Search results for "Mechanism"
showing 10 items of 1835 documents
Pre-requisites of successful strategic electronic coordination : the moderation effect of the ownership mechanism of inter-organisational information…
2017
In this paper, we attempt to explain how the ownership mechanism of an interorganisational information system (IOS) may impact strategic information exchange (electronic coordination) induced by specific investments in the IOS. Recent research and practice show that heavy investments in IOSs demonstrate mixed results with respect to their impact on the electronic coordination. Consequently, the search of additional factors is needed to help and explain under what circumstances the IOS investments for strategic purposes become beneficial for the companies in a buyersupplier dyad. Transaction cost economics (TCE) and the hostage model are used as a framework for the research. 198 observations…
Aberrant gene expression profiles in Mediterranean sea urchin reproductive tissues after metal exposures
2019
Abstract Marine organisms are simultaneously exposed to numerous pollutants, among which metals probably represent the most abundant in marine environments. In order to evaluate the effects of metal exposure at molecular level in reproductive tissues, we profiled the sea urchin transcriptional response after non-lethal exposures using pathway-focused mRNA expression analyses. Herein, we show that exposures to relatively high concentrations of both essential and toxic metals hugely affected the gonadic expression of several genes involved in stress-response, detoxification, transcriptional and post-transcriptional regulation, without significant changes in gonadosomatic indices. Even though …
PCF11 links alternative polyadenylation to formation and spontaneous regression of neuroblastoma
2018
AbstractDiversification at the transcriptome 3’end is an important and evolutionarily conserved layer of gene regulation associated with differentiation and dedifferentiation processes. However the underlying mechanisms and functional consequences are poorly defined. Here, we identify extensive transcriptome-3’end-alterations in neuroblastoma, a tumour entity with a paucity of recurrent somatic mutations and an unusually high frequency of spontaneous regression. Utilising extensive RNAi-screening we reveal the landscape and drivers of transcriptome-3’end-diversification, discovering PCF11 as critical regulator, directing alternative polyadenylation (APA) of hundreds of transcripts including…
Optogenetics in Stem Cell Research: Focus on the Central Nervous System
2017
Stem cell-based therapies of CNS disorders represent a promising approach in translational and regenerative medicine. Stem cell-based tissue replacement and regeneration would, for the first time, offer a causal treatment strategy which is most likely not bound to a specific time window. Therapeutic strategies relying on this paradigm would require administration of exogenous stem cells to the CNS and/or the augmentation of endogenous stem cell capabilities. However, it remains unclear whether tissue replacement or bystander effects are required to induce such effects. Conventional experimental techniques will not be able to causally reveal such information, due to the complexity and coinci…
Prediction of lncRNA-Disease Associations from Tripartite Graphs
2021
The discovery of novel lncRNA-disease associations may provide valuable input to the understanding of disease mechanisms at lncRNA level, as well as to the detection of biomarkers for disease diagnosis, treatment, prognosis and prevention. Unfortunately, due to costs and time complexity, the number of possible disease-related lncRNAs verified by traditional biological experiments is very limited. Computational approaches for the prediction of potential disease-lncRNA associations can effectively decrease time and cost of biological experiments. We propose an approach for the prediction of lncRNA-disease associations based on neighborhood analysis performed on a tripartite graph, built upon …
The Patellofemoral Arthroplasty: The Great Solution, the Great Problem
2012
Unfortunately, many orthopedic surgeons believe that the definite solution for eliminating anterior knee pain in a young patient with multiple operations is the patellofemoral prosthesis (“The Great Solution”). But, they fail to ask themselves the most important question; what is causing the pain? When a patellofemoral prosthesis is implanted in these young patients without finding out the cause of the pain, a more serious problem could be created with a worse solution (“The Great Problem”). The cases we present spotlight this abuse.
Acidic Environment Leads to ROS-Induced MAPK Signaling in Cancer Cells
2011
Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e), pH(i)) and mitogen-activated-protein-kinases (ERK1/2, p38) was analyzed. Data were obtained mainly in AT1 R-3327 prostate carcinoma cells, but the principle importance was confirmed in 5 other cell types. Extracellular acidosis leads to a rapid and sustained decrease of pH(i) in parallel to p38 phosphorylation in all cell types and to ERK1/2 phosphorylation in 3 of 6 cell types. Furth…
NMR structure of a non-conjugatable, ADP-ribosylation associated, ubiquitin-like domain from Tetrahymena thermophila polyubiquitin locus.
2019
Abstract Background Ubiquitin-like domains (UbLs), in addition to being post-translationally conjugated to the target through the E1-E2-E3 enzymatic cascade, can be translated as a part of the protein they ought to regulate. As integral UbLs coexist with the rest of the protein, their structural properties can differ from canonical ubiquitin, depending on the protein context and how they interact with it. In this work, we investigate T.th-ubl5, a UbL present in a polyubiquitin locus of Tetrahymena thermophila, which is integral to an ADP-ribosyl transferase protein. Only one other co-occurrence of these two domains within the same protein has been reported. Methods NMR, multiple sequence al…
Angular momentum population in fragmentation reactions
2009
Neutron-deficient nuclei with N = 126 have been populated following projectile fragmentation of a 238U beam with energy 1 GeV/A. The decay of several previously reported isomers has been measured. This will allow us to calculate high-spin isomeric ratios and compare them with model calculations to allow a better understanding of the reaction mechanism. Algora, Alejandro, Alejandro.Algora@ific.uv.es ; Molina Palacios, Francisco Manuel, Francisco.Molina@ific.uv.es ; Rubio Barroso, Berta, Berta.Rubio@ific.uv.es
Unit 1. Methods to study organic reaction mechanisms
2018
Este documento ha sido elaborado en el marco de un proyecto de Renovación de Metodologías Docentes concedido por el Servicio de Formación Permanente de la Universitat de València (Código de la solicitud: UV-SFPIE_RMD17-725369) Este documento forma parte del temario de la asignatura "Química Orgánica Avanzada" del Máster Universitario en Química de la Universitat de València This document is part of the course "Advanced Organic Chemistry" of the Master's Degree in Chemistry of the University of Valencia