Search results for "MIDI"

Influence of double infections on the induction of thymidine kinase by UV-irradiated herpes simplex virus types 1 and 2 and pseudorabies virus

1975

Simultaneous infection of primary rabbit kidney cells with HSV type 1 TK+ and a TK- strain results in a mutual influence of both viruses on the induction of thymidine kinase (TK). TK+ virus has an enhancing and TK- virus a depressing effect on TK induction by a superinfecting TK+ virus. The enzyme induction depends on the ratio of multiplicities of both viruses. The mutual influence on TK induction depends further on the time of addition of the superinfecting virus: the effect of the second virus can still be observed when given 6 hours after primary infection. Identical phenomena can be observed using combinations with HSV type 2 or Pseudorabies viruses. The ability of HSV to induce TK is …

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

2014

Trehalose, a naturally occurring non-reducing disaccharide, is known to act as a major protein stabilizer that can reduce ultraviolet B (UVB)-induced corneal damage when topically applied to the eye. However, due to the low skin permeability of trehalose, which makes the development of topical formulations difficult, its use as a skin photoprotective agent has been limited. Previous findings demonstrated that liposomes may significantly improve the intracellular delivery of trehalose. Therefore, the present study aimed to assess the protective effects of trehalose-loaded liposomes against UVB-induced photodamage using the immortalized human keratinocyte cell line, HaCaT. The effects were al…

Come una spugna. Umidità del femminile nel pensiero medico e biologico della Grecia classica

2012

The article highlights how much humidity is constitutive of female physiology found in the gynecological works of Hippocrates and among Aristotelian biological thought, and shows the connections of this topic with other works of Hippocrates, as Victus and De aere. This female humidity, although ideologically constructed, is socially and politically indispensable, so that the complex therapy tends to the restoration of its right amount.

Test-retest and validity of a web-based food-frequency questionnaire for adolescents aged 13-14 to be used in the Norwegian Mother and Child Cohort S…

2014

Complete-active-space second-order perturbation theory (CASPT2//CASSCF) study of the dissociative electron attachment in canonical DNA nucleobases ca…

2015

Low-energy (0-3 eV) ballistic electrons originated during the irradiation of biological material can interact with DNA/RNA nucleobases yielding transient-anion species which undergo decompositions. Since the discovery that these reactions can eventually lead to strand breaking of the DNA chains, great efforts have been dedicated to their study. The main fragmentation at the 0-3 eV energy range is the ejection of a hydrogen atom from the specific nitrogen positions. In the present study, the methodological approach introduced in a previous work on uracil [I. González-Ramírez et al., J. Chem. Theory Comput. 8, 2769-2776 (2012)] is employed to study the DNA canonical nucleobases fragmentations…

Absolute Humidity Influences the Seasonal Persistence and Infectivity of Human Norovirus

2014

ABSTRACT Norovirus (NoV) is one of the main causative agents of acute gastroenteritis worldwide. In temperate climates, outbreaks peak during the winter season. The mechanism by which climatic factors influence the occurrence of NoV outbreaks is unknown. We hypothesized that humidity is linked to NoV seasonality. Human NoV is not cultivatable, so we used cultivatable murine norovirus (MNV) as a surrogate to study its persistence when exposed to various levels of relative humidity (RH) from low (10% RH) to saturated (100% RH) conditions at 9 and 25°C. In addition, we conducted similar experiments with virus-like particles (VLPs) from the predominant GII-4 norovirus and studied changes in bin…

Solvent-Enhanced Diastereo- and Regioselectivity in the PdII-Catalyzed Synthesis of Six- and Eight-Membered Heterocycles viacis-Aminopalladation

2009

The Pd(II)-catalyzed intramolecular oxidative cyclization of tosyl-protected cis- and trans-N-allyl-2-aminocyclohexanecarboxamides was examined, and efficient syntheses of cyclohexane-fused pyrimidin-4-ones and 1,5-diazocin-6-ones were developed. In the course of the research, a marked solvent effect was observed on both the regio- and diastereoselectivity. Additionally, a novel Pd(II)-mediated domino oxidation, oxidative amination reaction was discovered. Our experimental and theoretical findings suggest that the reactions proceed via a cis-aminopalladation mechanism.

Staphyloccocus epidermidis virulences faktoru un antibakteriālās rezistences noteikšana

2020

Darba aktualitāte: Neraugoties uz to, ka diagnosticēšanas un ārstniecības iespējas strauji attīstās, vēl joprojām aktuāla ir tēma par baktēriju rezistenci. S. epidermidis tiek uzskatīts par vienu no biežākajiem nazokomiālo infekciju ierosinātājiem. Šīs baktērijas svarīgākais virulences faktors ir biofilmas veidošanās. Ņemot vērā baktēriju īpašības, S. epidermidis izraisītās infekcijas kļūst grūti ārstējamas. Darba mērķis: Noteikt medicīnas studentu ādas mikroflorā (mikrobiota) esošos stafilokokus un identificēt S. epidermidis baktērijas, noteikt to virulences, antibakteriālo rezistenci un salīdzināt ar to stafilokoku celmu īpašībām, kas iegūti no klīniskā materiāla. Metodes un materiāli: Di…

Anti-inflammatory Function of High-Density Lipoproteins via Autophagy of IκB Kinase

2015

Background & Aims: Plasma levels of high-density lipoprotein (HDL) cholesterol are frequently found decreased in patients with inflammatory bowel disease (IBD). Therefore, and because HDL exerts anti-inflammatory activities, we investigated whether HDL and its major protein component apolipoprotein A-I (apoA-I) modulate mucosal inflammatory responses in vitro and in vivo. Methods: The human intestinal epithelial cell line T84 was used as the in vitro model for measuring the effects of HDL on the expression and secretion of tumor necrosis factor (TNF), interleukin-8 (IL-8), and intracellular adhesion molecule (ICAM). Nuclear factor-κB (NF-κB)-responsive promoter activity was studied by …

Prediction of conidial germination of Penicillium chrysogenum as influenced by temperature, water activity and pH.

2001

M. SAUTOUR, A. ROUGET, P. DANTIGNY, C. DIVIES AND M. BENSOUSSAN. 2001 Aims: Conidial germination of Penicillium chrysogenum was carried out under operating conditions compatible with a pastries manufacturing process. Methods and Results: A range, limited by two experimental values, was defined for each environmental factor tested: temperature (15 or 25°C), water activity (0·75 or 0·85) and pH (3·5 or 5·5). A closed device was made, which maintained an equilibrium between water activity of the culture medium and atmospheric relative humidity during 25 days, to follow spore germination. The combined effects of temperature, water activity and pH on spore germination were studied by applying fa…