Search results for "Lament"

El derecho de los parlamentarios a recabar documentación administrativa. Nuevas perspectivas jurisprudenciales a raíz de su creciente judicialización

2019

For just over five years, for the first time in our democratic history, the right of parliamentarians to collect documentation from public administrations has been judicialized in the contentious-administrative way. A cluster of cases with a single common focus: tense government-parliament relations in the Valencian Community. This conflict has led, in the course of these years, to a high number of resolutions of both the Superior Court of Justice of the Valencian Community and the Supreme Court. A total of more than forty sentences that have had a considerable impact on the delimitation of this right. This article examines these judicial resolutions and offers a global analysis of the inte…

Un seminario sui mutamenti politici e istituzionali in Italia

2019

Il contributo offre le riflessioni di sintesi relative a un seminario concernente le trasformazioni politiche e istituzionali che hanno riguardato l'Italia negli ultimi anni.

Impact of different texture of polypropylene mesh on the inflammatory response.

2008

Tracing keratin evolution: catalog, expression patterns and primary structure of shark (Scyliorhinus stellaris) keratins.

1998

We have studied individual keratins of an elasmobranch, the shark Scyliorhinus stellaris (the lesser-spotted dogfish). From various shark tissues, notably skin and stomach, cytoskeletal proteins were isolated and then separated by two-dimensional polyacrylamide gel electrophoresis. Using complementary keratin blot-binding assays and immunoblotting, among these proteins we identified a variety of type I and type II keratins. According to their tissue-specific expression, we distinguished Is and IIs keratins from IE and IIE keratins ("S" and "E" from "simple epithelial" and "epidermal", respectively). Guinea pig antibodies which in immunoblots specifically labeled the entire range of identifi…

Distribution patterns of vimentin-immunoreactive structures in the human prosencephalon during the second half of gestation.

1999

Neuronal migration is guided by long radially oriented glial fibres. During late stages of development radial glial cells are transformed into astrocytes. A predominant intermediate filament protein within radial glial cells and immature astrocytes is vimentin. In this study fetal brain sections were used to demonstrate the transient features of vimentin-positive radial glia. In the lower half of the cerebral wall of the 6th gestational month bundles, curvature, and crossing of vimentin-positive fibres are regularly seen. Moreover, fibres terminating on vessels are observed. In the upper half fibres are radially oriented; when ascending towards the pial surface the number and diameter of fi…

Analysis of NO synthase expression in neuronal, astroglial and fibroblast-like derivatives differen-tiating from PCC7-Mzl embryonic carcinoma cells

1999

We studied the expression of the NO synthase isoforms in an in vitro model of neural development using RT-PCR, Western blot and immu- nohistochemistry. Murine PCC7-Mzl cells (Jostock et al., Eur. J. Cell Biol. 76, 63–76,1998) differentiate in the presence of all-trans retinoic acid and dibutyryl cAMP along the neural pathway into neuron-like, fibroblast-like and astroglia-like cells. Undifferentiated cells showed immunofluorescent staining for neuronal-type NOSI and endothelial- type NOS III. This expression pattern was retained in those cells differ entiating into neurofilament- and tau protein-positive neuronal cells. Thymocyte alloantigen (Thyl.2/CD 90.2)-positive Fibroblasts, appearing …

Vimentin and desmin of a cartilaginous fish, the shark Scyliorhinus stellaris: Sequence, expression patterns and in vitro assembly

2002

In the shark Scyliorhinus stellaris we have biochemically identified and cDNA-cloned orthologs of human vimentin and desmin, SstV and SstD, as deduced from immunoblotting and sequence alignment with teleost, frog and human vimentin and desmin, respectively. This allowed us to further clarify the relationship of previously identified lower vertebrate intermediate filament proteins to mammalian vimentin and desmin. Immunofluorescence microscopy with antibodies H5 and VIM13.2 showed vimentin expression in shark eye and brain and absence in epithelia, which resembles the situation in higher vertebrates. In addition, SstV is expressed in many mesenchymal cell types which corresponds to the case …

Dissection of keratin dynamics: different contributions of the actin and microtubule systems.

2005

It has only recently been recognized that intermediate filaments (IFs) and their assembly intermediates are highly motile cytoskeletal components with cell-type- and isotype-specific characteristics. To elucidate the cell-type-independent contribution of actin filaments and microtubules to these motile properties, fluorescent epithelial IF keratin polypeptides were introduced into non-epithelial, adrenal cortex-derived SW13 cells. Time-lapse fluorescence microscopy of stably transfected SW13 cell lines synthesizing fluorescent human keratin 8 and 18 chimeras HK8-CFP and HK18-YFP revealed extended filament networks that are entirely composed of transgene products and exhibit the same dynamic…

Zebrafish vimentin: molecular characterization, assembly properties and developmental expression

1998

To provide a basis for the investigation of the intermediate filament (IF) protein vimentin in one of the most promising experimental vertebrate systems, the zebrafish (Danio rerio), we have isolated a cDNA clone of high sequence identity to and with the characteristic features of human vimentin. Using this clone we produced recombinant zebrafish vimentin and studied its assembly behaviour. Unlike other vimentins, zebrafish vimentin formed unusually thick filaments when assembled at temperatures below 21 degrees C. At 37 degrees C few filaments were observed, which often also terminated in aggregated masses, indicating that its assembly was severely disturbed at this temperature. Between 21…

Determinación y definición de cuentas de las Cortes de 1329 en 1332: antecedentes de la Generalidad valenciana. La fiscalidad territorial y las impos…

2004

The year 1329 can be considered the starting point in the establishment of the <i>Generalitat Valenciana</i>. It is in this year when the first steps are taken toward the creation of a taxation system, starting with the regulation of local taxes.<br><br>A partir de 1329, se pueden establecer los pasos encaminados a determinar el origen de la Generalitat Valenciana, debido a que se darán las primeras pautes del procedimiento de implantación del sistema fiscal de dicho territorio a partir de la normativa de las imposiciones locales.