Search results for "MAI"
showing 10 items of 6279 documents
Expression of homeobox-containing genes in the sea urchin (Parancentrotus lividus) embryo
1994
Two homeobox-containing genes that belong to different homeodomain classes have been isolated from a sea urchin geonomic library. One, PlHbox11, is the sea urchin homologue of the human and mouse Hox B3 gene, the other, PlHbox12, shows about 55% identity with paired class genes. Expression profile analysis of the two sea urchin Hbox genes suggests that they play different roles during embryogenesis. In fact, PlHbox11 transcripts are rare and are detected only in the pluteus larva and in the Aristotle's lantern and intestine of the adult. The PlHbox12 gene is, on the contrary, transiently expressed in the very early embryo already at the four cell stage; it accumulates at the 64 cell stage a…
Does Glycemic Control Modulate the Impairment of NLRP3 Inflammasome Activation in Type 2 Diabetes?
2019
Since mitochondrial dysfunction is associated with NOD-like receptor family protein 3 (NLRP3) activation in type 2 diabetes (T2D), which can eventually lead to an impaired immune response, we set out to determine if glycemic control modulates the effects of T2D on the NLRP3 inflammasome. We have studied leukocytes from 61 diabetic patients [25 with glycated hemoglobin (HbA(1c)) 7% and 36 with HbA(1c) 8%] and 40 healthy controls. Total and mitochondrial reactive oxygen species (ROS) production was enhanced in T2D patients, and mitochondrial ROS was more pronounced in those with poor glycemic control. Levels of gene and protein expression of NLRP3 were decreased in both diabetic groups and mo…
Comparative lipidomics and proteomics analysis of platelet lipid rafts using different detergents
2016
Lipid rafts play a pivotal role in physiological functions of platelets. Their isolation using nonionic mild detergents is considered as the gold standard method, but there is no consensual detergent for lipid raft studies. We aimed to investigate which detergent is the most suitable for lipid raft isolation from platelet membrane, based on lipidomics and proteomics analysis. Platelets were obtained from healthy donors. Twelve sucrose fractions were extracted by three different detergents, namely Brij 35, Lubrol WX, and Triton X100, at 0.05% and 1%. After lipidomics analysis and determination of fractions enriched in cholesterol (Ch) and sphingomyelin (SM), proteomics analysis was performed…
Impact of ticagrelor on P2Y1 and P2Y12 localization and on cholesterol levels in platelet plasma membrane
2017
Ticagrelor is an antiplatelet agent that inhibits platelet activation via P2Y12 antagonism. There are several studies showing that P2Y12 needs lipid rafts to be activated, but there are few data about how ticagrelor impacts lipid raft organization. Therefore, we aimed to investigate how ticagrelor could impact the distribution of cholesterol and consequently alter the organization of lipid rafts on platelet plasma membranes. We identified cholesterol-enriched raft fractions in platelet membranes by quantification of their cholesterol levels. Modifications in cholesterol and protein profiles (Flotillin 1, Flotillin 2, CD36, P2Y1, and P2Y12) were studied in platelets stimulated by ADP, treate…
Large platelets but not putative endothelial progenitor cells are associated with low strut coverage after drug-eluting stent implantation
2015
Objectives This study assessed whether different subsets of circulating endothelial and putative endothelial progenitor cells (CEC and EPC) correlate with stent strut coverage (SSC) using second generation optical coherence tomography (OCT). Background Due to the lack of imaging modalities with a resolution down to the magnitude of a few cells, the influence of EPC on endothelialisation of drug-eluting stents has not been assessed in patients. Methods In 37 patients, SSC of everolimus-eluting stents was assessed by OCT 5-7months after stent implantation. Different subsets of EPC (CD34(+)KDR(+), CD34(+)KDR(+)CD45(dim), CD133(+), CD3(+)CD31(+)), CEC (CD31(+)CD45(-)CD146(+)), and CD31(+)CD45(-…
Deciphering of ADP-induced, phosphotyrosine-dependent signaling networks in human platelets by Src-homology 2 region (SH2)-profiling.
2012
Tyrosine phosphorylation plays a central role in signal transduction controlling many important biological processes. In platelets, the activity of several signaling proteins is controlled by tyrosine phosphorylation ensuring proper platelet activation and aggregation essential for regulation of the delicate balance between bleeding and hemostasis. Here, we applied Src-homology 2 region (SH2)-profiling for deciphering of the phosphotyrosine state of human platelets activated by adenosine diphosphate (ADP). Applying a panel of 31 SH2-domains, rapid and complex regulation of the phosphotyrosine state of platelets was observed after ADP stimulation. Specific inhibition of platelet P2Y receptor…
F-centru un irīdija piemaisījumu aprēķini no pirmajiem principiem gallija oksīdā polimorfos
2022
Pēdējā laikā gallijā oksīds (Ga2O3) kļuva par vienu no visaktīvāk pētāmiem materiāliem. Iemesls ir tā konkurētspējīgās elektroniskās īpašības kā, piemēram, plata aizliegtā zona, augsts caursites spriegums, viegla lādiņnesēju koncentrāciju kontrole un augsta termiskā stabilitāte. Šo īpašību dēļ, Ga2O3 ir potenciāls kandidāts uz pielietojumiem augstas jaudas elektroniskās ierīcēs. Parasti Ga2O3 kristālus audzē izmantojot Čohralska metodi, kurā tiek izmantots irīdija (Ir) tīģelis. Šī iemesla dēļ Ir ir bieži klāt Ga2O3 kristālos kā netīšs dopants. Šajā darbā ir pētītā Ir aizvietošanas defektu, ka arī F-centru ietekme uz Ga2O3 elektronisku struktūru dažādās kristāliskās fāzēs, izmantojot blīvuma…
Facility management, outsourcing and contracting overview
2009
The discipline of facility management has basically originated from the experience of the field and most of the business models are driven from the American market. The majority of surveys agree that organizational objectives vary according to different business environments. The link between a given company and its facility management department has been studied in details with a widespread agreement on the importance of tailoring to the specific context the facility management organizational model and the approach to the relationships with suppliers. In Europe two Technical Committees of the Comite Europeen de Normalisation (CEN) are currently adding to the body of knowledge terminologies…
Multidomain BEM for crack analysis in stiffened anisotropic plates.
2014
The present paper is concerned with the application of a boundary element model for the analysis of cracks in stiffened composite panels. The panel stiffeners are reduced to equivalent strips and the multidomain technique is used to model panel zones presenting different properties (skin and stiffeners equivalent strip). Also the crack is modeled exploiting the multidomain formulation. Evaluation of stress intensity factors is performed for representative problems.
La resale price maintenance nel commercio librario. Limitare la concorrenza per promuovere la cultura?
2013
Traendo spunto dalle vicende della legge n. 128/2011, che limita lo sconto massimo praticabile al pubblico sul prezzo di copertina dei libri, pprovata con largo consenso e presto abrogata nell’indifferenza generale, l’articolo propone una approfondita analisi del modello nel quale tale legge si inscrive e delle soluzioni alternative che si riscontrano nel contesto europeo. L’indagine intende, quindi, riflettere sulla fondatezza degli assunti tradizionalmente posti alla base della difesa della Rpm libraria. Taking the cue from the events of the Law n. 128/2011, limiting the discounts on books cover price, which was passed by broad consensus and soon implicitly repealed amid the general indif…