Search results for "Lope"
showing 10 items of 2014 documents
Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.
2008
To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
2021
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …
Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1.
1995
Syncytial mutations of herpes simplex virus type 1 (HSV-1) strains ANG, ANG path, HFEM, tsB5 and HSZP cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein B (gB), within the syn 3 locus. These strains are so far the only ones which show the phenotype ‘fusion from without’ (FFWO): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. In this report we detected, using the recombinants 27/III and K-7, that an amino acid exchange from Ala to Val at aa position 854 of gB is the main determinant for FFWO activity of strains ANG, ANG path and recombinant K-7. The transfer of this muta…
SARS-CoV-2 envelope protein topology in eukaryotic membranes
2020
Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
We Shape Our Tools, and Thereafter Our Tools Shape Us
2017
The current and incoming editors in chief present the articles of this current issue of Human Technology. Additionally, they discuss the human component of recent technological macrotrends and the journal’s editorial interests in responding to and exploring them. During Editor in Chief Pertti Hurme’s 3-year term, research reflecting a wide range of topics has been published in Human Technology. This online journal, proudly open-access since its beginning, and will continue to invite innovative and interesting manuscripts for open-access publishing under its new editor in chief, Jukka Jouhki.
Prescription opioid use and employment : A nationwide Finnish register study
2021
Background: The secular decline in labor market participation and the concurrent increase in opioid use in many developed countries have sparked a policy debate on the possible connection between these two trends. We examined whether the use of prescription opioids was connected to labor market outcomes relating to participation, employment and unemployment among the Finnish population. Methods: The working-age population (aged 19–64 years) living in Finland during the period 1995–2016 was used in the analyses (consisting of 67 903 701 person-year observations). Lagged values of prescription opioid use per capita were used as the exposure. Instrumental variables (IV) estimation method was u…
Social integration and interpropositional connection
1997
International audience; The idea put forward in this exploratory research is that connectives, far from appearing haphazardly as the subject changes ideas, appear just like other operators in the discursive styles (Bronckart, 1985) linked with the activation of programs that might be called cognitive discursive (Ghiglione, 1988).
Umowa deweloperska – początek biegu terminu na stwierdzenie wad lokalu mieszkalnego, domu jednorodzinnego i gruntu
2022
W artykule rozważona jest kwestia jaki moment/momenty należałoby uznać za początek biegu terminu na stwierdzenie wad dwóch przedmiotów umowy deweloperskiej, tj. lokalu mieszkalnego/domu jednorodzinnego (1) oraz gruntu (2), na którym dom jednorodzinny/budynek, w którym znajduje się lokal mieszkalny, jest posadowiony. Wyżej wskazane zagadnienie spotkało się już z komentarzem doktryny, ale jedynie w odniesieniu do budynku . Tym samym, niniejsze opracowanie stanowi uzupełnienie wspomnianych rozważań