Search results for "Mcl-PHA"

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Draft genome sequence of Pseudomonas corrugata, a phytopathogenic bacterium with potential industrial applications

2014

Pseudomonas corrugata was first described as the causal agent of a tomato disease called 'pith necrosis' yet it is considered as a biological resource in various fields such as biocontrol of plant diseases and production of industrially promising microbial biopolymers (mcl-PHA). Here we report the first draft genome sequence of this species. © 2014 Elsevier B.V.

Mcl-PHAMolecular Sequence DataBiological pest controlBacterial ProteinBioengineeringPseudomonaApplied Microbiology and BiotechnologyBacterial proteinBacterial ProteinsSolanum lycopersicumBiocontrol agentPlant pathogenPseudomonasBotanyLycopersicon esculentumWhole genome sequencingBase SequencebiologyPseudomonasfood and beveragesSequence Analysis DNAGeneral Medicinebiology.organism_classificationCyclic lipopeptideQuorum sensingPseudomonas corrugataQuorum sensingPithGenome BacterialBacteriaBiotechnologyJournal of Biotechnology
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Transcriptional analysis of pha genes in pseudomonas mediterranea CFBP 5447 grown on glycerol

2014

We analysed the draft genome sequence of Pseudomonas mediterranea CFBP 5447 in order to identify firstly the central metabolic pathways that convert fatty acids or carbohydrate intermediates into mcl-PHA and secondly the genes involved in glycerol metabolism (glpF, glpK, glpD, glpR). Absence of the glpF gene, which codifies for the "glycerol uptake facilitator protein", was highlighted. In order to understand the expression of the pha gene cluster, we investigated the promoter activity of phaC1, phaC2, phaZ, phaD and phaI genes. When glycerol was present as the carbon source, PI was found to be the most active promoter. Expression analysis of the knock-out mutant of the phaD gene, which is …

Pseudomonas mediterraneamcl-PHA glycerol metabolismSettore AGR/12 - Patologia Vegetale
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