Search results for "Method"

showing 10 items of 13253 documents

Drosophila Food-Associated Pheromones: Effect of Experience, Genotype and Antibiotics on Larval Behavior

2016

International audience; Animals ubiquitously use chemical signals to communicate many aspects of their social life. These chemical signals often consist of environmental cues mixed with species-specific signals-pheromones-emitted by conspecifics. During their life, insects can use pheromones to aggregate, disperse, choose a mate, or find the most suitable food source on which to lay eggs. Before pupariation, larvae of several Drosophila species migrate to food sources depending on their composition and the presence of pheromones. Some pheromones derive from microbiota gut activity and these food-associated cues can enhance larval attraction or repulsion. To explore the mechanisms underlying…

0301 basic medicinemelanogasterlcsh:Medicine[ SDV.BA ] Life Sciences [q-bio]/Animal biologyBiochemistryPheromonesLarvaeAntibioticsMedicine and Health Sciencesinsectslcsh:ScienceAnimal Signaling and CommunicationLarvaMultidisciplinaryInsect MetamorphosisbiologyAnimal BehaviorBehavior AnimalEcologyAntimicrobialscommunicationDrosophila Melanogaster[SDV.BA]Life Sciences [q-bio]/Animal biologyaggressionsex-pheromonesDrugsAnimal ModelsAttractionPupaSex pheromoneLarvacourtshipNeurosciences (Sciences cognitives)DrosophilaDrosophila melanogasterCuesrecognitionPupariationResearch ArticleattractionComputer and Information SciencesArthropodaGenotypeZoologyResearch and Analysis MethodsMicrobiology03 medical and health sciencesModel OrganismsInvertebrate ZoologySEX-PHEROMONES;MELANOGASTER;AGGRESSION;COURTSHIP;COMMUNICATION;RECOGNITION;ATTRACTION;EVOLUTION;MUTATION;INSECTSMicrobial ControlevolutionAnimalsDrosophilaSensory cuePharmacologyBehaviorMetamorphosisData Visualizationlcsh:RfungiOrganismsBiology and Life SciencesPupaebiology.organism_classificationZoologie des invertébrésInvertebratesColor Codes030104 developmental biologyFoodOdorantslcsh:QmutationZoologyEntomologyNeuroscienceDevelopmental Biology
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Efficient and reproducible experimental infections of rats with Blastocystis spp.

2018

Although Blastocystis spp. infect probably more than 1 billion people worldwide, their clinical significance is still controversial and their pathophysiology remains poorly understood. In this study, we describe a protocol for an efficient and reproducible model of chronic infection in rats, laying the groundwork for future work to evaluate the pathogenic potential of this parasite. In our experimental conditions, we were unable to infect rats using vacuolar forms of an axenically cultivated ST4 isolate, but we successfully established chronic infections of 4 week-old rats after oral administration of both ST3 and ST4 purified cysts isolated from human stool samples. The infection protocol …

0301 basic medicinemodèle animal[SDV]Life Sciences [q-bio]lcsh:MedicineBlastocystis Infections[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]souris[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunityFecesblastocyste[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]Medicine and Health SciencesParasite hostingCystratmodèle pour les maladies humaineslcsh:Scienceblastocyst stageProtozoansGastrointestinal tractMice Inbred BALB CMice Inbred C3HMultidisciplinarybiologyaxenic cultureEukaryotaPathophysiologyanimal models3. Good health[SDV] Life Sciences [q-bio]Separation ProcessesExperimental Organism SystemsAnatomyResearch ArticlemiceColonMouse ModelsResearch and Analysis MethodsMicrobiologyculture axeniqueMicrobiology03 medical and health sciencesModel OrganismsmedicineParasitic DiseasesAnimalsHumansClinical significanceAnimal Models of Disease[SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunityDistillationBlastocystisHost (biology)lcsh:ROrganismsBiology and Life Sciencesbiology.organism_classificationmedicine.disease[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyParasitic ProtozoansRatsMice Inbred C57BLGastrointestinal TractChronic infectionDisease Models AnimalAnimal Models of Infection030104 developmental biologyBlastocystisAnimal Studieslcsh:Q[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyParasitic Intestinal DiseasesDigestive System
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Association between ribs shape and pulmonary function in patients with Osteogenesis Imperfecta

2019

Graphical abstract

0301 basic medicinemusculoskeletal diseasesVital capacityPulmonary functionMedicinaKyphosisScoliosisPulmonary function testing03 medical and health sciencesFEV1/FVC ratio0302 clinical medicinemedicineRespiratory functionLung volumeslcsh:Science (General)ComputingMethodologies_COMPUTERGRAPHICSRib cagelcsh:R5-920Geometric morphometricsMultidisciplinarybusiness.industryAnatomymedicine.diseasemusculoskeletal system030104 developmental biologyScoliosis030220 oncology & carcinogenesisRib cageOriginal ArticleOsteogenesis imperfectaThoracic spinebusinesslcsh:Medicine (General)lcsh:Q1-390Journal of Advanced Research 21: 177-185 (2020)
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The F4/AS01B HIV-1 Vaccine Candidate Is Safe and Immunogenic, But Does Not Show Viral Efficacy in Antiretroviral Therapy-Naive, HIV-1-Infected Adults…

2016

Supplemental Digital Content is available in the text

0301 basic medicinemyalgiaAdultCD4-Positive T-LymphocytesMalemedicine.medical_specialty4850AdolescentMedizinHIV InfectionsVacunesPlaceboAntibodies Virallaw.invention03 medical and health sciencesYoung Adult0302 clinical medicineRandomized controlled triallawInternal medicineVIH (Virus)medicineHumansSingle-Blind Method030212 general & internal medicineYoung adultAdverse effectAIDS VaccinesVaccinesHIV (Viruses)business.industryClinical Trial/Experimental StudyGeneral MedicineConfidence intervalCD4 Lymphocyte CountClinical trial030104 developmental biologyAnti-Retroviral AgentsImmunologyAntibody FormationComputingMethodologies_DOCUMENTANDTEXTPROCESSINGHIV-1Femalemedicine.symptombusinessViral loadResearch Article
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Ferritin and C-reactive protein are predictive biomarkers of mortality and macrophage activation syndrome in adult onset Still's disease. Analysis of…

2020

ObjectiveTo assess the predictive role of ferritin and C-reactive protein (CRP) on occurrence of macrophage activation syndrome (MAS) and mortality in patients with adult onset Still's disease (AOSD), a rare and severe disease, included in the multicentre Gruppo Italiano di Ricerca in Reumatologia Clinica e Sperimentale (GIRRCS) cohort.MethodsThe predictive role, at the time of diagnosis, of serum levels of ferritin and CRP on occurrence of MAS and mortality, was evaluated by logistic regression analyses and receiver-operating characteristic (ROC) curves were built to identify patients at high risk of MAS and mortality, respectively.ResultsIn assessed 147 patients with AOSD, levels of ferri…

0301 basic medicinemyalgiaAdult-OnsetMalePredictive Value of TestPathology and Laboratory MedicineLogistic regressionBiochemistryGastroenterologyCohort StudiesMathematical and Statistical Techniques0302 clinical medicineMedicine and Health SciencesMultidisciplinarybiologyMacrophage Activation SyndromeStatisticsQRMiddle AgedC-Reactive ProteinsC-Reactive ProteinPredictive value of testsPhysical SciencesCohortRegression AnalysisMedicineFemalemedicine.symptomStill's Disease Adult-OnsetResearch ArticleCohort studyHumanAdultmedicine.medical_specialtyPatientsLogistic ModelInflammatory DiseasesSciencePainStill's Disease Adult-Onset.Research and Analysis Methods03 medical and health sciencesAdult; Biomarkers; C-Reactive Protein; Cohort Studies; Female; Ferritins; Humans; Logistic Models; Macrophage Activation Syndrome; Male; Middle Aged; Predictive Value of Tests; ROC Curve; Still's Disease Adult-OnsetSigns and SymptomsDiagnostic MedicinePredictive Value of TestsInternal medicinemedicineHumansStatistical Methods030203 arthritis & rheumatologyFerritinbusiness.industryC-reactive proteinBiology and Life SciencesProteinsProtein ComplexesMyalgiaBiomarkermedicine.diseaseStill's DiseaseHealth CareFerritin030104 developmental biologyLogistic ModelsROC CurveMacrophage activation syndromeMultivariate AnalysisFerritinsbiology.proteinCohort StudiebusinessMathematicsBiomarkers
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Current concepts in the prevention of pathogen transmission via blood/plasma-derived products for bleeding disorders

2015

The pathogen safety of blood/plasma-derived products has historically been a subject of significant concern to the medical community, Measures such as donor selection and blood screening have contributed to increase the safety of these products, but pathogen transmission does still occur. Reasons for this include lack of sensitivity/specificity of current screening methods, lack of reliable screening tests for some pathogens (e.g. prions) and the fact that many potentially harmful infectious agents are not routinely screened for. Methods for the purification/inactivation of blood/plasma-derived products have been developed in order to further reduce the residual risk, but low concentrations…

0301 basic medicineriesgohumanosUltrafiltrationBacteremiaBlood DonorsHepacivirus030204 cardiovascular system & hematologyParasitemia/dk/atira/pure/subjectarea/asjc/2700/27200302 clinical medicineBlood plasmaScreening methodMedicinePathogenChromatographyultrafiltraciónfungemiaTransmission (medicine)Blood ScreeningbacteriemiaHematologyBlood Coagulation DisordersChromatography Ion ExchangeSettore MED/07 - Microbiologia e Microbiologia Clinicatransfusión de componentes sanguíneos/dk/atira/pure/subjectarea/asjc/2700/2730trastornos de la coagulación sanguíneaOncologyVIH-1RiskHepatitis B virusHaemophiliaBlood Component TransfusionHaemophiliaArticlepatógenos transmitidos por la sangre03 medical and health sciencesBlood-Borne PathogensHumansViremiacromatografíaBlood safety; Clotting; Haemophilia; Pathogen; TransfusionPathogenbusiness.industryDonor selectionTransfusionClottingdonantes de sangrevirus de la hepatitis Bmedicine.diseaseResidual risk030104 developmental biologyImmunologyHIV-1businessBlood safetyBlood Reviews
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Effects of Infant Formula With Human Milk Oligosaccharides on Growth and Morbidity: A Randomized Multicenter Trial

2017

Objectives:The aim of the study was to evaluate the effects of infant formula supplemented with 2 human milk oligosaccharides (HMOs) on infant growth, tolerance, and morbidity. Methods:Healthy infants, 0 to 14 days old, were randomized to an intact-protein, cow's milk-based infant formula (control, n=87) or the same formula with 1.0g/L 2fucosyllactose (2FL) and 0.5g/L lacto-N-neotetraose (LNnT) (test, n=88) from enrollment to 6 months; all infants received standard follow-up formula without HMOs from 6 to 12 months. Primary endpoint was weight gain through 4 months. Secondary endpoints included additional anthropometric measures, gastrointestinal tolerance, behavioral patterns, and morbidit…

0301 basic medicinesafetyMalePediatricsmedicine.medical_specialtyMEDLINEOligosaccharidesWeight Gainlaw.inventionbronchitis03 medical and health scienceschemistry.chemical_compound2'-FucosyllactoseRandomized controlled trialDouble-Blind Method2fucosyllactose; bronchitis; lacto-N-neotetraose; safety; tolerancelawMulticenter trialmedicineAnimalsHumansLacto-N-neotetraoseRespiratory Tract Infectionslacto-N-neotetraose2′fucosyllactose030109 nutrition & dieteticstoleranceMilk Humanbusiness.industryGastroenterologyOriginal Articles: NutritionInfant Newbornfood and beveragesInfantProtective Factorsmedicine.diseaseInfant Formula030104 developmental biologyMilkchemistryInfant formulaPediatrics Perinatology and Child Health2'fucosyllactose bronchitis lacto-N-neotetraose safety toleranceBronchitisFemalemedicine.symptombusinessWeight gainFollow-Up Studies
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Bacteria classification using minimal absent words

2017

Bacteria classification has been deeply investigated with different tools for many purposes, such as early diagnosis, metagenomics, phylogenetics. Classification methods based on ribosomal DNA sequences are considered a reference in this area. We present a new classificatier for bacteria species based on a dissimilarity measure of purely combinatorial nature. This measure is based on the notion of Minimal Absent Words, a combinatorial definition that recently found applications in bioinformatics. We can therefore incorporate this measure into a probabilistic neural network in order to classify bacteria species. Our approach is motivated by the fact that there is a vast literature on the com…

0301 basic medicinesupervised classificationRelation (database)Computer science0102 computer and information sciences01 natural sciencesMeasure (mathematics)03 medical and health sciencesProbabilistic neural networkcombinatorics on wordsprobabilistic neural networkminimal absent wordlcsh:R5-920Settore INF/01 - Informaticabusiness.industryBacterial taxonomyPattern recognitionbacteria classificationGeneral MedicineCombinatorics on words030104 developmental biology010201 computation theory & mathematicsMetagenomicsClassification methodsArtificial intelligencebusinesslcsh:Medicine (General)AIMS Medical Science
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AB0691 INCREASED CARDIOVASCULAR RISK IN MIXED CONNECTIVE TISSUE DISEASE: EVALUATION OF MACROVASCULAR INVOLVEMENT AND ITS PREDICTORS BY AORTIC PULSE W…

2019

Background: Macrovascular involvement and cardiovascular (CV) risk have not been sufficiently studied in patients with mixed connective tissue disease (MCTD). In particular, the gold standard assessment method of aortic stiffness carotid-femoral pulse wave velocity (cfPWV) (1) has never been evaluated in patients with this disease. Objectives: Aims of the present study were to examine cfPWV in MCTD and to evaluate its associations with MCTD associated parameters and traditional CV risk factors. Methods: cfPWV measurements were performed in 43 MCTD patients and 107 healthy controls. The difference between cfPWV in the two groups was statistically examined and subsequently controlled for the …

030203 arthritis & rheumatology0301 basic medicinemedicine.medical_specialtybusiness.industryGold standardConfoundingmedicine.disease03 medical and health sciences030104 developmental biology0302 clinical medicineMixed connective tissue diseaseBlood pressureInternal medicineAssessment methodsmedicineCardiologyAortic stiffnessIn patientbusinessPulse wave velocityAbstracts accepted for Publication
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Influence of the metaphysis positioning in a new reverse shoulder prosthesis

2016

Aim of this work is to investigate the behaviour of a new reverse shoulder prosthesis, characterized by a humeral metaphysis with a variable offset, designed to increase the range of movements and to reduce the impingement. In particular, by means of virtual prototypes of the prosthesis, different offset values of the humeral metaphysis have been analysed in order to find the best positioning able to maximize the range of movements of the shoulder joint. The abduction force of the deltoid, at different offset values, has been also estimated. The study has been organized as follows. In the first step, the point clouds of the surfaces of the different components of the prosthesis have been ac…

030222 orthopedicsScannerOffset (computer science)business.industryComputer scienceDeltoid curvePoint cloud030229 sport sciencesMetaphysisMoiré patternFinite element method03 medical and health sciences0302 clinical medicinemedicine.anatomical_structuremedicineComputer visionShoulder jointArtificial intelligencebusinessReverse Engineering CAD Reverse Shoulder Prosthesis Range of Movements
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