Search results for "Ligo"
showing 10 items of 1427 documents
First contribute to the limnological knowledge of lentic water bodies belonging to the territory of the 'Fundacion San Ignacio del Huinay' (X region …
2008
G26/24 extracellular microvesicles contain both H1° protein and RNA
2015
Extracellular vesicles (EVs) are released into the extracellular space from both tumor and normal brain cells. By releasing EVs which contain FGF2 and VEGF1-2, astrocytes and neurons, co-cultured with brain capillary endothelial cells, are for example able to induce them to form a blood-brain barrier-like monolayer. On the other hand, membrane microvesicles (MVs) shed from G26/24 oligodendroglioma cells, when added to primary cultures of rat cortical neurons, induce neuronal damage; the damaging effects include a strong reduction of neurite outgrowth, and apoptosis in about 75% of the cells3. The same amount of shed MVs induce apoptosis in about 40% of astrocytes4. These effects are probab…
Melanoma cells release extracellular vesicle which contain H1° linker histone as well as RNA-binding proteins which bind to the H1° mRNA
2015
We previously demonstrated that G26/24 oligodendroglioma cells release EVs that contain proteins, such as FasL and TRAIL, which induce apoptosis in rat cortical neurons [1] and astrocytes [2]. We also reported that cancer cells use EVs for transferring, into the environment [3], proteins such as extracellular matrix remodelling proteases [4], and H1°, a differentiation-specific histone [5]. In particular, by releasing H1°, cells could escape differentiation cues [5]. To verify the role of EVs in releasing specific proteins and mRNAs, in this study we used as a model A375 melanoma cells. METHODS EVs were purified from cell culture media as previously reported [1, 2]. T1 RNase-protection assa…
Cancer cells can affect behaviour of neighbouring cells by transferring molecules through extracellular vesicles
2017
Most cells release into the extracellular space membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells are much more active than normal cells in producing EVs. Because of this property, they are able to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in these latter at least some transformed behavior. We previously showed that EVs produced by G26/24 oligodendroglioma cells can horizontally transfer to their neighbours radioactive proteins [2]. In addition, EVs released by these cells contain pro-apoptotic proteins, such as TRAIL and Fas-Ligand, able to induce apoptosis in…
Melanoma cells release extracellular vesicles which contain RNA-binding proteins able to bind the mRNA encoding histone H1°
2015
Extracellular vesicles (EVs) are produced by most prokaryotic and eukaryotic cells; tumour cells, however, release much higher amounts of EVs, which contain cancer-specific proteins and RNAs. Molecules carried by EVs are captured by surrounding cells, which then undergo profound phenotypic modifications. G26/24 oligodendroglioma cells release, for example, EVs containing FasL and TRAIL, which induce apoptosis in rat cortical neurons and astrocytes in culture. By metabolic labelling of cells, EV-mediated horizontal transfer of radioactive proteins was clearly demonstrated. Among the proteins present in EVs produced by oligodendroglioma cells, extracellular matrix remodelling proteases, and t…
RNA as a carrier of epigenetic information
2017
Both prokaryotic and eukaryotic cells release into the extracellular matrix membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells, in particular, use EVs to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in them transformed behaviours or killing them. G26/24 oligodendroglioma cells, for example, transfer by EVs pro-apoptotic proteins, such as TRAIL and Fas-Ligand [2], extracellular matrix remodelling proteases (such as ADAMTS) [3], and even the H1.0 histone protein [4]. Another tumour cell line, with a different tissue origin (A375 melanoma cells) releases into the medi…
Extracellular vesicles released from melanoma cells contain H1° mRNA-binding proteins, one of which is (probably) MYEF2.
2015
Release of extracellular vesicles (EVs) is a process conserved from prokaryotes to eucaryotes. Although EVs are produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which contain tumour-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL and are able to inhibit neurite outgrowth, and induce apoptosis in about 75% of rat cortical neurons [1] and 40% of astrocytes [2] in culture. By labelling proteins synthesized in one cell type, we also demonstrated EV-mediated horizontal transfer of proteins among brain cells. Interestingly, G2624 release, via EVs, extr…
La tecnologia del DNA-microarray per l'identificazione di specie microbiche su superfici e nell'aerosol di ambienti confinati
2009
La tecnologia del DNA microarray è utilizzata nei nostri laboratori per lo sviluppo di piattaforme multi-diagnostiche finalizzate alla identificazione di microrganismi. Il sistema è applicabile a campioni biologici prelevati in ambienti confinati, dove funghi e batteri rappresentano una delle componenti principali. Questi microrganismi, sono in grado di svilupparsi, in stretta relazione con determinati parametri come: i valori di temperatura e umidità relativa, le caratteristiche chimico fisiche dei materiali, e delle superfici, la tipologia di aerosol di questi ambienti. L’instaurarsi di consorzi microbici complessi e stabili oltre ad accelerare il degrado superficiale dei materiali, può c…
La città di Alphabet. Architettura per prosumers.
2021
Attraverso alcuni aspetti salienti dell’ideazione, della produzione e dell’opposizione al progetto del Toronto Quayside di Sidewalk labs, l’articolo discute l’influenza delle infrastrutture digitali per l’informazione sul progetto della casa e del quartiere. L’ampliamento delle attività svolte da remoto nello spazio domestico, enfatizzato dal quotidiano della pandemia da Covid 19, costituisce per gli oligopolisti planetari degli urban data come Alphabet un utile volano pubblicitario e un acceleratore. La fusione dei nuovi vessilli della tecnoemancipazione e della sostenibilità in ipotesi sperimentali che mirano a costruirsi sia per intero che per parti realizza un nuovo orizzonte in cui lo …
Obbligo formativo del datore di lavoro e assegnazione a nuove mansioni
2017
L'articolo esamina il contenuto dell'obbligo del datore di lavoro di impartire adeguata formazione al lavoratore, in caso di assegnazione ad altre mansioni ai sensi dell'art. 2103 c.c.