Search results for "Ligo"

showing 10 items of 1427 documents

Terpioside B, a difucosyl GSL from the marine sponge Terpios sp. is a potent inhibitor of NO release.

2010

Terpioside B (2a), a unique glycolipid containing two fucose residues in the furanose form in its pentasaccharide chain, was isolated from the marine sponge Terpios sp. Its complete stereostructure was solved by interpretation of mass spectrometric and NMR data along with CD and GG-MS analyses of its degradation products. Terpioside B is a potent inhibitor against LPS-induced NO release, and is considerably more active than simpler glycosphingolipids such as terpioside A and monoglucosylceramide.

LipopolysaccharidesTerpiosStereochemistryClinical BiochemistryMolecular ConformationPharmaceutical ScienceNitric Oxide01 natural sciencesBiochemistryFucoseCell Line03 medical and health scienceschemistry.chemical_compoundGlycolipidDrug DiscoveryAnimalsMolecular Biology030304 developmental biologychemistry.chemical_classification0303 health sciencesbiology010405 organic chemistryChemistryMacrophagesOrganic ChemistryAbsolute configurationGlycosphingolipidOligosaccharideMacrophage Activationbiology.organism_classificationFuranose0104 chemical sciencesPoriferaSpongeBiochemistryMolecular MedicineGlycolipidsBioorganicmedicinal chemistry
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Core oligosaccharide of Escherichia coli B—the structure required for bacteriophage T4 recognition

2015

Abstract The structure of Escherichia coli B strain PCM 1935 core oligosaccharide has been investigated by 1H and 13C NMR spectroscopy, MALDI-TOF MS and ESI MSn. It was concluded that the core oligosaccharide is a pentasaccharide with the following structure: ESI MS/MS analysis revealed that the glycine (a minor component) is linked to the →3,7)- l -α- d -Hepp-(1→ residue.

Lipopolysaccharidesanimal structuresStereochemistryElectrospray ionizationMolecular Sequence Datamedicine.disease_causeBiochemistryAnalytical ChemistryBacteriophageResidue (chemistry)13c nmr spectroscopyEscherichia colimedicineBacteriophage T4Escherichia coliChromatographybiologyStrain (chemistry)ChemistryCore oligosaccharideOrganic ChemistryGeneral Medicinebiology.organism_classificationEscherichia coli B; Core oligosaccharide; glycine; NMR; MALDI-TOF; ESI MSCarbohydrate SequenceGlycineCarbohydrate Research
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Dominant negative MORT1/FADD rescues mice from CD95 and TNF-induced liver failure

2002

Derangement of the apoptotic program is considered an important cause of liver disease. It became clear that receptor-mediated apoptosis is of specific interest in this context, and CD95 and CD120a, both members of the tumor necrosis factor (TNF) receptor superfamily, are the most prominent cell death receptors involved. The death signal is induced upon ligand binding by recruitment of caspases via the adapter molecule MORT1/FADD to the receptor and their subsequent activation. To investigate the role of MORT1/FADD in hepatocyte apoptosis, we generated transgenic mice expressing liver-specific dominant negative mutant. Mice looked grossly normal; breeding and liver development were not diff…

Lipopolysaccharidesmedicine.medical_specialtyProgrammed cell deathFas-Associated Death Domain ProteinOligonucleotidesMice TransgenicAntibodiesReceptors Tumor Necrosis FactorMiceLiver diseaseAntigens CDAlbuminsInternal medicinemedicineAnimalsfas ReceptorFADDPromoter Regions GeneticAdaptor Proteins Signal TransducingLiver injuryHepatitisMice Inbred BALB CHepatologybiologyTumor Necrosis Factor-alphamedicine.diseaseFas receptorMice Inbred C57BLEndocrinologyReceptors Tumor Necrosis Factor Type IApoptosisCaspasesbiology.proteinTumor necrosis factor alphaCarrier ProteinsLiver FailureHepatology
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Production of interleukin-6, tumor necrosis factor α and interleukin-10 in vitro correlates with the clinical immune defect in chronic hemodialysis p…

1995

Production of interleukin-6, tumor necrosis factor α and interleukin-10 in vitro correlates with the clinical immune defect in chronic hemodialysis patients. In patients with chronic renal failure alterations in monokine production are a common feature. Their clinical relevance has not yet been proven. We show here a correlation between an overproduction of interleukin-(IL)-6 and tumor necrosis factor alpha (TNFα) upon stimulation with LPS by mononuclear cells in vitro and the clinical grade of immunodeficiency found in these patients. Higher levels of IL-6 and TNFα were correlated with an immunocompromized state, that is, non-responsiveness to hepatitis B vaccination, whereas patients with…

Lipopolysaccharidesmedicine.medical_specialtyTime Factorsmedicine.medical_treatmentMolecular Sequence DataImmune systemRenal DialysisInternal medicineImmunopathologymedicineHumansInterleukin 6ImmunodeficiencyBase SequencebiologyInterleukin-6Tumor Necrosis Factor-alphabusiness.industryMonokinesVaccinationAntibodies MonoclonalHepatitis Bmedicine.diseaseRecombinant ProteinsInterleukin-10MonokineInterleukin 10CytokineEndocrinologyImmune System DiseasesNephrologyImmunologybiology.proteinTumor necrosis factor alphaOligonucleotide ProbesbusinessKidney International
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TGF-β2 silencing to target biliary-derived liver diseases

2020

ObjectiveTGF-β2 (TGF-β, transforming growth factor beta), the less-investigated sibling of TGF-β1, is deregulated in rodent and human liver diseases. Former data from bile duct ligated and MDR2 knockout (KO) mouse models for human cholestatic liver disease suggested an involvement of TGF-β2 in biliary-derived liver diseases.DesignAs we also found upregulated TGFB2 in liver tissue of patients with primary sclerosing cholangitis (PSC) and primary biliary cholangitis (PBC), we now fathomed the positive prospects of targeting TGF-β2 in early stage biliary liver disease using the MDR2-KO mice. Specifically, the influence of TgfB2 silencing on the fibrotic and inflammatory niche was analysed on m…

Liver CirrhosisATP Binding Cassette Transporter Subfamily B2312Cholangitis SclerosingPrimary sclerosing cholangitisMiceTransforming Growth Factor beta2Liver diseasePrimary biliary cirrhosisCholestasisFibrosisDrug DiscoveryTGF beta signaling pathwayHepatic Stellate CellsAnimalsHumansMedicineGene silencingGene Silencing1506TGF-betaddc:610Mice KnockoutHepatologybiologyLiver Cirrhosis Biliarybusiness.industryfibrosisGastroenterologyprimary sclerosing cholangitisTransforming growth factor betaOligonucleotides Antisensemedicine.diseaseUp-Regulationprimary biliary cirrhosisDisease Models AnimalGene Expression RegulationCancer researchbiology.proteincholestasisbusinessGut
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Triple therapy in treatment-experienced patients with HCV-cirrhosis in a multicentre cohort of the French Early Access Programme (ANRS CO20-CUPIC) – …

2013

International audience; Background & AimsIn phase III trials, the safety profile of triple therapy (pegylated interferon/ribavirin with boceprevir or telaprevir) seems to be similar in HCV treatment-experienced cirrhotic and non-cirrhotic patients, but few cirrhotics were included. We report the week 16 safety and efficacy analysis in a cohort of compensated cirrhotics treated in the French Early Access Programme.Methods674 genotype 1 patients, prospectively included, received 48 weeks of triple therapy. The analysis is restricted to 497 patients reaching week 16.ResultsA high incidence of serious adverse events (40.0%), and of death and severe complications (severe infection or hepatic dec…

Liver CirrhosisMaleCirrhosisBlood transfusionmedicine.medical_treatment[SDV]Life Sciences [q-bio]Chronic hepatitis CGastroenterologyTelaprevirTelaprevirCohort Studieschemistry.chemical_compound0302 clinical medicinePegylated interferonMedicineProspective StudiesAged 80 and overBoceprevirMiddle AgedViral Load3. Good healthTreatment OutcomeCirrhosis030220 oncology & carcinogenesisCohort030211 gastroenterology & hepatologyDrug Therapy CombinationFemaleFranceSafetyOligopeptidesmedicine.drugAdultmedicine.medical_specialtySerine Proteinase InhibitorsProlineAntiviral Agents03 medical and health sciencesInternal medicineBoceprevirRibavirinHumansAdverse effectAgedHepatologybusiness.industryRibavirinInterferon-alphaHepatitis C Chronicmedicine.diseaseSurgeryTreatmentchemistrybusiness
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Effectiveness of telaprevir or boceprevir in treatment-experienced patients with HCV genotype 1 infection and cirrhosis. : Triple therapy in HCV geno…

2014

International audience; BACKGROUND & AIMS: We investigated the effectiveness of the protease inhibitors peginterferon and ribavirin in treatment-experienced patients with hepatitis C virus (HCV) genotype 1 infection and cirrhosis. METHODS: In the Compassionate Use of Protease Inhibitors in Viral C Cirrhosis study, 511 patients with HCV genotype 1 infection and compensated cirrhosis who did not respond to a prior course of peginterferon and ribavirin (44.3% relapsers or patients with viral breakthrough, 44.8% partial responders, and 8.0% null responders) were given either telaprevir (n = 299) or boceprevir (n = 212) for 48 weeks. We assessed percentages of patients with sustained viral respo…

Liver CirrhosisMaleCirrhosisComorbidityHepacivirusmedicine.disease_causeGastroenterologyPolyethylene GlycolsTelaprevirCohort Studieschemistry.chemical_compound0302 clinical medicinePegylated interferonboceprevirProspective StudiesTreatment FailureAged 80 and overGastroenterologyMiddle AgedRecombinant Proteins3. Good healthTreatment Outcome030220 oncology & carcinogenesisDrug Therapy CombinationFemale030211 gastroenterology & hepatologyOligopeptidesmedicine.drugAdultmedicine.medical_specialtyGenotypeProlineHepatitis C virusAntiviral Agents03 medical and health sciencesBoceprevirInternal medicineRibavirinmedicinechronic hepatitis CHumanstelaprevirAdverse effect[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyAgedHepatologybusiness.industrycirrhosisRibavirinInterferon-alphaOdds ratioHepatitis C Chronicmedicine.diseasechemistryMultivariate AnalysisImmunologybusinessFollow-Up StudiesGastroenterology
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Hepatocyte-Specific Smad7 Expression Attenuates TGF-β–Mediated Fibrogenesis and Protects Against Liver Damage

2008

Background & Aims The profibrogenic role of transforming growth factor (TGF)-β in liver has mostly been attributed to hepatic stellate cell activation and excess matrix synthesis. Hepatocytes are believed to contribute to increased rates of apoptosis. Methods Primary hepatocyte outgrowths and AML12 cells were used as an in vitro model to detect TGF-β effects on the cellular phenotype and expression profile. Furthermore, a transgenic mouse model was used to determine the outcome of hepatocyte-specific Smad7 expression on fibrogenesis following CCl 4 -dependent damage. Samples from patients with chronic liver diseases were assessed for (partial) epithelial-to-mesenchymal transition (EMT) in h…

Liver CirrhosisMaleTime FactorsCell SurvivalApoptosisMice TransgenicBiologyCell LineSmad7 ProteinMiceTransforming Growth Factor betaFibrosismedicineAnimalsHumansSchistosomiasisEpithelial–mesenchymal transitionCarbon TetrachlorideCells CulturedOligonucleotide Array Sequence AnalysisR-SMADHepatologyGene Expression ProfilingGastroenterologyHepatitis Bmedicine.diseaseHepatic stellate cell activationMice Inbred C57BLCTGFDisease Models AnimalPhenotypemedicine.anatomical_structureHepatocyteCell TransdifferentiationHepatocytesCancer researchHepatic stellate cellCollagenTransforming growth factorGastroenterology
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High efficacy and safety of triple therapy in HCV genotype 1 and moderate fibrosis: a multicenter study of clinical practice in Spain.

2015

Background and rational. Telaprevir-based therapy (TBT) has been extensively evaluated in clinical trials. So we designed a study to compare the efficacy and safety of TBT between patients with moderate fibrosis and those suffering from advanced fibrosis in clinical practice. A multicenter observational and ambispective study was conducted. It included 582 patients with chronic hepatitis C genotype 1, 214 with fibrosis F2, and 368 with F3/F4 (F3: 148; F4: 220). Results. The mean patient age was 55 years, 67% male. Type of prior response was 22% naive, 57% relapsers, and 21% partial/null responders, 69% had high viral load (> 800,000 IU/mL). HCV genotypes were 1a (19%), 1b (69%), and 1 (12%)…

Liver CirrhosisMaleTime FactorsSpecialties of internal medicineHepacivirusGastroenterologySeverity of Illness IndexTelaprevirFibrosisRisk FactorsGenotypeGeneral MedicineMiddle AgedViral LoadTreatment OutcomeRC581-951Hepatitis C genotype 1RNA ViralDrug Therapy CombinationFemaleTelaprevir triple therapyModerate fibrosisViral loadOligopeptidesmedicine.drugAdultmedicine.medical_specialtyGenotypeAntiviral AgentsSafety and efficacyYoung AdultInternal medicinemedicineHumansAdverse effectAgedHepatologybusiness.industryInterleukinsHepatitis C Chronicmedicine.diseaseSurgeryDiscontinuationClinical trialSpainObservational studyInterferonsbusinessBiomarkersAnnals of hepatology
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Haemophagocytic syndrome in a liver transplant patient during treatment with Telaprevir.

2013

Haemophagocytic syndrome (HS) is a rare disease that is often fatal despite treatment. HS is characterized by fevers, lymphadenopathy, hepatosplenomegaly, cytopenias and hyperferritinaemia due to deregulated activation and proliferation of macrophages, leading to uncontrolled phagocytosis of platelets, erythrocytes, lymphocytes, and their hematopoietic precursors throughout the reticuloendothelial system. Mycobacterium tuberculosis-associated HS is a rare and underdiagnosed association with only 39 cases reported. We describe a case of HS associated with disseminated Mycobacterium tuberculosis in the setting of post-liver transplantation anti-hepatitis C therapy with pegylated interferon (p…

Liver CirrhosisMaleTuberculosisTime Factorsmedicine.medical_treatmentHepatosplenomegalyAntitubercular AgentsSpecialties of internal medicineHepacivirusLiver transplantationVHCAntiviral AgentsLymphohistiocytosis HemophagocyticTelaprevirTelaprevirchemistry.chemical_compoundFatal OutcomePegylated interferonRisk FactorsmedicineHumansTuberculosisHepatologyHaemophagocytic syndromebusiness.industryRibavirinGeneral MedicineMycobacterium tuberculosisMiddle Agedmedicine.diseaseHepatitis CLiver TransplantationTransplantationchemistryRC581-951ImmunologyDrug Therapy CombinationVirus Activationmedicine.symptombusinessOligopeptidesImmunosuppressive Agentsmedicine.drugRare diseaseAnnals of hepatology
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