Search results for "Methylene"

showing 10 items of 821 documents

A Hazelnut-Enriched Diet Modulates Oxidative Stress and Inflammation Gene Expression without Weight Gain

2019

Introduction. Inflammation is associated with obesity condition and plays a pivotal role in the onset and progression of many chronic diseases. Among several nutraceutical foods, hazelnuts (Corylus avellana L.) are considered an excellent anti-inflammatory and hypolipidemic food being the second richest source of monounsaturated fatty acids among nuts and because they are rich in vitamins, minerals, and phenolic compounds. Materials and Methods. A prospective pilot clinical trial on 24 healthy volunteers who consumed daily, as a snack, 40 g of hazelnuts (261.99 kcal/1096.17 kJ) for six weeks was conducted. Anthropometric measurements, body composition analysis, and nutrigenomic analysis on …

0301 basic medicineAgingAntioxidantArticle Subjectmedicine.medical_treatmentGene ExpressionInflammationPilot Projects030204 cardiovascular system & hematologyBiologymedicine.disease_causeSettore BIO/09BiochemistryCalcitriol receptorCorylus; Gene Expression; Healthy Volunteers; Humans; Inflammation; Middle Aged; Obesity; Oxidative Stress; Pilot Projects; Prospective Studies03 medical and health sciences0302 clinical medicineNutraceuticalCorylusmedicineHumansFood scienceObesityProspective Studieslcsh:QH573-671Inflammationlcsh:CytologySettore BIO/12Cell BiologyGeneral MedicineMiddle Agedmedicine.diseaseObesityHealthy VolunteersOxidative Stress030104 developmental biologyMethylenetetrahydrofolate reductasebiology.proteinClinical Studymedicine.symptomWeight gainOxidative stress
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Role of nitric oxide pathway in the conditioned rewarding effects of MDMA in mice.

2017

It is estimated that 2.1 million young adults used MDMA/Ecstasy in the last year in Europe. Vulnerable subjects can develop dependence after MDMA abuse but currently there does not exist an effective treatment for this disorder. The nitric oxide (NO) pathway seems to have an important role on the rewarding effects of different drugs and has been proposed as a new pharmacological treatment for psychostimulant addiction. In the present study, we intend to evaluate whether the blockade of the NO synthesis (NOS) interferes with the rewarding effects of MDMA in the conditioned preference place (CPP) paradigm in young adult male mice. Our results indicated that mice treated with 7-nitroindazole (…

0301 basic medicineHallucinogenMaleMDMA7-NitroindazoleIndazolesmedia_common.quotation_subjectN-Methyl-34-methylenedioxyamphetamineEcstasyConditioning ClassicalEnsayos clínicosPharmacologyNitric OxideNitric oxide03 medical and health sciencesBehavioral Neurosciencechemistry.chemical_compoundMice0302 clinical medicineRewardmental disordersConditioning PsychologicalmedicineAnimalsDrogasmedia_commonbiologyAddictionMDMABlockadeNitric oxide synthaseEfectos fisiológicos030104 developmental biologychemistrybiology.proteinHallucinogensConditioning OperantCentral Nervous System StimulantsNitric Oxide SynthasePsychologyEstupefacientepsychological phenomena and processes030217 neurology & neurosurgerymedicine.drugBehavioural brain research
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Anticancer potential of novel α,β-unsaturated γ-lactam derivatives targeting the PI3K/AKT signaling pathway.

2021

Abstract Six recently synthesized alkyl (Z)-2-(2-oxopyrrolidin-3-ylidene)acetates were evaluated for their potential as cytotoxic and anticancer agents. All compounds were tested in the ERα positive MCF-7, triple negative MDA-MB-231, and Her2+ SKBR-3 breast cancer cell lines. The most lipophilic derivatives, bearing the 4-isopropylphenyl (2) or 4-tert-butylphenyl (3) group at the γ-lactam nitrogen, proved to be cytotoxic against all the cancer cell lines tested (IC50 values ranging from 18 to 63 μM), exerting their greatest activity in SKBR-3 cells, with IC50 values of 33 and 18 μM, respectively. Biological studies showed that the cytotoxic effects of 2 and 3 are accompanied by apoptotic de…

0301 basic medicineLactamsCell SurvivalAntineoplastic AgentsApoptosisBreast NeoplasmsBiochemistry03 medical and health scienceschemistry.chemical_compoundPI3K/AKT signaling pathwayPhosphatidylinositol 3-Kinases0302 clinical medicinebreast cancerCytotoxic T cellHumansα-methylene-γ-lactamsαβ-unsaturated lactamsCytotoxicityPI3K/AKT/mTOR pathwayPhosphoinositide-3 Kinase InhibitorsPharmacologychemistry.chemical_classificationBiological ProductsMolecular StructureChemistryCell growthAkt/PKB signaling pathwayCell CycleEpithelial Cells030104 developmental biologyEnzymeGene Expression RegulationApoptosis030220 oncology & carcinogenesisCancer researchLactamcytotoxicityFemaleProto-Oncogene Proteins c-aktBiochemical pharmacology
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Anti-Inflammatory Activity and Cheminformatics Analysis of New Poten t 2-Substituted 1-Methyl-5-Nitroindazolinones.

2018

After the identification of the anti-inflammatory properties of VA5-13l (2-benzyl-1- methyl-5-nitroindazolinone) in previous investigations, some of its analogous compounds were designed, synthesized and evaluated in two anti-inflammatory methods: LPS-enhanced leukocyte migration assay in zebrafish; and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. The products evaluated (3, 6, 8, 9 and 10) showed the lower values of relative leukocyte migration at 30#181;M (0.14, 0.07, 0.10, 0.13 and 0.07, respectively), while in ear edema and myeloperoxidase activity methods, all the compounds reduced inflammation, only 4 and 16 yielded unsatisfactory results. The relationship linkin…

0301 basic medicineLipopolysaccharidesLeukocyte migrationIndazolesInformaticsStereochemistrymedicine.drug_classSubstituentNitric Oxide Synthase Type IINitric OxideAnti-inflammatory03 medical and health scienceschemistry.chemical_compoundStructure-Activity RelationshipDrug DiscoverymedicineMoietyStructure–activity relationshipAnimalsHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyMethyleneComputingMilieux_MISCELLANEOUSAlkylZebrafishchemistry.chemical_classificationIndazoleCyclooxygenase 2 InhibitorsDose-Response Relationship DrugMolecular StructureChemistryTumor Necrosis Factor-alphaAnti-Inflammatory Agents Non-SteroidalGeneral MedicineNitro Compounds3. Good health030104 developmental biologyCyclooxygenase 2Current topics in medicinal chemistry
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Blockade of nitric oxide signalling promotes resilience to the effects of social defeat stress on the conditioned rewarding properties of MDMA in mice

2020

Abstract MDMA abuse continues being a serious problem in our society. Environmental factors, such as stress, increase the vulnerability of individuals to develop drug abuse and we have observed that exposure to social defeat (SD) stress alters the sensitivity of mice to the rewarding effects of MDMA in the conditioned place preference (CPP) paradigm. In the present study, we evaluated the role of the nitric oxide (NO) pathway in the effects of SD on the rewarding properties of MDMA. Three groups of mice were treated with an inhibitor of NO synthesis, 7-nitroindazole (0, 7.25 and 12.5 mg/kg), before each exposure to SD and place conditioning with MDMA (1.25 mg/kg) on PND 54, 56, 58, and 60. …

0301 basic medicineMaleCancer Researchmedicine.medical_specialty7-NitroindazoleIndazolesMDMAPhysiologyN-Methyl-34-methylenedioxyamphetamineClinical BiochemistryHippocampusMice Inbred StrainsStriatum030204 cardiovascular system & hematologyNitric OxideBiochemistrySocial defeat03 medical and health scienceschemistry.chemical_compoundMice0302 clinical medicineSocial defeatInternal medicineConditioning Psychologicalmental disordersmedicineAnimalsPrefrontal cortex7-NitroindazoleSocial stressDose-Response Relationship Drugbusiness.industryMDMANitric oxideConditioned place preferenceConditioned place preference030104 developmental biologyEndocrinologychemistrybusinessStress Psychologicalpsychological phenomena and processesmedicine.drugSignal Transduction
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Stem cells distribution, cellular proliferation and migration in the adult Austrolebias charrua brain.

2017

Our previous studies demonstrated that Austrolebias charrua annual fish is an excellent model to study adult brain cell proliferation and neurogenesis due to the presence of active and fast neurogenesis in several regions during its short lifespan. Our main goal was to identify and localize the cells that compose the neurogenic areas throughout the Austrolebias brain. To do this, we used two thymidine halogenated analogs to detect cell proliferation at different survival times: 5-chloro-2'-deoxyuridine (CldU) at 1day and 5-iodo-2'-deoxyuridine (IdU) at 30days. Three types of proliferating cells were identified: I - transient amplifying or fast cycling cells that uptake CldU; II - stem cells…

0301 basic medicineMalePopulationVimentinCell Count03 medical and health sciencesCyprinodontiformes0302 clinical medicineImaging Three-DimensionalCell MovementAnimalsStem Cell NicheeducationColoring AgentsMolecular BiologyCell Proliferationeducation.field_of_studybiologyCell growthGeneral NeuroscienceStem CellsNeurogenesisBrainAnatomyNestinbiology.organism_classificationImmunohistochemistryCell biologyMethylene Blue030104 developmental biologybiology.proteinNeurology (clinical)NeuNStem cell030217 neurology & neurosurgeryAustrolebiasDevelopmental BiologyBrain research
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Homocysteine concentration in coronary artery disease: Influence of three common single nucleotide polymorphisms.

2017

Whether single nucleotide polymorphisms (SNPs) of homocysteine metabolism enzymes influence the rate of cardiovascular (CV) events in coronary artery disease (CAD) patients remains controversial.In this analysis, 1126 subjects from the AtheroGene study with CAD and 332 control subjects without known CAD were included. The following SNPs were investigated: methylentetrahydrofolate reductase (MTHFR-C667T), methionin synthetase (MS-D919G), and cystathionin beta synthetase (CBS-I278T). The endpoint was the combination of cardiovascular death, stroke, and non-fatal myocardial infarction (N = 286). The median follow-up time was 6.4 years. Kaplan-Meier curve analysis showed an increasing event rat…

0301 basic medicineMaleTime FactorsHomocysteineEndocrinology Diabetes and MetabolismMyocardial InfarctionMedicine (miscellaneous)Coronary Artery DiseaseKaplan-Meier Estimate030204 cardiovascular system & hematologyReductaseGastroenterology5-Methyltetrahydrofolate-Homocysteine S-MethyltransferaseCoronary artery diseasechemistry.chemical_compound0302 clinical medicineGene FrequencyRisk FactorsMyocardial infarctionStrokeHomocysteineGeneticsNutrition and DieteticsbiologyHomozygoteMiddle AgedStrokePhenotypeArea Under CurveDisease ProgressionFemaleCardiology and Cardiovascular Medicinemedicine.medical_specialtyHeterozygoteCystathionine beta-SynthaseSingle-nucleotide polymorphismPolymorphism Single NucleotideRisk Assessment03 medical and health sciencesPredictive Value of TestsInternal medicinemedicineHumansGenetic Predisposition to DiseaseGenetic Association StudiesMethylenetetrahydrofolate Reductase (NADPH2)AgedProportional Hazards ModelsChi-Square DistributionCurve analysismedicine.disease030104 developmental biologychemistryROC CurveMethylenetetrahydrofolate reductaseCase-Control Studiesbiology.proteinBiomarkersNutrition, metabolism, and cardiovascular diseases : NMCD
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PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and Prothrombin 20210A in Splanchnic Vein Thrombosis: Analysis of Individual Patient Data From Three Prospec…

2015

There are no univocal opinions on the role of genetic thrombophilia on splanchnic vein thrombosis (SVT). We defined genetic thrombophilia the presence of one of these thrombophilic genetic factors (THRGFs): PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and prothrombin 20210A.To evaluate the frequencies of these THRGFs in SVT patients, we analyzed individual data of 482 Caucasian patients, recruited from 2000 to 2014 in three prospective studies. SVT was defined as the presence of thrombosis of portal (PVT), mesenteric (MVT), splenic (SPVT), cava (CT), and hepatic vein (Budd Chiari syndrome, BCS). Pre-hepatic SVT (pre-HSVT) was defined as PVT with or without MVT/SPVT, without BCS. Post-hepatic SV…

0301 basic medicinemedicine.medical_specialtyHepatologybiologybusiness.industry030204 cardiovascular system & hematologymedicine.diseaseThrombophiliaGastroenterologyThrombosisSurgeryPortal vein thrombosis03 medical and health sciences030104 developmental biology0302 clinical medicineSplanchnic vein thrombosisInternal medicineMethylenetetrahydrofolate reductaseBudd–Chiari syndromebiology.proteinMedicineOriginal ArticlebusinessProspective cohort studyMyeloproliferative neoplasm
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Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism?

2019

Background: Homocysteine is a sulfur amino acid whose metabolism is activated in two pathways: remethylation to methionine, which requires folate and vitamin B12, and transsulfuration to cystathionine, which needs pyridoxal-5’-phosphate. High homocysteine level increases the risk of developing heart disease, stroke, peripheral vascular diseases, and cognitive impairment. Some evidence showed that exposure to these metals increased plasma homocysteine levels. Methods: A systematic review was carried out to clarify the relationship between homocysteine blood levels and exposure to toxic heavy metals (Lead, Cadmium, Mercury, and Chromium). Results: The results of this systematic review i…

0301 basic medicinemedicine.medical_specialtyHyperhomocysteinemiamercury6HomocysteinePhysiologycadmiumvitamin b<sub>6</sub>Clinical BiochemistryCadmium; Chromium; Folate; Lead; Mercury; Methionine; MTHFR; Vitamin B; 12; Vitamin B; 6TranssulfurationReview010501 environmental sciencesfolate01 natural sciencesBiochemistryvitamin B603 medical and health scienceschemistry.chemical_compoundInternal medicinemedicineVitamin B12Vitamin BMolecular Biology0105 earth and related environmental sciencesmethionineleadMethioninebiologybusiness.industrylcsh:RM1-950Cell BiologyMetabolismvitamin B12medicine.diseaseCystathionine beta synthase12lcsh:Therapeutics. Pharmacology030104 developmental biologyEndocrinologychemistryMethylenetetrahydrofolate reductaseMTHFRbiology.proteinchromiumbusinessvitamin b<sub>12</sub>Antioxidants
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CCDC 2211279: Experimental Crystal Structure Determination

2023

Related Article: Anssi Peuronen, Anni Taponen, Elina Kalenius, Ari Lehtonen, Manu Lahtinen|2023|Angew.Chem.,Int.Ed.|62|e202215689|doi:10.1002/anie.202215689

11'1''-[(246-trimethylbenzene-135-triyl)tris(methylene)]tris(14-diazabicyclo[2.2.2]octan-1-ium) iodide bis(bis(trifluoromethanesulfonyl)azanide) acetonitrile solvateSpace GroupCrystallographyCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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