Search results for "MICE"

showing 10 items of 6027 documents

Genome-wide association study and mouse expression data identify a highly conserved 32 kb intergenic region between WNT3 and WNT9b as possible suscep…

2014

Item does not contain fulltext Bladder exstrophy-epispadias complex (BEEC), the severe end of the urorectal malformation spectrum, has a profound impact on continence as well as sexual and renal functions. It is widely accepted that for the majority of cases the genetic basis appears to be multifactorial. Here, we report the first study which utilizes genome-wide association methods to analyze a cohort comprising patients presenting the most common BEEC form, classic bladder exstrophy (CBE), to identify common variation associated with risk for isolated CBE. We employed discovery and follow-up samples comprising 218 cases/865 controls and 78 trios in total, all of European descent. Our disc…

genetics [Wnt3 Protein]Genome-wide association studyGenomeConserved sequenceMiceIntergenic regionMedizinische FakultätSALL1 protein humanGenetics (clinical)Conserved SequenceGeneticsgenetics [Wnt Proteins]Association Studies ArticlesGeneral Medicinegenetics [Transcription Factors]genetics [European Continental Ancestry Group]WNT3 protein humanReconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]metabolism [Wnt3 Protein]Medical geneticsmedicine.medical_specialtygenetics [White People]WNT9B protein human-BiologyPolymorphism Single NucleotideWhite Peopleembryology [Genitalia]Wnt3 Proteinpathology [Bladder Exstrophy]metabolism [Wnt Proteins]genetics [Bladder Exstrophy]ddc:570GeneticsmedicineAnimalsHumansGenetic Predisposition to Diseaseddc:610GenitaliaMolecular BiologyGeneBase SequenceBladder ExstrophyCase-control studymedicine.diseasemetabolism [Genitalia]Bladder exstrophyWnt ProteinsReconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10]Case-Control StudiesGenome-Wide Association StudyTranscription Factors
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Phase petrology reveals shallow magma storage prior to large explosive silicic eruptions at Hekla volcano, Iceland

2017

Abstract Understanding the conditions that culminate in explosive eruptions of silicic magma is of great importance for volcanic hazard assessment and crisis mitigation. However, geological records of active volcanoes typically show a wide range of eruptive behavior and magnitude, which can vary dramatically for individual eruptive centers. In order to evaluate possible future scenarios of eruption precursors, magmatic system variables for different eruption types need to be constrained. Here we use petrological experiments and microanalysis of crystals to clarify the P – T – x state under which rhyodacitic melts accumulated prior to the H3 eruption; the largest Holocene Plinian eruption of…

geographyVulcanian eruptionExplosive eruptiongeography.geographical_feature_category010504 meteorology & atmospheric sciencesGeochemistrySilicicMagma chamber010502 geochemistry & geophysics01 natural sciencesGeophysicsVolcanoSpace and Planetary ScienceGeochemistry and PetrologyPumiceMagmaddc:550Earth and Planetary Sciences (miscellaneous)PhenocrystPetrologyGeology0105 earth and related environmental sciencesEarth and Planetary Science Letters
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Predation risk and habitat selection of Australian house mice , Mus domesticus , during an incipient plague: desperate behaviour due to food depletion

2002

We studied habitat selection and foraging behaviour of the house mouse (Mus domesticus) related to increasing mouse densities and depleting food resources over the breeding season. The study was conducted during the increase phase of an incipient outbreak of mice in a grain-growing area of southeastern Australia. A 3-year rotation created a mosaic of large paddocks of grain crop, pasture, and fallow. The narrow fence lines between paddocks provide an important stable habitat for the mice. We monitored population densities with live-trapping and habitat preference by measuring giving-up densities (GUD) using artificial food patches. Food patches were established in crop fields, fence lines, …

geographygeography.geographical_feature_categoryEcologyForagingBiologyPasturePopulation densityOptimal foraging theoryPredationAgronomyHabitatSeasonal breederHouse miceEcology Evolution Behavior and SystematicsOikos
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The holocene marine record of unrest, volcanism, and hydrothermal activity of campi flegrei and somma-vesuvius

2020

Abstract This chapter illustrates the marine record of a spectrum of volcanic, hydrothermal, and sedimentary features that characterize the Latest Pleistocene–Holocene evolution of the Naples Bay offshore Campi Flegrei and Somma–Vesuvius. The work is based on review of previous literature integrated with interpretation of new high-resolution marine Digital Terrain Models (DTMs) derived from swath bathymetry surveys and high-resolution reflection seismic profiles calibrated with marine gravity core data. Seismic profiles from Pozzuoli Bay provide detailed images of the ring fault system and resurgent dome associated with the evolution of the Neapolitan Yellow Tuff (NYT) collapse caldera and …

geographygeography.geographical_feature_categorySettore GEO/02 - Geologia Stratigrafica E SedimentologicaCampania volcanismResurgent domeSettore GEO/03 - Geologia StrutturaleGeochemistrySommavesuviusPyroclastic rockEastern tyrrhenian marginVolcanismFault (geology)Naples baySeafloor spreadingVolcanoPumiceCampi flegreiHolocene marine recordCalderaGeology
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Using exomarkers to assess mitochondrial reactive species in vivo

2014

Background:\ud The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging.\ud \ud Scope of review:\ud One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and a…

green fluorescent proteinMitochondrionMitoPmedicine.disease_causeBiochemistryTPMPMicemethyltriphenylphosphoniumMitoBchemistry.chemical_classification02 Physical SciencesbiologyROSsuperoxide dismutaseMitochondriaelectron paramagnetic resonanceBiochemistryBiological MarkersMolecular probe3-(dihydroxyboronyl)benzyltriphenylphosphonium bromideBiochemistry & Molecular BiologyBiophysicsGFPModels BiologicalTPPSuperoxide dismutaseIn vivoOxidative damagemedicineAnimalsSOD4-HNEMolecular BiologyExomarkerReactive oxygen species(3-hydroxybenzyl)triphenylphosphonium bromideMass spectrometry0601 Biochemistry And Cell Biology06 Biological Sciences4-hydroxynonenalIn vitroOxidative StresschemistryMolecular Probesbiology.proteinEPRtriphenylphosphonium cationReactive oxygen speciesEx vivoOxidative stressBiomarkersBiochimica et Biophysica Acta (BBA) - General Subjects
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Prolonging in utero-like oxygenation after birth diminishes oxidative stress in the lung and brain of mice pups☆

2013

Background Fetal-to-neonatal transition is associated with oxidative stress. In preterm infants, immaturity of the antioxidant system favours supplemental oxygen-derived morbidity and mortality. Objectives To assess if prolonging in utero-like oxygenation during the fetal-to-neonatal transition limits oxidative stress in the lung and brain, improving postnatal adaptation of mice pups. Material and methods Inspiratory oxygen fraction (FiO2) in pregnant mice was reduced from 21% (room air) to 14% (hypoxia) 8–12 h prior to delivery and reset to 21% 6–8 h after birth. The control group was kept at 21% during the procedure. Reduced (GSH) and oxidized (GSSG) glutathione and its precursors [γ-glut…

gsr (glutathione reductase gene)pgd phosphogluconate dehydrogenase geneGPX1FiO2 inspiratory oxygen fractionγ-GC (gamma-glutamyl cysteine)PhysiologyBiochemistryMice0302 clinical medicinePregnancyquinone oxidoreductase 1) [noq1 (NAD(P)H]NAD(P)H Dehydrogenase (Quinone)gapdh glyceraldehyde-3-phosphate dehydrogenase geneP7 1 week after birthGSH (reduced glutathione)Oxidoreductases Acting on Sulfur Group Donorsme1 (malic enzyme 1 gene)glutathioneLungSpO2 oxygen saturationlcsh:QH301-705.5γ-GC–NEM gamma-glutamyl cysteine covalently bonded to N-ethylmaleimidechemistry.chemical_classification0303 health sciencesGSSG oxidized glutathioneGlutathione peroxidaseO14 (hypoxia group FiO2=14%)Brainm/z mass-to-charge ratioG18 18th day of gestationCell Hypoxia3. Good healthpgd (phosphogluconate dehydrogenase gene)In uterogclm glutamylcysteine ligase modifier subunit genesrnx1 sulfiredoxin 1 genelcsh:Medicine (General)me1 malic enzyme 1 genesrnx1 (sulfiredoxin 1 gene)gclm (glutamylcysteine ligase modifier subunit gene)γ-GC–NEM (gamma-glutamyl cysteine covalently bonded to N-ethylmaleimide)trxnd1 (thioredoxin reductase 1 gene)redox regulation03 medical and health sciencesnoq1 NAD(P)H:quinone oxidoreductase 1γ-GC gamma-glutamyl cysteineCySH L-cysteinePregnancyg6pdx (glucose 6 phosphate dehydrogenase gene)GlutathioneOxygenationgapdh (glyceraldehyde-3-phosphate dehydrogenase gene)medicine.diseaseMice Inbred C57BLOxygenP1 24 h after birthGCL glutamylcysteine ligasechemistryOxidative stressRedox regulationNEM (N-ethylmaleimide)O14 hypoxia group (FiO2=14%)GSH reduced glutathioneClinical Biochemistrymedicine.disease_causechemistry.chemical_compoundGlutathione Peroxidase GPX1GS–NEM reduced glutathione covalently bonded to N-ethylmaleimideSpO2 (oxygen saturation)oxidative stressg6pdx glucose 6 phosphate dehydrogenase genelcsh:R5-920GSSG (oxidized glutathione)G18 (18th day of gestation)gsr glutathione reductase geneGlutathionegpx1 glutathione peroxidase 1 genemedicine.anatomical_structurem/z (mass-to-charge ratio)LC–MS/MS (liquid chromatography coupled to tandem mass spectrometry)FemaleLC–MS/MS liquid chromatography coupled to tandem mass spectrometryO21 (normoxia group FiO2=21%)paO2 (partial pressure of oxygen)gpx1 (glutathione peroxidase 1 gene)Research Papernoq1 (NAD(P)H:quinone oxidoreductase 1)CySH (l-cysteine)FiO2 (inspiratory oxygen fraction)CyS–NEM (cysteine covalently bonded to N-ethylmaleimide)030225 pediatricsmedicineP7 (1 week after birth)AnimalsGCL (glutamylcysteine ligase)P1 (24 h after birth)O21 normoxia group (FiO2=21%)CyS–NEM cysteine covalently bonded to N-ethylmaleimide030304 developmental biologyGlutathione PeroxidaseLungOrganic ChemistryGS–NEM (reduced glutathione covalently bonded to N-ethylmaleimide)trxnd1 thioredoxin reductase 1 geneMolecular biologypaO2 partial pressure of oxygenAnimals NewbornGene Expression Regulationlcsh:Biology (General)NEM N-ethylmaleimidefetal-to-neonatal transitionoxygenOxidative stressFetal-to-neonatal transition
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Conduction properties of thin films from a water soluble carbon nanotube/hemicellulose complex.

2018

We have examined the conductive properties of carbon nanotube based thin films, which were prepared via dispersion in water by non-covalent functionalization of the nanotubes with xylan, a type of hemicellulose. Measurements of low temperature conductivity, Kelvin probe force microscopy, and high frequency (THz) conductivity elucidated the intra-tube and inter-tube charge transport processes in this material. The measurements show excellent conductive properties of the as prepared thin films, with bulk conductivity up to 2000 S cm−1. The transport results demonstrate that the hemicellulose does not seriously interfere with the inter-tube conductance. peerReviewed

hemiselluloosaMaterials scienceconduction propertieshiiliXylan (coating)Bioengineering02 engineering and technologyCarbon nanotubeConductivity010402 general chemistry01 natural scienceslaw.inventionchemistry.chemical_compoundlawGeneral Materials ScienceHemicelluloseElectrical and Electronic EngineeringThin filmQCKelvin probe force microscopeksylaanitMechanical EngineeringGeneral Chemistry021001 nanoscience & nanotechnologyThermal conduction0104 chemical scienceschemistryChemical engineeringMechanics of Materialsohutkalvot0210 nano-technologyDispersion (chemistry)Nanotechnology
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Experimental studies on carbon nanotubes and graphene functionalized via physical adsorption with cellulose and avidin

2018

In this Thesis I have experimentally studied structural, electronic, and optical properties of hybrids of nanocarbon materials, carbon nanotubes (CNT) and graphene, and certain biomacromolecules. The latter are especially xylan, a type of hemicellulose, and avidin, an important protein. Complexes of CNT with hemicellulose are attractive because the hybrid material is soluble in water. The conductive transport properties of thin films of CNT /hemicellulose have been systematically studied with different experimental tools. These are low temperature DC conduction measurements, Kelvin probe microscopy, and optical conductivity measurements at terahertz frequencies. The results clearly indicate …

hemiselluloosaksylaanitelectronic propertygraphenesähköiset ominaisuudethemicelluloseoptiset ominaisuudetavidinnanorakenteetpintailmiötavidiinigrafeenicarbon nanotubeohutkalvotnanoputketadsorptiomakromolekyylitsurface functionalization
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Micelles of hyaluronic acid-hexadecylamine derivatives for ocular release of hydrophobic durgs

2016

The topical route is the ideal way to release drugs to the eye. Unfortunately, the low ocular drug bioavailability associated with this route of administration, makes not very efficient the treatment of several ocular diseases. Nowadays, polymeric micelles occupy a significant role in the field of ocular drug delivery thanks to the advantages that they offer in comparison with the administration of drugs in the free form. Indeed, polymeric micelles are suitable for delivering hydrophobic drugs and they seem to be very promising in ocular drug delivery for their high kinetic and thermodynamic stability. Also, micellar systems are able to give a controlled drug release and to act as absorptio…

hyaluronic acidpolymeric micelle
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Hydrophobin (HFBI): a potential fusion partner for one-step purification of recombinant proteins from insect cells

2008

Hydrophobins play an important role in binding and assembly of fungal surface structures as well as in medium-air interactions. These, hydrophobic properties provide interesting possibilities when purification of macromolecules is concerned. In aqueous micellar two-phase systems, based on surfactants, the water soluble hydrophobins are concentrated inside micellar structures and, thus, distributed to defined aqueous phases. This, one-step purification is attractive particularly when large-scale production of recombinant proteins is concerned. In the present study the hydrophobin HFBI of Trichoderma reesei was expressed as an N-terminal fusion with chicken avidin in baculovirus infected inse…

hydrophobinaqueous micellar two-phase system (AMTPS)HydrophobinRecombinant Fusion ProteinsBlotting Westernfluorescence scanning microscopy (FSM)SpodopteraMicellesurfactantslaw.inventionFungal ProteinsPulmonary surfactantlawprotein purificationProtein purificationAnimalsMicellesTrichoderma reeseiTrichodermaMicroscopy Confocalbiologytechnology industry and agricultureAvidinbiology.organism_classificationBiochemistryCytoplasmRecombinant DNAbiology.proteinBaculoviridaeBiotechnologyAvidinProtein Expression and Purification
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