Search results for "Method development"

Are analysts doing method validation in liquid chromatography?

2014

International audience; Method validation is being applied in the reported analytical methods for decades. Even before this protocol was defined, authors already somehow validated their methods without full awareness. They wished to assure the quality of their work. Validation is an applied approach to verify that a method is suitable and rugged enough to function as a quality control tool in different locations and times. The performance parameters and statistical protocols followed throughout a validation study vary with the source of guidelines. Before single laboratory validation, an analytical method should be fully developed and optimized. The purpose of the validation is to confirm p…

Global retention models and their application to the prediction of chromatographic fingerprints

2020

Abstract The resolution of samples containing unknown compounds of different nature, or without standards available, as is the case of chromatographic fingerprints, is still a challenge. Possibly, the most problematic aspect that prevents systematic method development is finding models that describe without bias the retention behaviour of the compounds in the samples. In this work, the use of global models (able to describe the whole sample) is proposed as an alternative to the use of individual models for each solute. Global models contain parameters that are specific for each solute, while other parameters ‒related to the column and solvent‒ are common for all solutes. A special regressio…

Analytical study and exposure to Fusarium mycotoxins = Estudio analítico y de exposición a micotoxinas de Fusarium

2015

Las micotoxinas son metabolitos secundarios de determinadas especies fúngicas que se encuentran habitualmente en los alimentos. El Reglamento No. 1881/2006 de la Comisión Europea, parcialmente modificado por posteriores Reglamentos, establece los contenidos máximos de micotoxinas para diferentes productos alimenticios. La introducción de estas normas pone de manifiesto el interés por el conocimiento de valores relativos a la concentración de estos tóxicos para poder evaluar riesgos y adoptar las medidas necesarias para proteger la salud de los consumidores. Los productos agrícolas contaminados, especialmente cereales y sus derivados, son la principal fuente de micotoxinas en la dieta de la …

A new approach to sensitivity testing with 4-year olds

2015

In order to have valid results from sensory experiments with pre-schoolers, there is a need to match the sensory test to the abilities of children. A new approach for sensitivity testing with 4- year olds was therefore developed to accommodate their abilities as well as to engage them in the task. Inspired by the principles of authenticity tests, a protocol was designed to induce an affective response to a paired comparison test. This protocol included a story with magical characters, and the children were asked to identify if water samples with different concentrations of diluted basic tastes were “magic” water or regular water. Water was labelled as “magic” to trigger an emotional respons…

Two-dimensional liquid chromatography - high resolution mass spectrometry method for simultaneous monitoring of 70 regulated and emerging mycotoxins …

2020

Liquid Phase Micro-Extraction of Linear Alkylbenzene Sulfonate Anionic Surfactants in Aqueous Samples

2011

Hollow fiber liquid phase micro-extraction (LPME) of linear alkylbenzene sulfonates (LAS) from aqueous samples was studied. Ion pair extraction of C10, C11, C12 and C13 homologues was facilitated with trihexylamine as ion-pairing agent, using di-n-hexylether as solvent for the supported liquid membrane (SLM). Effects of extraction time, acceptor buffer concentration, stirring speed, sample volume, NaCl and humic acids were studied. At 10–50 µg L−1 linear R2-coefficients were 0.99 for C10 and C11 and 0.96 for C12. RSD was typically ~15%. Three observations were especially made. Firstly, LPME for these analytes was unusually slow with maximum enrichment observed after 15–24 h (depending on sa…

DEVELOPMENT & QUALIFICATION OF BIOASSAYS FOR THE DETERMINATION OF THE BIOACTIVITY, PREDICTIVE PHARMACOKINETICS AND POTENTIAL IMMUNOGENICITY OF THERAP…

2021

The critical quality attributes of a given biotherapeutic monoclonal antibody (mAb), its molecular characterization, functional assessment and effector function analysis should be defined and profiled in detail during the life cycle of a biotherapeutic drug. In the past, this product characterization was simple and standardized. In today’s complex world of biologics, success demands a more thoughtful approach and drug developers are investing in advanced analytics much earlier in the development process. Indeed, investing in selected sophisticated and state of the art analytics in early developmental phases of a therapeutic monoclonal antibody may mitigate risks by confirming that the drug …