Search results for "MetS"

FT-IR spectroscopy : A powerful tool for studying the inter- and intraspecific biodiversity of cultivable non-Saccharomyces yeasts isolated from grap…

2016

International audience; The efficiency of the FT-IR technique for studying the inter- and intra biodiversity of cultivable non-Saccharomyces yeasts (NS) present in different must samples was examined. In first, the capacity of the technique FF-IR to study the global diversity of a given sample was compared to the pyrosequencing method, used as a reference technique. Seven different genera (Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Issatchenkia, Metschnikowia and Pichia) were identified by FT-IR and also by pyrosequencing. Thirty-eight other genera were identified by pyrosequencing, but together they represented less than 6% of the average total population of 6 musts. Among the sp…

Exposure to environmental radionuclides alters mitochondrial DNA maintenance in a wild rodent

2020

AbstractMitochondria are sensitive to oxidative stress, including that derived from ionizing radiation. To quantify the effects of exposure to environmental radionuclides on mitochondrial DNA (mtDNA) dynamics in wildlife, bank voles (Myodes glareolus) were collected from the chernobyl exclusion zone (CEZ), where animals are exposed to elevated levels of radionuclides, and from uncontaminated areas within the CEZ and elsewhere in Ukraine. Brains of bank voles from outside the CEZ were characterized by low mtDNA copy number and low mtDNA damage; by contrast, bank voles within the CEZ had high mtDNA copy number and high mtDNA damage, consistent with putative damaging effects of elevated radiat…

Influence of freezing temperatures prior to freeze-drying on viability of yeasts and lactic acid bacteria isolated from wine

2017

Aims To determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (LAB). To know if a similar freeze-drying protocol can be used for both micro-organisms. Methods and Results Cells from liquid culture media were recovered and concentrated in appropriate lyoprotectants. Aliquots of each strain were frozen at −20, −80 and −196°C before vacuum drying. Viable cell counts were done before freezing and after freeze-drying. Survival rates were calculated. Freezing temperatures differently affected yeast and bacteria survival. The highest survival rates were obtained at −20 and −80°C for yeasts, but at −196°C for LAB. …

Socio-Reproductive Conflicts and the Father’s Curse Dilemma

2018

Evolutionary conflicts between males and females can manifest over sexually antagonistic interactions at loci or over sexually antagonistic interests within a locus. The latter form of conflict, intralocus sexual conflict, arises from sexually antagonistic selection and constrains the fitness of individuals through a phenotypic compromise. These conflicts, and socio-reproductive interactions in general, are commonly mediated by hormones, and thus predictive insights can be gained from studying their mediating effects. Here, we integrate several lines of evidence to describe a novel, hormonally mediated reproductive dilemma that we call the father’s curse, which results from an intralocus co…

Skin and gut microbiomes of a wild mammal respond to different environmental cues

2018

Background Animal skin and gut microbiomes are important components of host fitness. However, the processes that shape the microbiomes of wildlife are poorly understood, particularly with regard to exposure to environmental contaminants. We used 16S rRNA amplicon sequencing to quantify how exposure to radionuclides impacts the skin and gut microbiota of a small mammal, the bank vole Myodes glareolus, inhabiting areas within and outside the Chernobyl Exclusion Zone (CEZ), Ukraine. Results Skin microbiomes of male bank voles were more diverse than females. However, the most pronounced differences in skin microbiomes occurred at a larger spatial scale, with higher alpha diversity in the skin m…

Native Vineyard Non-Saccharomyces Yeasts Used for Biological Control of Botrytis cinerea in Stored Table Grape

2021

Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses of fresh fruit quality and vegetables during storage, distribution, and consumption. The current control strategy is the use of SO2 generator pads whose application is now largely under observation. A high quantity of SO2 can be deleterious for fresh fruits and vegetables and it is not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological approaches devoid of chemicals. In this direction, we carried out our study in isolating five different non-Saccharomyces yeast strains from local vineyards…

2017

Previous studies reported that the use of Metschnikowia pulcherrima in sequential culture fermentation with Saccharomyces cerevisiae mainly induced a reduction of volatile acidity in wine. The impact of the presence of this yeast on the metabolic pathway involved in pyruvate dehydrogenase (PDH) bypass and glycerol production in S. cerevisiae has never been investigated. In this work, we compared acetic acid and glycerol production kinetics between pure S. cerevisiae culture and its sequential culture with M. pulcherrima during alcoholic fermentation. In parallel, the expression levels of the principal genes involved in PDH bypass and glyceropyruvic fermentation in S. cerevisiae were investi…

Microbial Succession in Spontaneously Fermented Grape Must Before, During and After Stuck Fermentation

2016

The microbial succession in spontaneously fermenting Riesling must was investigated from the beginning (pressing) until the end (sulphuring) of the fermentation in two harvest years (2008 and 2009) at a Moselle winery (Germany). In both years, the fermentation was interrupted by a stuck period. The length of the stuck period varied considerably (20 weeks in 2008 and one week in 2009). Different yeasts (Candida, Debaryomyces, Pichia, Hanseniaspora, Saccharomyces, Metschnikowia, Cryptococcus, Filobasidium and Rhodotorula) and bacteria (Gluconobacter, Asaia, Acetobacter, Oenococcus, Lactobacillus, Bacillus and Paenibacillus) were isolated successively by plating. The main fermenting organism w…

Soiden ennallistamisen suoluonto-, vesistö-, ja ilmastovaikutukset. Vertaisarvioitu raportti.

2021

Tämän selvityksen tarkoituksena on tuottaa kirjallisuuskatsaus ja siihen pohjautuvia johtopäätöksiä soiden ennallistamisen ilmasto-, vesistö- ja suoluontovaikutuksista. Selvitys pyrkii erityisesti kasaamaan yhteen uusimpia tutkimustuloksia ja aineistoja ennallistamisen vaikutuksiin liittyen ja toisaalta muodostamaan kokonaiskuvan laaja-alaisempaa soiden ennallistamisstrategiaa silmällä pitäen. Suoekosysteemit reagoivat kokonai-suutena varsin hyvin perinteisiin ennallistamistoimiin. Ennallistaminen ei kuitenkaan ole ongelmatonta, sillä ennallistamistoimet eli yleensä kaivin- ja metsäkonetyöt sekä ennallistamisen jälkeisen alkuvaiheen voimakkaat ekosysteemin muutokset voivat aiheuttaa yhteisk…

Soiden ennallistamisen suoluonto-, vesistö- ja ilmastovaikutukset : Luontopaneelin yhteenveto ja suositukset luontopolitiikan suunnittelun ja päätöks…

2021

Suomen alkuperäisestä 10,4 miljoonan hehtaarin suoalasta yli puolet on ojitettu metsä- ja maatalouden sekä turvetuotannon tarpeisiin. Etelä-Suomessa ojitus on ollut voimakkainta: keskimäärin noin 75 prosenttia ja monin paikoin vielä suurempi osa soista on ojitettu. Suot ovat Euroopan luontotyypeistä kaikkein uhanalaisin luontotyyppiryhmä ja Suomella on erityisvastuu soiden suojelusta. Kaikkiaan 54 prosenttia Suomen 50 suoluontotyypistä on uhanalaisia ja lisäksi 20 prosenttia on silmällä-pidettäviä. Ensisijaisesti Suomen soilla elävistä lajeista 11 prosenttia eli yhteensä 120 lajia on uhanalaisia. Uhanalaisilla lajeilla ja luontotyypeillä on korkea riski hävitä Suomesta. Mittava ojitus näkyy…