Search results for "Layers"
showing 10 items of 335 documents
Identification of a putative membrane-inserted segment in the alpha-toxin of Staphylococcus aureus.
1994
To gain a fuller understanding of the regions of the Staphylococcus aureus alpha-toxin important in pore formation, we have used Forster dipole-dipole energy transfer to demonstrate that a central glycine-rich region of alpha-toxin (the so-called "hinge" region) inserts deeply into the bilayer on association of toxin with liposomes. Mutant alpha-toxins with unique cysteine (C) residues at positions 69 and 130 [Palmer, M., et al. (1993) J. Biol. Chem. 268, 11959) were reacted with the C-specific fluorophore acrylodan, which acted as an energy donor. The chosen acceptor was N-(7-nitrobenz-2-oxa-13- diazol-4-yl)-1,2-bis(hexadecanoyl)-sn-glycero-3-phosphoethanolamin e (NBD-PE). Measurement of t…
Membrane fluidity and the surface properties of the lipid bilayer: ESR experiment and computer simulation
2009
Penetration of the liposome membranes formed in the gel phase from DPPC (DPPC liposomes) and in the liquid-crystalline phase from egg yolk lecithin (EYL liposomes) by the TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) and 16 DOXYL (2-ethyl-2-(15-methoxy-oxopentadecyl)-4,4-dimethyl-3-oxazolidinyloxy) spin probes has been investigated. The penetration process was followed by 120 hours at 24(0)C, using the electron spin resonance (ESR) method. The investigation of the kinetics of the TEMPO probe building into the membranes of both types of liposomes revealed differences appearing 30 minutes after the start of the experiment. The number of TEMPO particles built into the EYL liposome membranes beg…
Między Starą a Nową Ojczyzną – muzyczna aktywność cymbalistów na Dolnym Śląsku w latach 50. XX wieku
2022
W artykule omówiono zagadnienie muzycznej aktywności cymbalistów, którzy zmuszeni byli do migracji z przedwojennych kresów wschodnich (Wileńszczyzny, Galicji Wschodniej) na Dolny Śląsk po II wojnie światowej. Będąc nowymi osadnikami na tzw. Ziemiach Odzyskanych, otoczeni ludnością o różnym pochodzeniu regionalnym, cymbaliści próbowali w nowych warunkach kontynuować muzyczną działalność. W tekście ukazane są różne sposoby adaptacji do nowej sytuacji wykonawczej w nowym miejscu zamieszkania: budowanie kolejnych instrumentów, granie w kapelach ludowych składających się z członków o różnym pochodzeniu regionalnym, próby indywidualnego muzykowania oparte na wykonywaniu muzyki tanecznej z regionu…
Scrutiny of the Failure of Lipid Membranes As A Function of Headgroups, Chain Length, and Lamellarity Measured by Scanning Force Microscopy
2004
AbstractA fast, quantitative, and unambiguous screening of material properties of biomembranes using scanning force microscopy in pulsed force mode on lipid membranes is presented. The spatially resolved study of breakthrough force, breakthrough distance, adhesion, stiffness, and topography of lipid membranes as determined simultaneously by digitalized pulsed force mode provides new insight into the structure-function relationship of model membranes, which are systematically analyzed by varying chain length, lipid headgroup, and lamellarity. For this purpose, a novel unbiased analysis method is presented. A strong correlation between adhesion and breakthrough events is found on lipid bilaye…
Partitioning of Pyrene-Labeled Phospho- and Sphingolipids between Ordered and Disordered Bilayer Domains
2004
AbstractHere we have studied how the length of the pyrene-labeled acyl chain (n) of a phosphatidylcholine, sphingomyelin, or galactosylceramide affects the partitioning of these lipids between 1), gel and fluid domains coexisting in bovine brain sphingomyelin (BB-SM) or BB-SM/spin-labeled phosphatidylcholine (PC) bilayers or 2), between liquid-disordered and liquid-ordered domains in BB-SM/spin-labeled PC/cholesterol bilayers. The partitioning behavior was deduced either from modeling of pyrene excimer/monomer ratio versus temperature plots, or from quenching of the pyrene monomer fluorescence by spin-labeled PC. New methods were developed to model excimer formation and pyrene lipid quenchi…
Crystal structure and magnetism of Co(HPO3)⋅H2O : A novel layered compound of Co(II)
1990
Under the terms of the Creative Commons Attribution (CC BY) license to their work.-- et al.
Stimuli-responsive hybrid materials: breathing in magnetic layered double hydroxides induced by a thermoresponsive molecule
2014
[EN] A hybrid magnetic multilayer material of micrometric size, with highly crystalline hexagonal crystals consisting of CoAl-LDH ferromagnetic layers intercalated with thermoresponsive 4-(4-anilinophenylazo)benzenesulfonate (AO5) molecules diluted (ratio 9 : 1) with a flexible sodium dodecylsulphate (SDS) surfactant has been obtained. The resulting material exhibits thermochromism attributable to the isomerization between the azo (prevalent at room temperature) and the hydrazone (favoured at higher temperatures) tautomers, leading to a thermomechanical response. In fact, these crystals exhibited thermally induced motion triggering remarkable changes in the crystal morphology and volume. In…
Influences of baseline heart rate variability on repeated sprint performance in young soccer players
2015
AIM: The aim of this study was to assess the influence of the autonomic system on the performance of repeated sprints, involving predominantly neuromuscular power, in young soccer players. METHODS: Twelve young soccer players, selected for the Sicilian Regional Football Representative, mean age 15 years, were recruited. Baseline heart rate variability (HRV) was measured and indices of power spectral analysis (LF, HF, and ratio LF/HF) were calculated to evaluate the sympathovagal balance. The athletes performed 10 maximal 6--sec cycling sprints (RS), interspersed with 30--sec of recovery, to measure the peak power output (PP) represented by the highest power value recorded during a 6--sec sp…
Transformational Leadership, Task-Involving Climate, and Their Implications in Male Junior Soccer Players: A Multilevel Approach
2019
Despite the well-known positive consequences of transformational coaches in sport, there is still little research exploring the mechanisms through which coaches&rsquo
The effects of the Nordic hamstring exercise on sprint performance and eccentric knee flexor strength: A systematic review and meta-analysis of inter…
2021
Objectives: The primary aim of this study was to investigate the effects of the Nordic hamstring exercise (NHE) on sprint performance (i.e., 5, 10 and 20 m) and explore associations between study characteristics and sprint outcomes in team sport players. Secondary aims were to (1) investigate the effects of the NHE on eccentric strength of the knee flexors (ESKF) with categorical subgroup analysis to determine differences between recreationally, well-trained individuals and young athletes, (2) determine the relation between ESKF and sprint performance in team sport players, and (3) explore the effect of study characteristics (i.e., weekly volume, time duration and body mass) on ESKF.Methods…