Search results for "Melanogaster"
showing 10 items of 452 documents
Molecular Basis for the Regulation of Cell Fate by the Lethal (2) Giant Larvae Tumour Suppressor Gene of Drosophila Melanogaster
2007
Tumour suppressor genes act as recessive determinants of cancer. Their function is required for normal cell growth and differentiation during development. When both alleles of these developmental genes are inactivated, cell growth becomes unrestricted. In Drosophila, a series of genes have been identified which when mutated produce tissue-specific tumours. Of these the lethal(2)giant larvae (l(2)gl) gene is the best studied. Homozygous l(2)gl mutations cause the development of malignant tumours in the brain and the imaginal discs. Genomic DNA from the l(2)gl locus has been cloned, introduced back into l(2)gl mutant animals by P-element-mediated transformation and shown to restore normal dev…
A luminal glycoprotein drives dose-dependent diameter expansion of the Drosophila melanogaster hindgut tube
2012
An important step in epithelial organ development is size maturation of the organ lumen to attain correct dimensions. Here we show that the regulated expression of Tenectin (Tnc) is critical to shape the Drosophila melanogaster hindgut tube. Tnc is a secreted protein that fills the embryonic hindgut lumen during tube diameter expansion. Inside the lumen, Tnc contributes to detectable O-Glycans and forms a dense striated matrix. Loss of tnc causes a narrow hindgut tube, while Tnc over-expression drives tube dilation in a dose-dependent manner. Cellular analyses show that luminal accumulation of Tnc causes an increase in inner and outer tube diameter, and cell flattening within the tube wall,…
Innovative and Applied Research in Biology: Proceedings, Vol. 3/2021
2021
The collection contains SCIENTIFIC articles on the topics of the LU 79 conference reports. The main focus is on innovative and applied research in biology and interdisciplinary fields.
Increased autophagy and apoptosis contribute to muscle atrophy in a myotonic dystrophy type 1 Drosophila model
2015
ABSTRACT Muscle mass wasting is one of the most debilitating symptoms of myotonic dystrophy type 1 (DM1) disease, ultimately leading to immobility, respiratory defects, dysarthria, dysphagia and death in advanced stages of the disease. In order to study the molecular mechanisms leading to the degenerative loss of adult muscle tissue in DM1, we generated an inducible Drosophila model of expanded CTG trinucleotide repeat toxicity that resembles an adult-onset form of the disease. Heat-shock induced expression of 480 CUG repeats in adult flies resulted in a reduction in the area of the indirect flight muscles. In these model flies, reduction of muscle area was concomitant with increased apopto…
cis-regulatory variation modulates susceptibility to enteric infection in the Drosophila genetic reference panel
2020
Abstract Background Resistance to enteric pathogens is a complex trait at the crossroads of multiple biological processes. We have previously shown in the Drosophila Genetic Reference Panel (DGRP) that resistance to infection is highly heritable, but our understanding of how the effects of genetic variants affect different molecular mechanisms to determine gut immunocompetence is still limited. Results To address this, we perform a systems genetics analysis of the gut transcriptomes from 38 DGRP lines that were orally infected with Pseudomonas entomophila. We identify a large number of condition-specific, expression quantitative trait loci (local-eQTLs) with infection-specific ones located …
Software/Hardware Issues in Modelling Insect Brain Architecture
2011
The concept of cognitive abilities is commonly associated to humans and animals like mammals, birds and others. Nevertheless, in the last years several research groups have intensified the studies on insects that posses a much simpler brain structure even if they are able to show interesting memory and learning capabilities. In this paper a survey on some key results obtained in a joint research activity among Engineers and Neurogeneticians is reported. They were focussed toward the design and implementation of a model of the insect brain inspired by the Drosophila melanogaster. Particular attention was paid to the main neural centers the Mushroom Bodies and the Central Complex. Moreover a …
Sex-specific effects of natural and sexual selection on the evolution of life span and ageing in Drosophila simulans
2014
Summary Variation in the strength of age-dependent natural selection shapes differences in ageing rates across species and populations. Likewise, sexual selection can promote divergent patterns of senescence across the sexes. However, the effects of these processes on the evolution of ageing have largely been considered independently, and interactions between them are poorly understood. We use experimental evolution to investigate how natural and sexual selection affect life span and ageing in Drosophila simulans. Replicate populations were evolved under lifetime monogamy (relaxed sexual selection) or lifetime polyandry (elevated sexual selection) and at one of two temperatures, 25 °C (rela…
Derepressing muscleblind expression by miRNA sponges ameliorates myotonic dystrophy-like phenotypes in Drosophila
2016
AbstractMyotonic Dystrophy type 1 (DM1) originates from alleles of the DMPK gene with hundreds of extra CTG repeats in the 3′ untranslated region (3′ UTR). CUG repeat RNAs accumulate in foci that sequester Muscleblind-like (MBNL) proteins away from their functional target transcripts. Endogenous upregulation of MBNL proteins is, thus, a potential therapeutic approach to DM1. Here we identify two miRNAs, dme-miR-277 and dme-miR-304, that differentially regulate muscleblind RNA isoforms in miRNA sensor constructs. We also show that their sequestration by sponge constructs derepresses endogenous muscleblind not only in a wild type background but also in a DM1 Drosophila model expressing non-co…
Function of odorant-binding proteins in the Drosophila melanogaster chemoreception
2017
National audience; Function of odorant-binding proteins in the Drosophila [i]melanogaster[/i] chemoreception. 18. rencontre du Club de neurobiologie des invertébrés
Desarrollo de modelos experimentales de LGMDD2 y rastreo de fármacos
2023
La distrofia muscular de cinturas tipo D2 (Limb-girdle muscular dystrophy type D2, LGMDD2) es una enfermedad genética ultra rara para la cual no existe tratamiento. Las principales características clínicas de la LGMDD2 son la debilidad y degeneración muscular progresiva que afecta a las cinturas pélvica y escapular de forma predominante. En 2013 se identificó la LGMDD2 como una miopatía autosómica dominante causada por una mutación en el gen de la transportina 3 (TNPO3), dando como resultado una proteína mutante con una extensión de 15 aminoácidos adicionales: TNPO3mut. TNPO3 es una β-importina responsable de la importación nuclear de proteínas ricas en serina/arginina (proteínas SR), invol…