Search results for "Methylococcaceae"

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Draft genome of a novel methanotrophic Methylobacter sp. from the volcanic soils of Pantelleria Island

2021

AbstractThe genus Methylobacter is considered an important and often dominant group of aerobic methane-oxidizing bacteria in many oxic ecosystems, where members of this genus contribute to the reduction of CH4 emissions. Metagenomic studies of the upper oxic layers of geothermal soils of the Favara Grande, Pantelleria, Italy, revealed the presence of various methane-oxidizing bacteria, and resulted in a near complete metagenome assembled genome (MAG) of an aerobic methanotroph, which was classified as a Methylobacter species. In this study, the Methylobacter sp. B2 MAG was used to investigate its metabolic potential and phylogenetic affiliation. The MAG has a size of 4,086,539 bp, consists …

DNA BacterialMethanotrophMethane monooxygenaseSettore BIO/19 - Microbiologia GeneraleMicrobiologyVolcanic soilSoil03 medical and health scienceschemistry.chemical_compoundRNA Ribosomal 16SBotanyMolecular BiologyEcosystemPhylogenyFormaldehyde dehydrogenase030304 developmental biologyOriginal Paper0303 health sciencesbiologyMethanol dehydrogenase030306 microbiologyChemistryCarbon fixationTetrahydromethanopterinGeneral Medicinebiology.organism_classificationMethanotrophMetabolic potentialMetagenomicsEcological MicrobiologyMethylococcaceaebiology.proteinMethaneBacteriaAntonie van Leeuwenhoek
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Isolation of methanotrophic bacteria from termite gut.

2015

The guts of termites feature suitable conditions for methane oxidizing bacteria (MOB) with their permanent production of CH4 and constant supply of O2 via tracheae. In this study, we have isolated MOB from the gut contents of the termites Incisitermes marginipennis, Mastotermes darwiniensis, and Neotermes castaneus for the first time. The existence of MOB was indicated by detecting pmoA, the gene for the particulate methane monooxygenase, in the DNA of gut contents. Fluorescence in situ hybridization and quantitative real-time polymerase chain reaction supported those findings. The MOB cell titer was determined to be 10(2)-10(3) per gut. Analyses of the 16S rDNA from isolates indicated clos…

DNA Bacterialfood.ingredientMethane monooxygenaseColony Count MicrobialIsopteraReal-Time Polymerase Chain ReactionMicrobiologyMethylococcaceaeDNA RibosomalMicrobiologyfoodMastotermes darwiniensisRNA Ribosomal 16SAnimalsIn Situ Hybridization FluorescenceMethylocystis bryophilabiologyBacteriaSequence Analysis DNAbiology.organism_classificationGastrointestinal TractMicroscopy FluorescenceMethylocystaceaeMethylocystisMethylococcaceaebiology.proteinOxygenasesMethylocystis parvusMethaneMethylocystaceaeBacteriaMicrobiological research
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