Search results for "Methane"
showing 10 items of 1763 documents
Co-digestion of harvested microalgae and primary sludge in a mesophilic anaerobic membrane bioreactor (AnMBR): Methane potential and microbial divers…
2020
Abstract Anaerobic co-digestion of primary sludge and raw microalgae (Scenedesmus and Chlorella) was performed in a lab-scale semi-continuous anaerobic membrane bioreactor to assess the biological performance and identify the microbial community involved in the co-digestion process. The reactor was operated at 35 °C for 440 days, working at a solids retention time of 100 days. The system achieved 73% biodegradability and showed high stability in terms of pH and volatile fatty acids. An enriched microbial community was observed. Of the several phyla, Chloroflexi and Proteobacteria were the most abundant. Cellulose-degraders phyla (Bacteroidetes, Chloroflexi and Thermotogae) were detected. Sy…
Anaerobic membrane bioreactors (AnMBR) treating urban wastewater in mild climates
2020
[EN] Feasibility of an AnMBR demonstration plant treating urban wastewater (UWW) at temperatures around 25-30 degrees C was assessed during a 350-day experimental period. The plant was fed with the effluent from the pretreatment of a full-scale municipal WWTP, characterized by high COD and sulfate concentrations. Biodegradability of the UWW reached values up to 87%, although a portion of the biodegradable COD was consumed by sulfate reducing organisms. Effluent COD remained below effluent discharge limits, achieving COD removals above 90%. System operation resulted in a reduction of sludge production of 36-58% compared to theoretical aerobic sludge productions. The membranes were operated a…
Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel.
2019
Abstract Background Among the third-generation biodiesel feed stock, oleaginous marine yeasts are the least studied microorganisms for such purpose. Results Wild strains yeasts were isolated from various Tunisian marine sources including fish waste (Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015, Trichosporon asahii TaTun15 and Yarrowia lipolytica YlTun15) and seawater (Rhodotorula mucilaginosa RmTun15). Following incubation with ethyl methanesulfonate (EMS: 75 mM) for various periods of time (T15, T30, T45, T60 min), the cell viability of these strains responded differentially according to yeast species. For instance, mutated CtTun15 did not survive after 30 min of EMS treatment; …
Spatiotemporal patterns in methane flux and gas transfer velocity at low wind speeds: Implications for upscaling studies on small lakes
2016
Lakes contribute significantly to the global natural emissions of methane (CH4) and carbon dioxide. However, to accurately incorporate them into the continental carbon balance more detailed surveys of lacustrine greenhouse gas emissions are needed, especially in respect to spatiotemporal variability and to how this affects the upscaling of results. We investigated CH4 flux from a small, wind-shielded lake during 10 field trips over a 14 month period. We show that floating chambers may be used to calibrate the relationship between gas transfer velocity (k) and wind speed at 10 m height (U10) to the local system, in order to obtain more accurate estimates of diffusive CH4 flux than by applyin…
Assessing Methane Emission and Economic Viability of Energy Exploitation in a Typical Sicilian Municipal Solid Waste Landfill
2018
Sanitary landfills for municipal solid waste (MSW) represent one of the major anthropogenic source of GHGs emissions and are directly responsible of the climate changes we are facing nowadays. Indeed, the biodegradable organic matter of MSW undergoes anaerobic digestion producing the landfill gas (LFG), whose main components are CH4 and CO2. Therefore, biomethane energy exploitation in MSW landfills will reduce GHGs emission positively affecting the global warming. The aim of the present study was to assess the methane production in a Sicilian landfill by comparing the results from field measurements of methane emission and the estimates achieved by applying different mathematical models. A…
Acclimatised rumen culture for raw microalgae conversion into biogas: Linking microbial community structure and operational parameters in anaerobic m…
2019
[EN] Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spirochaetaceae, Lentimicrobiaceae and Cloacimonetes fermentative and hydrolytic members. During the highest biodegradability achieved in the AnMBR (62%) the dominant microorganisms were Fervidobacterium and Methanosaeta. Different microbial community clusters were observed at different SRT conditions. Interestingly, syntrophic bacteria Gelria and …
Carbon metabolic rates and GHG emissions in different wetland types of the Ebro Delta
2020
Deltaic wetlands are highly productive ecosystems, which characteristically can act as C-sinks. However, they are among the most threatened ecosystems, being very vulnerable to global change, and require special attention towards its conservation. Knowing their climate change mitigating potential, conservation measures should also be oriented with a climatic approach, to strengthen their regulatory services. In this work we studied the carbon biogeochemistry and the specific relevance of certain microbial guilds on carbon metabolisms of the three main types of deltaic wetlands located in the Ebro Delta, north-eastern Spain, as well as how they deal with human pressures and climate change ef…
Anaerobic oxidation of methane in sediments of a nitrate-rich, oligo-mesotrophic boreal lake
2021
AbstractThe identity of electron acceptors in promoting anaerobic oxidation of methane (AOM) in the sediments of boreal lakes is currently unknown. Here, we studied the AOM rate of sediment slurries collected from three profundal stations of a nitrate-rich, oligo-mesotrophic, boreal lake (Lake Pääjärvi, Finland), under varying nitrate concentrations using 13C-labelling. Furthermore, vertical profiles of the sediment and porewater geochemistry, and the microbial communities (16S rRNA gene and shotgun metagenomic sequencing) were analyzed. Despite geochemical data indicating that simultaneous consumption of nitrate and methane took place at the sediment layers chosen for incubations, AOM rate…
Ecosystem carbon response of an Arctic peatland to simulated permafrost thaw
2019
Permafrost peatlands are biogeochemical hot spots in the Arctic as they store vast amounts of carbon. Permafrost thaw could release part of these long-term immobile carbon stocks as the greenhouse gases (GHGs) carbon dioxide (CO 2 ) and methane (CH 4 ) to the atmosphere, but how much, at which time-span and as which gaseous carbon species is still highly uncertain. Here we assess the effect of permafrost thaw on GHG dynamics under different moisture and vegetation scenarios in a permafrost peatland. A novel experimental approach using intact plant–soil systems (mesocosms) allowed us to simulate permafrost thaw under near-natural conditions. We monitored GHG flux dynamics via high-resolution…
Carbon emission along a eutrophication gradient in temperate riverine wetlands: effect of primary productivity and plant community composition
2016
International audience; 1. Eutrophication increases primary productivity and favours the predominance of floating vegetation in wetlands. Carbon (C) fluxes in wetlands are strongly driven by primary productivity and can differ by vegetation type. However, to the best of our knowledge, the role of eutrophication in C fluxes has rarely been assessed. 2. Consequently, we aimed to measure the seasonal variation in carbon dioxide (CO2) and methane (CH4) fluxes at six aquatic sites in four temperate wetlands, ranging along a gradient of sediment total phosphorus content, and determine whether C fluxes correlate with above-ground net primary productivity (ANPP) and plant community composition alon…