Search results for "Micro"
showing 10 items of 23412 documents
THE ‘CRUCIFIED AND DEPOSED CHRIST’ OF MUSEO DIOCESANO OF PALERMO, ITALY. SCIENTIFIC STUDIES OF A SICILIAN PAPIER-MȂSCHÉ POLYCHROME SCULPTURE
2022
In this paper we describe scientific investigations on the artwork ‘Crucified and deposed Christ’ from the Diocesan Museum of Palermo. Multidisciplinary studies were carried out to obtain interesting information about constitutive materials and executive techniques of XVIII century Sicilian papier mâsché, as well as about the sculpture state of conservation and the recognition of the previous treatments.Examination of the polychrome sculpture was both non-invasive (IRR, IRC, UV fluorescence, EDXRF spectroscopy X-Radiography) and micro invasive (optical microscopy, scanning electron microscopy with energy dispersive X-ray spectroscopy SEM-EDX).SEM-EDX results showed the use of materials like…
Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella
2017
The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex effector repertoires. However, the limited availability of genome sequences from one of those lineages as well as other, remote branches of Bartonella has so far hampered comprehensive understanding of how the VirB/D4 T4SS and its effectors called Beps have shaped Bartonella evolution. Here, we repor…
Experimental evolution of an RNA virus in cells with innate immunity defects
2015
Experimental evolution studies have shown that RNA viruses respond rapidly to directional selection and thus can adapt efficiently to changes in host cell tropism, antiviral drugs, or other imposed selective pressures. However, the evolution of RNA viruses under relaxed selection has been less extensively explored. Here, we evolved vesicular stomatitis virus in mouse embryonic fibroblasts knocked-out for PKR, a protein with a central role in antiviral innate immunity. Vesicular stomatitis virus adapted to PKR-negative mouse embryonic fibroblasts in a gene-specific manner, since the evolved viruses exhibited little or no fitness improvement in PKR-positive cells. Full-length sequencing revea…
Evidence for Positive Selection in the Capsid Protein-Coding Region of the Foot-and-Mouth Disease Virus (FMDV) Subjected to Experimental Passage Regi…
2001
We present sequence data from two genomic regions of foot-and-mouth disease virus (FMDV) subjected to several experimental passage regimens. Maximum-likelihood estimates of the nonsynonymous-to-synonymous rate ratio parameter (dN/dS) suggested the action of positive selection on some antigenic sites of the FMDV capsid during some experimental passages. These antigenic sites showed an accumulation of convergent amino acid replacements during massive serial cytolytic passages and also in persistent infections of FMDV in cell culture. This accumulation was most significant at the antigenic site A (the G-H loop of capsid VP1), which includes an Arg-Gly-Asp (RGD) cellular recognition motif. Our …
Spore-forming parasites infecting muscles of freshwater fishes : ecology and epidemiology
2017
Fish parasites may potentially harm fisheries and aquaculture. Infected fish are unusable, even though they would not pose a direct risk to human health. The aim of this thesis was to investigate the ecology and epidemiology of three previously unknown or poorly known spore-forming parasites infecting muscles of economically important fish species. The first novel microsporidian species, here described as Microsporidium luciopercae, was found from pike-perch (Sander lucioperca) and European perch (Perca fluviatilis) and formed opaque-looking patches in the muscles. The second novel microsporidian species, Myosporidium spraguei, was found from pike-perch and burbot (Lota lota) and occurred w…
A parasitoloxia e a doutrina do contáxio animado: a helmintiase e a sarna na España do século XIX
1990
Les explicacions parasitològiques del contagi eren tant teòriques com observacionals. La triquinosi, la sarna i la helmintiasi van ser exemples primerencs que van demostrar un contagi viu. A principis del segle XIX, en la medicina espanyola, igual que en la medicina europea, es rebutjava el contagi animat excepte en el cas d'alguns paràsits animals com la sarna. En uns altres, com en la helmintiasi intestinal, s'acceptava el seu origen viu, però no la seua contagiositat, i es defensava la seua generació espontània fins a ben entrat el segle XIX. En el “Diccionari de Ciències Mèdiques”, l'estudi dels cucs és quasi exclusivament morfològic i es defensa com a causa de la seua presència en l'ho…
Implementation of participatory sensing approach in mobile vehicle based sensor networks
2013
In this paper author describes his research with the goal to develop and experimentally verify specific data recording and processing methodologies based on participatory sensing approach implementation in mobile vehicle based sensor networks. To reach this goal, author performed study of literature, testing of hypothesis using general purpose computer devices, adaptation of smartphones for participatory sensing, development of special purpose embedded devices, practical experiments with selected technical equipment and software as well as gathering of experimental results and following statistical analysis. The result of this research are several data acquisition and processing methodologi…
Morphology and composition of particulate matter in the air of urban area
2019
Zanieczyszczenie powietrza przez pyl zawieszony (PM) staje się w ostatnich latach coraz bardziej znaczące. Stwierdzono, że unoszące się w powietrzu cząsteczki niosą różne niekorzystne skutki dla środowiska i zdrowia ludzkiego. Aby poszerzyć wiedzę na temat takich zanieczyszczeń, pożądana jest szczegółowa charakterystyka rozmiarów, morfologii i składu chemicznego poszczególnych cząstek zawieszonych w po- wietrzu. W prezentowanych badaniach mikrocząsteczki powietrza zostały pobrane z filtrów taśmowych z włókna szklanego, które są powszechnie stosowane w analizatorach cząstek stałych MP101M. Próbki zostały zebrane w Kutnej Horze (Republika Czeska), były badane metodami skaningowej mikroskopii …
Impact of Ageing on Pea Protein Volatile Compounds and Correlation with Odor
2022
Vegetal proteins are of high interest for their many positive aspects, but their ‘beany’ off-flavor is still limiting the consumer’s acceptance. The aim of this work was to investigate the conservation of pea protein isolate (PPI) during time and especially the evolution of their organoleptic quality under two storage conditions. The evolution of the volatile compounds, the odor and the color of a PPI has been investigated during one year of storage. PPI was exposed to two treatments mimicking a lack of control of storage conditions: treatment A with light exposition at ambient temperature (A—Light 20 °C) and treatment B in the dark but with a higher temperature …
Rational Herding in Reward-Based Crowdfunding: An MTurk Experiment.
2020
Crowdfunding is gaining popularity as a way of financing social sustainable initiatives. We performed a controlled economic experiment in MTurk by simulating a crowdfunding platform and developed a theoretical model that rationalizes herding behavior. The experiment was designed to test and quantify the causal effects of revealing specific information to prospective backers: (i) the number of early contributors already financing the project and (ii) positive opinions of other backers versus those of experts. The results show that early contributions to the campaign and positive opinions of peers act as a reinforcing signal to potential backers and affect backers&rsquo