Search results for "Making"
showing 10 items of 1218 documents
AcetyltransferaseSAS2and sirtuinSIR2,respectively, control flocculation and biofilm formation in wine yeast
2014
Cell-to-cell and cell-to-environment interactions of microorganisms are of substantial relevance for their biotechnological use. In the yeast Saccharomyces cerevisiae, flocculation can be an advantage to clarify final liquid products after fermentation, and biofilm formation may be relevant for the encapsulation of strains of interest. The adhesion properties of wine yeast strains can be modified by the genetic manipulation of transcriptional regulatory proteins, such as histone deacetylases, and acetylases. Sirtuin SIR2 is essential for the formation of mat structures, a kind of biofilm that requires the expression of cell-wall protein FLO11 as its deletion reduces FLO11 expression, and ad…
Study of the First Hours of Microvinification by the Use of Osmotic Stress-response Genes as Probes
2002
Summary When yeast cells are inoculated into grape must for vinification they find stress conditions because of osmolarity, which is due to very high sugar concentration, and pH lower than 4. In this work an analysis of the expression of three osmotic stress induced genes ( GPD1 , HSP12 and HSP104 ) under microvinification conditions is shown as a way to probe those stress situations and the regulatory mechanisms that control them. The results indicate that during the first hours of microvinification there is an increase in the GPD1 mRNA levels with a maximum about one hour after inoculation, and a decrease in the amount of HSP12 and HSP104 mRNAs, although with differences between them. The…
A new PCR-based method for monitoring inoculated wine fermentations.
2003
A new PCR-based method has been developed to monitor inoculated wine fermentations. The method is based on the variation in the number and position of introns in the mitochondrial gene COX1. Oligonucleotide primers homologous to the regions flanking the Saccharomyces cerevisiae COX1 introns have been designed and tested for S. cerevisiae wine yeast strain differentiation. Four primers were selected for their subsequent use in a multiplex PCR reaction and have proved to be very effective in uncovering polymorphism in natural and commercial yeast strains. An important point is that the speed and simplicity of the technique, which does not require the isolation of DNA, allows early detection o…
Design of a safety training package for migrant workers in the construction industry
2021
Abstract The construction sector is known as a high-risk sector with many safety challenges. It is also characterised by a large number of migrant workers and these workers report higher accident rates than native workers. This paper presents the design of the CSTP (Construction Safety Training Programme). The CSTP is a theory-based training program aimed at improving safety behaviours in construction sites acknowledging the particular challenges migrant workers face. Based on second and third generational models of training, we developed a training program that addresses the challenges faced by migrant workers in the construction industry, namely language and cultural barriers, times press…
Management of uncertain pairwise comparisons in AHP through probabilistic concepts
2019
Abstract Fast and judicious decision-making is paramount for the success of many activities and processes. However, various degrees of difficulty may affect the achievement of effective and optimal solutions. Decisions should ideally meet the best trade-off among as many of the involved factors as possible, especially in the case of complex problems. Substantial cognitive and technical skills are indispensable, while not always sufficient, to carry out optimal evaluations. One of the most common causes of wrong decisions derives from uncertainty and vagueness in making forecasts or attributing judgments. The literature shows numerous efforts towards the optimization and modeling of uncertai…
Knowledge exchange through science diplomacy to assist disaster risk reduction
2021
Abstract This paper analyses science diplomacy efforts to reduce disaster risks and proposes establishing national knowledge exchange centers (KECs) to help individual states adhere to their Sendai Framework goals. KECs are considered to be interconnected globally and work together to promote resilience efforts by facilitating sharing of information and strategies in risk monitoring, assessment, and ultimately reduction across the globe. KECs can provide high-quality scientific evidence for informed decisionmaking along with a component related to disaster science media to ensure that appropriate knowledge reaches a variety of people who need it in different forms tailored for them. KECs ca…
Enseñanza de las ciencias : revista de investigación y experiencias didácticas
2021
La biodiversidad es un concepto complejo, convertido en un importante referente curricular. Esta investigación explora si un ejercicio avanzado de las competencias necesarias para hacer significativo el concepto de biodiversidad en diferentes contextos conlleva una mejora significativa de la calidad de los argumentos implicados en la toma de decisiones sobre conservación de la biodiversidad. Para ello se ha aplicado una metodología cualitativa al análisis de doce argumentos formulados por estudiantes de 1.º de Bachillerato, caracterizados por su elevada complejidad estructural, de los cuales se muestra uno a modo de ejemplo. Los resultados revelan que, a pesar del nivel de cohesión estructu…
Assessment of wastewater treatment alternatives for small communities: An analytic network process approach
2015
The selection of the most appropriate wastewater treatment (WWT) technology is a complex problem since many alternatives are available and many criteria are involved in the decision-making process. To deal with this challenge, the analytic network process (ANP) is applied for the first time to rank a set of seven WWT technology set-ups for secondary treatment in small communities. A major advantage of ANP is that it incorporates interdependent relationships between elements. Results illustrated that extensive technologies, constructed wetlands and pond systems are the most preferred alternatives by WWT experts. The sensitivity analysis performed verified that the ranking of WWT alternatives…
Rilevazione, creazione, resistenza: l'opinione separata e il diritto internazionale
2022
Separate opinions have made the object of extensive scholarship in recent decades: in the international legal order, this is a consequence of that institution’s peculiar success, as virtually all judiciary and quasi-judiciary bodies allow for the allegation of separate opinions (either concurring or dissenting) to their rulings. This contribution tests the impact of separate opinions on subsequent case law and opinions, on the process of international law-making, and eventually on the mechanisms through which the Italian legal order adapts to international law as interpreted by international judiciary or quasi-judiciary bodies. The practice under scrutiny is admittedly multifarious; yet, it…
Fuzzy ServPerf model combined with ELECTRE III to comparatively evaluate service quality of international airports in Sicily
2015
In this paper a new approach was proposed so as to comparatively evaluate the quality of service alternatives. In particular, a fuzzy extension of the ServPerf service conceptual model was considered to estimate quality scores of fundamental service criteria, whereas the non-compensative multi-criteria decision-making ELECTRE III method was employed to point out the quality ranking of service alternatives on the basis of which the comparative service quality analysis was performed. In order to show the effectiveness of the proposed approach, an empirical study concerning service quality evaluation of the three international airports in Sicily (Italy) was conducted with detailed proposals fo…