMRIC-arvojen välistä yhteyttä postmenopausaalisilla naisilla. Tutkimukseen rekrytoitiin 82 naista (keskimääräiseltä iältään 57,7 vuotta, SD 4,2) lehti-ilmoituksen perusteella. Sisäänottokriteerinä oli rtg:llä arvioidun Kellgren-Lawrence-luokituksen (K/L) mukaan ensimmäisen (K/L1) tai toisen (K/L2) asteen lievä polven nivelrikko. Reisiluun kaulan ja lannerangan (L2–L4) BMC-arvot mitattiin DXA-laitteistolla. Tutkimushenkilöillä ei ollut osteoporoosia, jonka kynnysarvona käytettiin T-arvoa >2,5. Tibian ja …
Relationship between lower limb neuromuscular performance and bone strength in postmenopausal women with mild knee osteoarthritis.
2014
Objectives: To investigate whether neuromuscular performance predicts lower limb bone strength in different lower limb sites in postmenopausal women with mild knee osteoarthritis (OA). Methods: Neuromuscular performance of 139 volunteer women aged 50-68 with mild knee OA was measured using maximal counter movement jump test, isometric knee flexion and extension force and figure-of-eight-running test. Femoral neck section modulus (Z, mm³) was determined by data obtained from dualenergy X-ray absorptiometry. Data obtained using peripheral quantitative computed tomography was used to asses distal tibia compressive (BSId, g2/cm4) and tibial mid-shaft bending (SSImaxmid, mm3) strength indices. R…
Participation Profiles and Socialisation into Dance Among Non‐Professional Dancers
1997
Abstract The study was designed to determine how individuals become socialised into folk, competitive ballroom, ballet and modern dance through an investigation of background variables, the influence of significant others and the participation profiles of dancers, and the identification of those socialization and involvement variables that best discriminate among different groups of dancers. The subjects were 308 adolescent and adult dancers of at least 16 years of age who had pursued their main dance form for a minimum of three years. The data were collected by questionnaire, supplemented with some additional data gathered through personal interviews. Differences between the four dance gro…