Search results for "Laminaribiose"

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Reaggregation and binding of cell wall proteins from Candida albicans to structural polysaccharides

1998

Urea or hot sodium dodecyl sulphate extracted a significant amount of the same proteins from the matrix of the cell wall of the yeast form and mycelial cells of Candida albicans. Gel filtration analysis of the urea-extracted proteins revealed that they occurred in the form of large complexes which were unaffected by up to 8 M urea. Among them, proteins en route to becoming covalently associated within the wall scaffold were identified by their reaction with specific antibodies. When urea was removed by dialysis, some of these proteins specifically reassociated into large aggregates which bound strongly with ConA, whereas others remained soluble in smaller associated products. The ability of…

Blotting WesternChitinPlasma protein bindingPolysaccharideBinding CompetitiveMicrobiologyFungal ProteinsCell wallchemistry.chemical_compoundChitinCell WallCandida albicansConcanavalin AUreaCandida albicansGlucansMolecular BiologyLaminaribiosePolyacrylamide gel electrophoresisAntibodies FungalGlucanchemistry.chemical_classificationbiologyMembrane ProteinsSodium Dodecyl SulfateGeneral Medicinebiology.organism_classificationMicroscopy ElectronMicroscopy FluorescenceSolubilitychemistryBiochemistryChromatography GelElectrophoresis Polyacrylamide GelProtein BindingResearch in Microbiology
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