Search results for "Lici"

Therapeutic Potential of AntagomiR-23b for Treating Myotonic Dystrophy

2020

Myotonic dystrophy type 1 (DM1) is a chronically debilitating, rare genetic disease that originates from an expansion of a noncoding CTG repeat in the dystrophia myotonica protein kinase (DMPK) gene. The expansion becomes pathogenic when DMPK transcripts contain 50 or more repetitions due to the sequestration of the muscleblind-like (MBNL) family of proteins. Depletion of MBNLs causes alterations in splicing patterns in transcripts that contribute to clinical symptoms such as myotonia and muscle weakness and wasting. We previously found that microRNA (miR)-23b directly regulates MBNL1 in DM1 myoblasts and mice and that antisense technology (“antagomiRs”) blocking this microRNA (miRNA) boost…

Lack of evidence of mimivirus replication in human PBMCs

2018

The Acanthamoeba polyphaga mimivirus (APMV) was first isolated during a pneumonia outbreak in Bradford, England, and since its discovery many research groups devoted efforts to understand whether this virus could be associated to human diseases, in particular clinical signs and symptoms of pneumonia. In 2013, we observed cytopathic effect in amoebas (rounding and lysis) inoculated with APMV inoculated PBMCs (peripheral blood mononuclear cell) extracts, and at that point we interpreted those results as mimivirus replication in human PBMCs. Based on these results we decided to further investigate APMV replication in human PBMCs, by transmission electron microscopy (TEM) and qPCR. No viral fac…

Membrane-Associated Enteroviruses Undergo Intercellular Transmission as Pools of Sibling Viral Genomes

2019

Summary Some viruses are released from cells as pools of membrane-associated virions. By increasing the multiplicity of infection (MOI), this type of collective dispersal could favor viral cooperation, but also the emergence of cheater-like viruses such as defective interfering particles. To better understand this process, we examined the genetic diversity of membrane-associated coxsackievirus infectious units. We find that infected cells release membranous structures (including vesicles) that contain 8–21 infectious particles on average. However, in most cases (62%–93%), these structures do not promote the co-transmission of different viral genetic variants present in a cell. Furthermore, …

More than a pore: How voltage-gated calcium channels act on different levels of neuronal communication regulation.

2021

ABSTRACT Voltage-gated calcium channels (VGCCs) represent key regulators of the calcium influx through the plasma membrane of excitable cells, like neurons. Activated by the depolarization of the membrane, the opening of VGCCs induces very transient and local changes in the intracellular calcium concentration, known as calcium nanodomains, that in turn trigger calcium-dependent signaling cascades and the release of chemical neurotransmitters. Based on their central importance as concierges of excitation-secretion coupling and therefore neuronal communication, VGCCs have been studied in multiple aspects of neuronal function and malfunction. However, studies on molecular interaction partners …

2019

Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver multiple viral genomes to the same cell simultaneously, which can have important implications for viral pathogenesis, antiviral resistance, and social evolution. However, little is known about why some viruses transmit in collective infectious units, whereas others do not. We used a simple evolutionary approach to model the potential costs and benefits of transmitting in a collect…

Eating chocolate, smelling perfume or watching video advertisement: Does it make any difference on emotional states measured at home using facial exp…

2019

Type d'article : méthodologie, recherche et revue.; International audience; The recording of facial expressions allows for implicit measurement of emotional states over time. The present study investigated whether these recordings can be acquired, using computer webcams, when testing products at home. Three types of product spaces (chocolates, perfumes and video advertisements) were evaluated at home by 44 subjects using a facial expression measurement protocol. Each product space was composed of three products. The first objective examined the feasibility of such a home-based protocol. The second objective investigated whether several products in the same product space could be characteriz…

Policiers, gendarmes et signalement descriptif. Représentations, apprentissages et pratiques d'une nouvelle technique de police judiciaire, en France…

2006

À partir des années 1880, les nouveaux procédés d’identification développés par Alphonse Bertillon à la Préfecture de police de Paris représentent des moyens modernes et efficaces de police judiciaire face à une criminalité perçue comme irrésistible. Parmi ceux-ci, le signalement descriptif, ou « portrait parlé », est réputé permettre la reconnaissance infaillible d’un individu dont les traits du visage auraient été minutieusement décrits par cette technique particulière. Fait notable, sans doute inédit et sans équivalent alors, cette technique est enseignée aux divers acteurs de la police française – policiers de la Préfecture de police, de la Sûreté générale, mais aussi gendarmes et garde…

Sort and beer: Everything you wanted to know about the sorting task but did not dare to ask

2011

author cannot archive publisher's version/PDF; International audience; In industries, the sensory characteristics of products are key points to control. The method commonly used to characterize and describe products is the conventional profile. This very efficient method requires a lot of time to train assessors and to teach them how to quantify the sensory characteristics of interest. Over the last few years, other faster and less restricting methods have been developed, such as free choice profile, flash profile, projective mapping or sorting tasks. Among these methods, the sorting task has recently become quite popular in sensory evaluation because of its simplicity: it only requires ass…

Powered by assemblage : language for multiplicity

2021

Abstract Assemblage is one way to examine complexities in today’s world. In Deleuzian thinking, assemblage refers to both the act of assembling diverse elements and the arrangements of these elements for a specific purpose. Importantly, it is the interaction between elements that allows the assemblage to become more than the sum of its parts. Applying this concept to long-term research on Cold Rush – the transformation of the Arctic commons into commodities – I argue that examining the boom, bust, and buzz around the commons can be fruitfully conceptualised and studied with assemblage. This approach brings with it an ontological shift from binaries into multiplicities and multiple temporali…

The look of writing in reading. Graphetic empathy in making and perceiving graphic traces

2021

This article presents preliminary considerations and results from a research project designed to investigate the relation between (i) gestures, (ii) graphic traces and (iii) perceptions. More specifically, the project aims to test the hypothesis that graphic traces, including handwriting, can set up graphetic empathy between writers and readers of traces across long temporal and spatial distances. Insofar as a graphic trace is lawfully related to the gesture by which it came into being, the trace itself will hold information about the gesture, which may resonate with the sensorimotor system of a perceiver as if they themselves performed the gesture. If this is in fact so, it will have impor…