Search results for "Ligo"
showing 10 items of 1427 documents
Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model
2006
Abstract Background The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new play…
Oligonucleotides-decorated-poly(N-vinyl pyrrolidone) nanogels for gene delivery
2013
Pulsed electron-beam irradiation of a semi-dilute poly(N-vinyl pyrrolidone) (PVP) aqueous solution in the presence of acrylic acid has led to a carboxyl functionalized nanogel system. Nanoparticles hydrodynamic size and surface charge density, in water and as a function of pH, were investigated by dynamic light scattering and laser doppler velocimetry, respectively. Nanogels (NGs) were proved not to be cytotoxic at the cellular level. Indeed, they rapidly bypass the cellular membrane to accumulate in specific cell portions of the cytoplasm, in the perinuclear area. The availability of pendant carboxyl groups on the crosslinked PVP NGs core prompted us to attempt their decoration with a sing…
Cholesterol-Streptolysin O Interaction: An EM Study of Wild-Type and Mutant Streptolysin O
1998
We present transmission electron microscopical data from negatively stained specimens of cholesterol following interaction with the thiol-activated bacterial toxin streptolysin O (SLO) (wild-type and a number of cysteine substitution mutants), with and without chemical modification of the cysteine residues. Two experimental systems were used, one with an aqueous suspension of cholesterol microcrystals and the other with immobilized thin planar cholesterol crystals attached to a carbon film. In both systems the wild-type SLO and two cytolytically active mutants, Cys 530 --Ala (C530A) and Ser 101 --Cys (S101C), readily generated the characteristic SLO arc- and ring-like oligomers on the surfa…
Optical and electrical properties of oriented thin films of oligomer containing betaine-type moiety in side chain
2005
Non-linear optical and electrical properties of polymer films obtained by dipole orientation of active units are reported. Novel polar oligomer with N-(indan-1,3-dion-2-yl)pyridinium betaine (IPB) as a side group is studied. Orientation of polar groups in oligomer thin films causes an increase of the photo-induced change of surface potential on irradiation in the region of photo-induced electron transfer (PIET) where the IPB group exhibits a reversible change of the value and sign of the dipole moment. At longer wavelengths, the value of the surface potential of the oligomer may be determined by transport of photogenerated charge carriers.
Search for anisotropic gravitational-wave backgrounds using data from Advanced LIGO and Advanced Virgo's first three observing runs
2021
We report results from searches for anisotropic stochastic gravitational-wave backgrounds using data from the first three observing runs of the Advanced LIGO and Advanced Virgo detectors. For the first time, we include Virgo data in our analysis and run our search with a new efficient pipeline called {\tt PyStoch} on data folded over one sidereal day. We use gravitational-wave radiometry (broadband and narrow band) to produce sky maps of stochastic gravitational-wave backgrounds and to search for gravitational waves from point sources. A spherical harmonic decomposition method is employed to look for gravitational-wave emission from spatially-extended sources. Neither technique found eviden…
The grapevine LysM receptor-like kinase VvLYK5-1 mediates chitin-triggered immunity
2021
The establishment of defense reactions to protect plants against invading pathogens first requiresthe recognition of Microbe-Associated Molecular Patterns (MAMPs), detected by plasmamembrane-bound Pattern Recognition Receptors (PRRs). These MAMPs, also termed elicitors, areused in several biocontrol products that are gradually developing to reduce the use of chemicalpesticides in agriculture. Chitin, the main component of fungal cell walls, as well as its deacetylatedderivative, chitosan, are two chitooligosaccharides (COS) that can be found in some of theseproducts. Unfortunately, the mechanism allowing the perception of these molecules is still poorlyunderstood in Vitis vinifera, sometime…
Phosphorylation of the Goodpasture antigen by type A protein kinases.
1995
Collagen IV is the major component of basement membranes. The human alpha 3 chain of collagen IV contains an antigenic domain called the Goodpasture antigen that is the target for the circulating immunopathogenic antibodies present in patients with Goodpasture syndrome. Characteristically, the gene region encoding the Goodpasture antigen generates multiple alternative products that retain the antigen amino-terminal region with a five-residue motif (KRGDS). The serine therein appears to be the major in vitro cAMP-dependent protein kinase phosphorylation site in the isolated antigen and can be phosphorylated in vitro by two protein kinases of approximately 50 and 41 kDa associated with human …
Mipomersen: a lipid-lowering agent with a novel mechanism of action
2013
“...mipomersen is a ... valuable alternative to apheresis for patients with heterozygous familial hypercholesterolemia.”
Structural and functional diversity of Bacillus thuringiensis toxins on animal, plant and microbial cells
2018
Debido a los efectos nocivos de los insecticidas químicos en los ecosistemas y sobre los organismos beneficiosos, la implementación de agentes de control biológico ha recibido considerable atención. Entre los agentes de control biológico, se ha dedicado gran interés a los entomopatógenos, debido a su potencial para controlar plagas agrícolas y domésticas, vectores de enfermedades humanas y animales, sin introducir material no degradable en el medio ambiente. Bacillus thuringiensis (Bt) es el agente de control microbiano comercial más utilizado en la actualidad, con efectividad contra diferentes especies de insectos plaga. Las cepas de Bt producen cristales paraesporales proteináceos durante…
Ionic Liquids as Homogeneous Catalysts for Glycerol Oligomerization
2022
Ionic liquids (ILs) were used for the first time as catalysts for the glycerin condensation reaction. A series of imidazolium and ammonium ionic liquids differing in the length of the alkyl substituent (C2, C12, and C14) and the type of anion (Br−, CH3COO−, and NaHPO4−) were synthesized using a typical two-step method. The structure of the obtained ILs was confirmed by nuclear magnetic resonance 13C NMR, and their base power was determined on the basis of the Hammett function. The oligomerization of glycerin with the participation of the obtained ionic liquids and, for comparison, in the presence of a homogeneous basic catalyst Na2CO3, was carried out for 3 h at 180 °C, under a pressure of …