Search results for "MORPHOLOGY"
showing 10 items of 1425 documents
P11.09 Pan-RTK inhibition of sLRIG1 mediates AXL downregulation in Glioblastoma
2019
Abstract INTRODUCTION Aberrant regulation of receptor tyrosine kinase (RTK) activity is characteristic of Glioblastoma (GBM). However, RTK-based targeted therapies have been largely unsuccessful in GBM patients, partially due to the complexity and redundance of RTK signaling. LRIG1 (Leucine-rich Repeats and ImmunoGlobulindomains protein 1) is known as an endogenous inhibitor of epidermal growth factor receptor (EGFR) during health and disease, however its mechanism of action is poorly understood. We previously showed that the soluble form of LRIG1 potently inhibits of GBM growth in vivo, irrespective of EGFR expression level and status, suggesting the involvement of other RTKs. Here, we aim…
Abstract 3100: Replacing fetal calf serum by human platelet lysate in cancer research and toxicology
2018
Abstract Experiments with cultured mammalian cells represent a common in vitro alternative to animal experiments. Fetal calf serum (FCS) is the most commonly used medium supplement. FCS contains a mixture of largely undefined growth factors and cytokines. Since FCS is received from unborn calves older than three months, it represents a massive burden for the pregnant cows and their fetuses. A needle is inserted into the heart of the fetus to collect blood. Since the animal is not under anesthesia, it may suffer pain and discomfort. Furthermore, the undefined nature of FCS is a source of experimental variation, undesired immune responses, and possible contaminations. Thus, alternative, defin…
Late steps of parvoviral infection induce changes in cell morphology.
2008
Previously, virus-induced non-filopodial extensions have not been encountered in connection with viral infections. Here, we report emergence of long extensions protruding from Norden laboratory feline kidney (NLFK) and A72 (canine fibroma) cells infected with canine parvovirus for 72 h. These extensions significantly differ in length and number from those appearing in control cells. The most striking feature in the extensions is the length, reaching up to 130 microm, almost twice the average length of a healthy NLFK cell. In A72 cells, the extensions were even longer, up to 200 microm. The results presented here also suggest that the events leading to the growth of these extensions start ea…
Type V collagen and protein kinase C η down-regulation in 8701-BC breast cancer cells
2011
We previously reported that ductal infiltrating carcinomas (d.i.c.) of the human breast display profound modifications of the stromal architecture, associated with anomalous collagen composition. Among the major alterations observed in the interstitial collagen, the relative increase of type V collagen content was detected. When type V collagen was used as an "in vitro" substrate for 8701-BC d.i.c. cells, it appeared able to restrain cell growth, inhibit cell motility and invasion "in vitro", and modify the expression levels of genes coding for apoptosis factors, caspases and stress response proteins. In the present paper we demonstrate that type V collagen induces the down-regulation of pr…
Gibberellic acid and flower bud development in loquat (Eriobotrya japonica Lindl.)
2011
The application of gibberellic acid (GA 3) to the whole loquat tree from mid-May to early June and from early August to the onset of flowering, significantly reduced the number of premature flowering shoots per current shoot and per m 3 of canopy, and so reduced the total number of panicles per m 3 of canopy. The number of vegetative shoots per m 3 of canopy was also significantly reduced by applying GA 3. The response depended on the concentration applied and produced optimal results at 50mgl -1. Differences in the number of flowers per panicle and leaves per shoot were not significantly modified by the treatment. Nevertheless, GA 3 applied directly to the developing apex near to flower di…
Preliminary data concerning the morphology of a Calabrian Ionian margin area: Caulonia and Marina di Gioiosa canyons
2008
In the framework of the Vector National Italian Project (VulCost line), aimed to study the role of the morphology and the geology of the Ionian Calabrian margin in the coastline evolution, an oceanographic cruise was planned to collect geophysical data along two canyon systems: Caulonia and Marina di Gioiosa. The survey explored the continental shelf and slope from a depth of 15 m to more than 1150 m, using Multibeam Echosounder to investigate the seafloor topography. This work provides an outline of the erosive feature of the slope, shaped mostly by seasonal river input and by the connection to the structural and geological characteristics of the margin, made interesting by a narrow shelf …
Submarine Mass Movements Along a Sediment Starved Margin: The Menorca Channel (Balearic Islands - Western Mediterranean)
2014
6th International Symposium on Submarine Mass Movements and Their Consequences (ISSMMTC), 23-25 September 2013, Kiel, Germany.-- 10 pages, 5 figures
Potential Cyclic Steps in a Gully System of the Gulf of Palermo (Southern Tyrrhenian Sea)
2016
Multibeam bathymetric data revealed the occurrence of atrain of bedforms along a gully system in the Gulf of Palermo, Southern Tyrrhenian Sea. The observed gullies, located in the westernmost sector of the Gulf of Palermo, incise the outer shelf at a depth of 120 m and converge at the Zafferano Canyon, connecting to the Palermo Basin at a the depth of 1300 m. Bedforms develop along these gullies and along the thalweg of the canyon, displaying an average wavelength of 200 m, with maximum values of 340 m. Their gully floor location combined with their wave length, upslope asymmetry and crescent shape point to a possible cyclic steporigin of these bedforms. Preliminary numerical modeling sugge…
One My scale subsidence of carbonate sedimentary bodies and the viscosity of the lower crust
2004
Abstract The possibility of flow of the lower crust under the load produced by carbonate sedimentary accumulations is investigated through the example of the Paris basin during the Middle Jurassic (i.e. Bathonian). Depositional geometries, water depths and sedimentary environments have been estimated and correlated for 164 sites spread over a surface of 380 per 220 km for three successive periods lasting each less than 0.8 My. A signal of relative vertical displacement has been extracted from water-depth and sedimentary thickness. Data have then been interpolated to produce maps of velocity of vertical displacement, sedimentation rate, water depth, and water-depth variation between two peri…
Highly porous novel chondro-instructive bioactive glass scaffolds tailored for cartilage tissue engineering
2021
Abstract Cartilage injuries remain challenging since the regenerative capacity of cartilage is extremely low. The aim was to design a novel type of bioactive glass (BG) scaffold with suitable topology that allows the formation of cartilage-specific extracellular matrix (ECM) after colonization with chondrogenic cells for cartilage repair. Highly porous scaffolds with interconnecting pores consisting of 100 % BG were manufactured using a melting, milling, sintering and leaching technique. Scaffolds were colonized with porcine articular chondrocytes (pAC) and undifferentiated human mesenchymal stromal cells (hMSC) for up to 35 days. Scaffolds displayed high cytocompatibility with no major pH …