Search results for "Matrix"
showing 10 items of 3205 documents
New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator
2008
In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two “rare” proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests…
Expanding the protein catalogue in the proteome reference map of human breast cancer cells
2006
In this report we present a catalogue of 162 proteins (including isoforms and variants) identified in a prototype of proteomic map of breast cancer cells. This work represents the prosecution of previous studies describing the protein complement of breast cancer cells of the line 8701-BC, which has been well characterized for several parameters, providing to be a useful model for the study of breast cancer-associated candidate biomarkers. In particular, 110 spots were identified ex novo by PMF, or validated following previous gel matching identification method; 30 were identified by N-terminal microsequencing and the remaining by gel matching with maps available from our former work. As a c…
Serum and antibodies of glaucoma patients lead to changes in the proteome, especially cell regulatory proteins, in retinal cells.
2012
PURPOSE: Previous studies show significantly specifically changed autoantibody reactions against retinal antigens in the serum of glaucoma and ocular hypertension (OHT) patients in comparison to healthy people. As pathogenesis of glaucoma still is unknown the aim of this study was to analyze if the serum and antibodies of glaucoma patients interact with neuroretinal cells. METHODS: R28 cells were incubated with serum of patients suffering from primary open angle glaucoma (POAG), normal tension glaucoma (NTG) or OHT, POAG serum after antibody removal and serum from healthy people for 48 h under a normal or an elevated pressure of 15000 Pa (112 mmHg). RGC5 cells were additionally incubated wi…
Multiple changes induced by fibroblasts on breast cancer cells.
2010
It is now widely recognised that the cross-talk between cancer and stromal cells may play a crucial role in cancer progression. However little is known about the complex underlying molecular mechanisms that occur within the tumor microenvironment. Fibroblasts are the major stromal cells with multiple roles, especially towards both the extracellular matrix and the neighbouring cell population, including neoplastic cells. Consequently, proteomic analyses would provide a wider resource for a better understanding of the potential modulating effects exerted by fibroblasts on cancer cells. In this report we describe the effects of fibroblast stimulation on the breast cancer cell line (8701-BC) pr…
Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangio…
2010
Certain proteins, including fibroblast growth factor-2 (FGF-2) and matrix metalloproteinase-9 (MMP-9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy in repairing damaged tissue. We provide the first evidence that mouse mesoangioblast stem cells release FGF-2 and MMP-9 in their active form through the production of membrane vesicles. These vesicles are produced and turned over continuously, but are stable for some time in the extracellular milieu. Mesoangioblasts shed membrane vesicles even under oxygen tensions that are lower than those typically used for cell culture and more like those of mouse tissues. These findings suggest that mesoangioblast…
The substrate degradome of meprin metalloproteases reveals an unexpected proteolytic link between meprin β and ADAM10
2012
The in vivo roles of meprin metalloproteases in pathophysiological conditions remain elusive. Substrates define protease roles. Therefore, to identify natural substrates for human meprin α and β we employed TAILS (terminal amine isotopic labeling of substrates), a proteomics approach that enriches for N-terminal peptides of proteins and cleavage fragments. Of the 151 new extracellular substrates we identified, it was notable that ADAM10 (a disintegrin and metalloprotease domain-containing protein 10)—the constitutive α-secretase—is activated by meprin β through cleavage of the propeptide. To validate this cleavage event, we expressed recombinant proADAM10 and after preincubation with meprin…
Finite-dimensional pseudo-bosons: a non-Hermitian version of the truncated harmonic oscillator
2018
We propose a deformed version of the commutation rule introduced in 1967 by Buchdahl to describe a particular model of the truncated harmonic oscillator. The rule we consider is defined on a $N$-dimensional Hilbert space $\Hil_N$, and produces two biorhogonal bases of $\Hil_N$ which are eigenstates of the Hamiltonians $h=\frac{1}{2}(q^2+p^2)$, and of its adjoint $h^\dagger$. Here $q$ and $p$ are non-Hermitian operators obeying $[q,p]=i(\1-Nk)$, where $k$ is a suitable orthogonal projection operator. These eigenstates are connected by ladder operators constructed out of $q$, $p$, $q^\dagger$ and $p^\dagger$. Some examples are discussed.
From pseudo-bosons to pseudo-Hermiticity via multiple generalized Bogoliubov transformations
2016
We consider the special type of pseudo-bosonic systems that can be mapped to standard bosons by means of generalized Bogoliubov transformation and demonstrate that a pseudo-Hermitian systems can be obtained from them by means of a second subsequent Bogoliubov transformation. We employ these operators in a simple model and study three different types of scenarios for the constraints on the model parameters giving rise to a Hermitian system, a pseudo-Hermitian system in which the second the Bogoliubov transformations is equivalent to the associated Dyson map and one in which we obtain D-quasi bases.
Modelling of Moisture Sorption by Cfrp Rebars with Vinylester Matrix
2004
The results of experimental investigation and modelling of moisture sorption by CFRP rebars and vinylester matrix are presented. Experimental values of the rebar diffusion coefficients essentially differ from those predicted using structural approach. Essential anisotropy of sorption process in longitudinal and transverse directions is observed. Quasi three phase model is suggested for description of the CFRP rebar sorption. The model is based on consideration of sorption process of the diffusion character in the composite consisted of two phases: resin and microplastic, which includes fibres and fibre-resin interphase. The suggested model describes the experimental sorption data quite sat…
Quando le cose vanno bene: analisi "terminabile" o "interminabile"? Le interruzioni e le conclusioni in psicoanalisi
2018
Criteria for the evaluation of the conclusion of the analytical path had already been dealt with by Freud (1937) in «Die endiiche und die unendiiche Analyse". In the following decades, with the change of theoretical assumptions and then with the new acquisitions in the field of Infant Research, which places the caregiver-child interaction as the foundation in the co-construction of the latter's personality, it became necessary to rethink the subject. This can be of great help to the psychotherapist, especially if the analysis is interrupted. In fact, even if the experience of unsuccessful therapeutic pathways can lead the analyst to a retrospective reflection on what happened during the the…