Search results for "Liceo"
showing 10 items of 38 documents
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis
2020
14 p.-7 fig.-2 tab.
A Shotgun Proteomics Approach Reveals a New Toxic Role for Alzheimer's Disease Aβ Peptide: Spliceosome Impairment.
2017
Proteomic changes have been described in many neurodegenerative diseases, including Alzheimer's disease (AD). However, the early events in the onset of the pathology are yet to be fully elucidated. A cell model system in which LAN5 neuroblastoma cells were incubated for a short time with a recombinant form of Aβ42 was utilized. Proteins extracted from these cells were subjected to shotgun proteomics analysis by LTQ-Orbitrap-MS followed by label-free quantitation. By bioinformatics tools we found that the most significant of those found to be up-regulated were related to cytoskeletal dynamics (Rho related) and membrane-related processes. The most significant of the down-regulated proteins we…
IS THE MACROSCOPIC CLASSIFICATION OF FLINT USEFUL? A PETROARCHAEOLOGICAL ANALYSIS AND CHARACTERIZATION OF FLINT RAW MATERIALS FROM THE IBERIAN NEOLIT…
2009
Casa Montero is a mining complex located outside Madrid (Spain), dated from the Early Neolithic (c. 5400-5000 cal bc). An area of some 4 ha has been investigated and some 4000 shafts recorded, of which 324 have been excavated. The characterization of its raw flint materials and the establishment of its diagnostic features are indispensable in the reconstruction of the distribution of the mine's products beyond the immediate site. This work reports the geological study of the mine's Miocene flint layers and their petrological characterization. Archaeological samples from the mine's shafts were classified according to macroscopic features and petrological characteristics.
New stratigraphical data on the Middle-Late Jurassic biosiliceous sediments from the Sicanian basin, Western Sicily (Italy)
2008
The reported data present the stratigraphy of several sections across a Middle–Late Jurassic Radiolaritic Unit, well exposed in different thrust sheets pertaining to the Maghrebian chain of Southwestern Sicily. The aim was to define the chronostratigraphical distribution of the Jurassic biosiliceous sedi- mentation in the Sicanian palaeogeographical zone, a deep water basin belonging to the Southern Tethys continental margin. The radiolarian biostratigraphy indicates that the switching from carbonate to siliceous sedimentation in the Sicanian Basin is referable to the Bajocian, as shown by the section of Campofiorito, near Corleone. The biostratigraphical dataset allows the correlation betw…
La escritura expresiva en la orientación. Una metodología educativa para la construcción del proyecto de vida personal y profesional
2020
La scrittura espressiva adottata a scuola come strumento di autorientamento dell’adolescente di 17-18 anni non sostituisce l’intervento educativo dell’insegnante, piuttosto può considerarsi una tecnica efficace per raccogliere tutte le informazioni necessarie con cui formare, con il consenso dell’alunno e insieme a lui, un quadro esaustivo in cui includere lo stato attuale delle sue competenze, le sue attitudini, i suoi interessi, le sue motivazioni, la sua storia formativa, il suo profilo di personalità, i suoi limiti, nonché i suoi obiettivi e le modalità concrete con cui egli pensa di raggiungerli. Essa è pertanto un metodo educativo con cui gli studenti che si approssimano a svolgere gl…
Codon usage in the siliceous sponge Geodia cydonium: highly expressed genes in the simplest multicellular animals prefer C- and G-ending codons
2001
Among a sample of 39 Geodia cydonium (Demospongiae, Porifera) genes, with an average Gh+hC content of 51.2%, extensive structural heterogeneity and considerable variations in synonymous codon usage were found. The G + C content of coding sequences and G + C content at silent codon positions (GC3S) varied from 42.4 to 59.2% and from 35.6 to 76.5%, respectively. Correspondence analysis of 39 genes revealed that putative highly expressed genes preferentially use a limited subset of codons, which were therefore defined as preferred codons in G. cydonium. A total of 22 preferred codons for 18 amino acids with synonyms in codons were identified and they all (with one exception) end with C or G. A…
Collaborative Teaching in the Italian "Liceo Matematico": A Case Study of Co-Planning and Co-Teaching
2020
This contribution is centred on a case study of collaborative teaching, carried out among Upper Secondary School Math teachers. Here we present the context, the forms of the implemented collaboration and the effects that derived from the collaborative teaching. The used investigation tool was the semi-structured interview aimed to induce teachers to gradually reflect on their self and their teaching modus operandi. From the qualitative analysis emerged that collaborative teaching integrated different ways of teaching; it has been a stimulus for a etching “revision” and the possibility to improve the didactic-educational practice. For all teachers this way to define the teaching activities w…
All-atom simulations disentangle the functional dynamics underlying gene maturation in the intron lariat spliceosome
2018
The spliceosome (SPL) is a majestic macromolecular machinery composed of five small nuclear RNAs and hundreds of proteins. SPL removes noncoding introns from precursor messenger RNAs (pre-mRNAs) and ligates coding exons, giving rise to functional mRNAs. Building on the first SPL structure solved at near–atomic-level resolution, here we elucidate the functional dynamics of the intron lariat spliceosome (ILS) complex through multi-microsecond-long molecular-dynamics simulations of ∼1,000,000 atoms models. The ILS essential dynamics unveils (i) the leading role of the Spp42 protein, which heads the gene maturation by tuning the motions of distinct SPL components, and (ii) the critical particip…
Can multiscale simulations unravel the function of metallo-enzymes to improve knowledge-based drug discovery?
2019
Metallo-enzymes are a large class of biomolecules promoting specialized chemical reactions. Quantum-classical quantum mechanics/molecular mechanics molecular dynamics, describing the metal site at quantum mechanics level, while accounting for the rest of system at molecular mechanics level, has an accessible time-scale limited by its computational cost. Hence, it must be integrated with classical molecular dynamics and enhanced sampling simulations to disentangle the functions of metallo-enzymes. In this review, we provide an overview of these computational methods and their capabilities. In particular, we will focus on some systems such as CYP19A1 a Fe-dependent enzyme involved in estroge…
SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes
2021
Abstract Splicing is catalyzed by the spliceosome, a compositionally dynamic complex assembled stepwise on pre-mRNA. We reveal links between splicing machinery components and the intrinsically disordered ciliopathy protein SANS. Pathogenic mutations in SANS/USH1G lead to Usher syndrome—the most common cause of deaf-blindness. Previously, SANS was shown to function only in the cytosol and primary cilia. Here, we have uncovered molecular links between SANS and pre-mRNA splicing catalyzed by the spliceosome in the nucleus. We show that SANS is found in Cajal bodies and nuclear speckles, where it interacts with components of spliceosomal sub-complexes such as SF3B1 and the large splicing cofact…