Search results for "MICROSATELLITES"
showing 10 items of 72 documents
Lack of molluscan host diversity and the transmission of an emerging parasitic disease in Bolivia.
2001
Fasciolosis is a re-emerging parasitic disease that affects an increasing number of people in developing countries. The most severe endemic affects the Bolivian Altiplano, where the liver fluke (Fasciola hepatica) and its hermaphroditic snail host, Lymnaea truncatula, have been introduced from Europe. To achieve a better understanding of the epidemiological situation and the consequences of the colonization event of this invasive species, genetic analysis of Bolivian snail populations was needed. Here we compare the genetic diversity and population structure of snail samples from the Bolivian Altiplano with samples from the Old World at six polymorphic microsatellite loci. Whereas some vari…
Genetic variability in Peregrine falcon populations of the Western Palaearctic region
2018
We analyzed variation in ten polymorphic microsatellites and a portion of cytochrome b mitochondrial DNA in 4 populations of the Peregrine falcon (Falco peregrinus). living in northern and southern Italy. Spain and Czech Republic to assess species diversity in the poorly investigated Western Palearctic region. The Spanish population lives in the contact zone between F. peregrinus peregrinus and F. p. brookei. both the northern Italian and the Czech populations live within the range of F. p. peregrinus and the southern Italian is within the F. p. brookei. We added to our cytochrome b sequence dataset comprising 81 samples. previously published mitochondrial DNA sequences (n = 31) of English …
Patterns of genetic variability and habitat occupancy in Crepis triasii (Asteraceae) at different spatial scales: insights on evolutionary processes …
2012
International audience; Background and Aims Archipelagos are unique systems for studying evolutionary processes promoting diversification and speciation. The islands of the Mediterranean basin are major areas of plant richness, including a high proportion of narrow endemics. Many endemic plants are currently found in rocky habitats, showing varying patterns of habitat occupancy at different spatial scales throughout their range. The aim of the present study was to understand the impact of varying patterns of population distribution on genetic diversity and structure to shed light on demographic and evolutionary processes leading to population diversification in Crepis triasii, an endemic pl…
Genetic diversity of fig (Ficus caricaL.) genotypes grown in Southern Italy revealed by the use of SSR markers
2017
The genetic variability among 181 fig (Ficus carica L.) accessions found in small farms located in Campania, Basilicata, Apulia, Calabria and Sicily was investigated analysing the polymorphism of 18 simple sequence repeat (SSR) markers. The SSR analysis revealed a large genetic diversity among accessions. A total of 117 alleles were detected with a mean of 6.5 locus-1. The average expected (He) and observed heterozygosity (Ho) were 0.56 and 0.66, respectively. The mean polymorphic information content (PIC) was 0.51, suggesting a significant molecular diversity among the fig accessions taken into consideration. The UPGMA cluster analysis discriminated 174 genotypes and allowed to find 8 grou…
Identification of (In)Compatible S-genotypes and Molecular Characterisation of Italian Sweet Cherry Cultivars.
2017
Italy is the first sweet cherry producer in Europe. Its rich germplasm of local varieties has not been fully characterised or exploited in breeding programmes. Sweet cherry is a self-incompatible species; this trait is controlled by a gametophytically expressed multi-allelic (S) locus. The knowledge of sweet cherry S-alleles and cross-incompatibility groups is important for growers and breeders for choosing appropriate pollinators in the orchard and planning crosses. In this work, we analysed 94 sweet cherry cultivars native of large part of the Italian regions where cherry is grown. The microsatellite primer pair set and the reference cultivars agreed for cherry by the Prunus Working Group…
Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii
2019
Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite used to control whiteflies and thrips in protected crops. This biocontrol agent, originating from the Eastern Mediterranean region, has been mass-reared for commercial use since 2005 and is widely used in augmentative biocontrol programs. As a polyphagous predator, it has to cope with different biotic and abiotic factors. However, possible adaptation to mass rearing for production might be hindering its resilience and capacity for optimum performance in the field. In this study, we investigated the effect of long-term mass rearing on the genetic diversity of A. swirskii. We identified six microsatellite loci from w…
Brown trout (Salmo trutta L.) high genetic diversity around the Tyrrhenian Sea as revealed by nuclear and mitochondrial markers
2018
The brown trout (Salmo trutta L.) is widely distributed all around Europe but its natural diversity is threatened by massive stocking with Atlantic domestic strains. Describing the remaining natural genetic diversity and the proportion of domestic hatchery strains in rivers is a prerequisite for smart conservation. The high genetic diversity of brown trout populations around the Tyrrhenian Sea is well known. Use of twelve microsatellites has allowed description of the natural genetic structure of populations and detection of the consequences of stocking. Mitochondrial DNA control region sequences and the LDH-C1* gene enabled placement of each population into one of the six mitochondrial and…
Diversifying selection on MHC class I in the house sparrow (Passer domesticus).
2009
10 pages; International audience; Genes of the major histocompatibility complex (MHC) are the most polymorphic loci known in vertebrates. Two main hypotheses have been put forward to explain the maintenance of MHC diversity: pathogen-mediated selection and MHC-based mate choice. Host-parasite interactions can maintain MHC diversity via frequency-dependent selection, heterozygote advantage, and diversifying selection (spatially and/or temporally heterogeneous selection). In this study, we wished to investigate the nature of selection acting on the MHC class I across spatially structured populations of house sparrows (Passer domesticus) in France. To infer the nature of the selection, we comp…
Genetic homogeneity in the deep-sea grenadier Macrourus berglax across the North Atlantic Ocean
2018
Paucity of data on population structure and connectivity in deep sea species remains a major obstacle to their sustainable management and conservation in the face of ever increasing fisheries pressure and other forms of impacts on deep sea ecosystems. The roughhead grenadier Macrourus berglax presents all the classical characteristics of a deep sea species, such as slow growth and low fecundity, which make them particularly vulnerable to anthropogenic impact, due to their low resilience to change. In this study, the population structure of the roughhead grenadier is investigated throughout its geographic distribution using two sets of molecular markers: a partial sequence of the Control Reg…
Interbreeding between local and translocated populations of a cleaner fish in an experimental mesocosm predicts risk of disrupted local adaptation
2019
Source at https://doi.org/10.1002/ece3.5246. Translocation of organisms within or outside its native range carries the risk of modifying the community of the recipient ecosystems and induces gene flow between locally adapted populations or closely related species. In this study, we evaluated the genetic consequences of large‐scale translocation of cleaner wrasses that has become a common practice within the salmon aquaculture industry in northern Europe to combat sea lice infestation. A major concern with this practice is the potential for hybridization of escaped organisms with the local, recipient wrasse population, and thus potentially introduce exogenous alleles and breaking down coadap…