Search results for "Mg2+"

showing 3 items of 3 documents

Proton Leakage Is Sensed by IM30 and Activates IM30-Triggered Membrane Fusion

2020

The inner membrane-associated protein of 30 kDa (IM30) is crucial for the development and maintenance of the thylakoid membrane system in chloroplasts and cyanobacteria. While its exact physiological function still is under debate, it has recently been suggested that IM30 has (at least) a dual function, and the protein is involved in stabilization of the thylakoid membrane as well as in Mg2+-dependent membrane fusion. IM30 binds to negatively charged membrane lipids, preferentially at stressed membrane regions where protons potentially leak out from the thylakoid lumen into the chloroplast stroma or the cyanobacterial cytoplasm, respectively. Here we show in vitro that IM30 membrane binding…

0301 basic medicineChloroplastsMembrane lipidsmembrane fusionMg2+CyanobacteriaThylakoidsCatalysisArticleVipp1Inorganic Chemistrylcsh:Chemistry03 medical and health sciencesMembrane Lipidsquartz crystal microbalanceProtein structureBacterial ProteinsPhysical and Theoretical ChemistryMg<sup>2+</sup>membrane bindingMolecular Biologylcsh:QH301-705.5SpectroscopyMembranes030102 biochemistry & molecular biologyChemistrypHOrganic ChemistrySynechocystisCD spectroscopyLipid bilayer fusionMembrane Proteinsfood and beveragesGeneral Medicinethylakoid membraneComputer Science ApplicationsChloroplastChloroplast stroma030104 developmental biologyMembranelcsh:Biology (General)lcsh:QD1-999CytoplasmThylakoidBiophysicsProtonsIM30Protein BindingInternational Journal of Molecular Sciences
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Mg2+ binding triggers rearrangement of the IM30 ring structure, resulting in augmented exposure of hydrophobic surfaces competent for membrane binding

2018

The "inner membrane-associated protein of 30 kDa" (IM30), also known as "vesicle-inducing protein in plastids 1" (Vipp1), is found in the majority of photosynthetic organisms that use oxygen as an energy source, and its occurrence appears to be coupled to the existence of thylakoid membranes in cyanobacteria and chloroplasts. IM30 is most likely involved in thylakoid membrane biogenesis and/or maintenance, and has recently been shown to function as a membrane fusion protein in presence of Mg2+ However, the precise role of Mg2+ in this process and its impact on the structure and function of IM30 remains unknown. Here, we show that Mg2+ binds directly to IM30 with a binding affinity of ∼1 mm …

0301 basic medicineMembrane fusion proteinChemistryPspALipid bilayer fusionIsothermal titration calorimetryMg2+Cell BiologyBiochemistry[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry03 medical and health sciences030104 developmental biologyThylakoidMembrane biogenesisBiophysicsFourier transform IREnergy sourceMolecular BiologyMembrane biophysicsIM30BiogenesisJournal of Biological Chemistry
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Effect of Mg 2+ ions co-doping on luminescence and defects formation processes in Gd 3 (Ga,Al) 5 O 12 :Ce single crystals

2017

The work was supported by the Institutional Research Funding IUT02-26 of the Estonian Ministry of Education and Research and the project 16-15569S of the Czech Science Foundation.

LuminescencePhotoluminescenceMaterials scienceAnalytical chemistryMg2+02 engineering and technologyCrystal structure01 natural sciencesInorganic ChemistryCe3+0103 physical sciences:NATURAL SCIENCES:Physics [Research Subject Categories]Electrical and Electronic EngineeringPhysical and Theoretical ChemistrySpectroscopyMulticomponent garnets010302 applied physicsOrganic ChemistryDopingRadioluminescenceAtmospheric temperature range021001 nanoscience & nanotechnologyAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsPhotoexcitationCrystallographyAbsorption bandScintillatorsSingle crystals0210 nano-technologyLuminescenceOptical Materials
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