Search results for "Melon"

Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV)

2007

AbstractCell-to-cell movement of the Melon Necrotic Spot Virus (MNSV) is controlled by two small proteins working in trans, an RNA-binding protein (p7A) and an integral membrane protein (p7B) separated by an amber stop codon. p7B contains a single hydrophobic region. Membrane integration of this region was observed when inserted into model proteins in the presence of microsomal membranes. Furthermore, we explored the topology and targeting mechanisms of full-length p7B. Here we present evidence that p7B integrates in vitro into the ER membrane cotranslationally and with an Nt-cytoplasmic/Ct-luminal orientation. The observed topology was monitored in vivo by fusing GFP to the Ct of p7B, enab…

Study and characterization of polyphenol oxidase from eggplant (Solanum melongena L.)

2011

ABSTRACT: In this study the catecholase and cresolase activities of eggplant polyphenol oxidase (PPO) were investigated. Enzyme activity was determined by measuring the increase in absorbance using catechol as substrate and 3-methyl-2- benzothiazolinone hydrazone (MBTH) as coupled reagent. The effects of substrate specificity, heat inactivation, temperature, pH, and inhibitors were investigated to understand the enzymatic alteration of ready-to-eat preparations. Browning of vegetables was determined through a colorimeter. Decrease of lightness (L*) and increase of color difference values (ΔE*) were correlated with tissue browning. Antibrowning agents were tested on PPO under the same condit…

Polyethylene and Biodegradable Starch-Based Mulching Films Positively Affect Winter Melon Production in Sicily

2014

Plastic mulching is widely used in many regions of the world for growing melons. The winter melon is largely grown in the hilly countryside of the Mediterranean island of Sicily (Italy) where mulching plastic films enhance early and total yields. However, removing and disposal of polyethylene plastic mulches after use represent a major disadvantage and an environmental concern. The objective of this trial was to compare the effects of polyethylene (PE) (transparent and black) and biodegradable (black) mulching films on yield, fruit size and quality of melon grown in the open field. The trial was conducted in the north-western coast of Sicily in the province of Trapani, 250-m elevation. Melo…

Abeilles et plantes cultivées en Espagne : actualisation des données pour le melon, la pastèque et l’amandier

2021

[EN] Pollinators of most of the plants cultivated in the Mediterranean regions of Europe are still unknown. We provide new data and we review previously available information on bees (Apoidea) associated with three economically important crops in Spain: melon (Cucumis melo L.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and almond (Prunus dulcis (Mill.) D.A.Webb). We found that, overall, 98 bee species spanning four families visit flowers of the studied crop species, and 46 additional species were sampled within the crops with pan-traps. The bee assemblages visiting melon include 7–33 species, with moderate to high importance of small Lasioglossum (Halictidae) and of honey bee …

Effects of Increasing Doses of Nitrogen Fertilization on Winter Melon Production in the Western Coast of Sicily

2010

The winter melon is largely grown in the open field in the Mediterranean island of Sicily on about 6000 hectares. Nitrogen fertilization is a key aspect of a winter melon cultural system as it can dramatically improve fruit production and play a crucial role on fruit quality attributes such as fruit size, colour and uniformity. An experiment was performed to determine the influence of increasing doses of nitrogen fertilization on fruit yield and quality of winter melon. The trial was conducted in the north-western coast of Sicily near Buseto Palizzolo in the province of Trapani, 250-m elevation. Plug type transplants cv. Helios were planted in the first week of May. A transparent polyethyle…

Combined Stress Conditions in Melon Induce Non-additive Effects in the Core miRNA Regulatory Network

2021

Climate change has been associated with a higher incidence of combined adverse environmental conditions that can promote a significant decrease in crop productivity. However, knowledge on how a combination of stresses might affect plant development is still scarce. MicroRNAs (miRNAs) have been proposed as potential targets for improving crop productivity. Here, we have combined deep-sequencing, computational characterization of responsive miRNAs and validation of their regulatory role in a comprehensive analysis of response of melon to several combinations of four stresses (cold, salinity, short day, and infection with a fungus). Twenty-two miRNA families responding to double and/or triple …

PARASITOID COMPLEX OF LIRIOMYZA BRYONIAE (KALTENBACH) (DIPTERA: AGROMYZIDAE) ON WINTER MELON (CUCUMIS MELO L. VAR. INODORUS) IN NORTH-WESTERN SICILY.

2002

Effetti delle eopche d'impianto sulla produttività del melone invernale "Ecotipo Purceddu"

2008

RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins

2006

AbstractAdvances in structural and biochemical properties of carmovirus movement proteins (MPs) have only been obtained in p7 and p9 from Carnation mottle virus (CarMV). Alignment of carmovirus MPs revealed a low conservation of amino acid identity but interestingly, similarity was elevated in regions associated with the functional secondary structure elements reported for CarMV which were conserved in all studied proteins. Nevertheless, some differential features in relation with CarMV MPs were identified in those from Melon necrotic virus (MNSV) (p7A and p7B). p7A was a soluble non-sequence specific RNA-binding protein, but unlike CarMV p7, its central region alone could not account for t…

Morphological and agronomical characterization of eggplant genetic resources from the Sicily area

2013

The eggplant is a vegetable crop widely grown throughout Sicily both in greenhouse and open field. This study was carried out by the Department of Agri-Environmental Systems at the University of Palermo in the Spring/Summer of 2011 in open fields. The aim of the study was to characterize 6 eggplant ecotypes (G1-G6) and three eggplant varieties (Birgah, Black bell and Viola di Firenze) from a morphological, phenological and production point of view, gathered from Sicily and the smaller islands. The genotypes G1 and G3 were found to be more productive than the varieties used in the test field. Ecotype G1 produced fruits which were dark violet and highly glossy, and produced the lowest percent…