Search results for "Micro"

Cancer invasion ad metastasis: discovering new targets for diagnosis and therapeutics

2009

Cancer invasion and metastases are the hallmarks of malignant tumors and the cause of most cancer deaths. Molecular alterations that arise during cancer progression and that generate abnormal gene products, activated signal transduction pathways, and altered cancer cell-host stroma interactions can be considered targets of therapeutic drugs. Some of these drugs have already entered clinical trials, while others have shown promising results. In this review, we examine some of the successive steps of the metastatic process: cancer cell migration and invasion of the tumor microenvironment, angiogenesis and following extravasation focusing on the deranged signalling pathways underlying the mali…

Análisis de procesos inmunorreguladores en helmintiasis intestinales mediante el modelo Echinostoma caproni-ratón

2021

En la presente Tesis Doctoral se analizan diferentes parámetros que intervienen en las interacciones parásito-hospedador en helmintiasis intestinales mediante el empleo del modelo experimental Echinostoma caproni-ratón ICR. Para ello, se realizan diferentes estudios en los que se analiza y compara la respuesta inmunitaria en infecciones primarias, asociadas a susceptibilidad, y secundarias, asociadas a resistencia. Los resultados obtenidos demuestran que la susceptibilidad del ratón a la infección primaria con E. caproni se debe a su incapacidad de responder con producción de IL-25 lo cual determina que se genere una respuesta Th1 asociada a susceptibilidad. Asimismo, estos resultados ponen…

Aislamiento y caracterización de vesículas extracelulares en especies del género Candida

2017

Se ha desarrollado un método de obtención de exosomas, a partir de cultivos de levaduras del género Candida, que ha permitido obtener microvesículas de elevada homogeneidad y pureza. Mediante microscopía electrónica de transmisión y técnicas de detección de nanopartículas (NTA), se ha podido constatar la presencia de microvesículas de tamaños comprendidos entre 100 y 200 nm. La caracterización de proteínas llevada a cabo por técnicas de Western blot, ha puesto de manifiesto la elevada pureza de los exosomas obtenidos, no existiendo contaminación celular en las preparaciones, tal y como lo indican el resultado positivo frente a calnexina en el lisado celular y negativo en las vesículas extra…

Explicit proton transfer in classical molecular dynamics simulations.

2014

We present Hydrogen Dynamics (HYDYN), a method that allows explicit proton transfer in classical force field molecular dynamics simulations at thermodynamic equilibrium. HYDYN reproduces the characteristic properties of the excess proton in water, from the special pair dance, to the continuous fluctuation between the limiting Eigen and Zundel complexes, and the water reorientation beyond the first solvation layer. Advantages of HYDYN with respect to existing methods are computational efficiency, microscopic reversibility, and easy parameterization for any force field peerReviewed

Genome degeneration and adaptation in a nascent stage of symbiosis

2014

Symbiotic associations between animals and microbes are ubiquitous in nature, with an estimated 15% of all insect species harboring intracellular bacterial symbionts. Most bacterial symbionts share many genomic features including small genomes, nucleotide composition bias, high coding density, and a paucity of mobile DNA, consistent with long-term host association. In this study, we focus on the early stages of genome degeneration in a recently derived insect-bacterial mutualistic intracellular association. We present the complete genome sequence and annotation of Sitophilus oryzae primary endosymbiont (SOPE). We also present the finished genome sequence and annotation of strain HS, a close…

Metabolomics: An Emerging Approach to Understand Pathogenesis and to Assess Diagnosis and Response to Treatment in Spondyloarthritis

2022

Spondyloarthritis (SpA) is a group of rheumatic diseases whose pathogenesis relies on a complex interplay between genetic and environmental factors. Over the last several years, the importance of the alteration of the gut microbiota, known as dysbiosis, and the interaction of bacterial products with host immunity have been highlighted as intriguing key players in SpA development. The recent advent of the so called “-omics” sciences, that include metabolomics, opened the way to a new approach to SpA through a deeper characterisation of the pathogenetic mechanisms behind the disease. In addition, metabolomics can reveal potential new biomarkers to diagnose and monitor SpA patients. The aim of…

Burst analysis tool for developing neuronal networks exhibiting highly varying action potential dynamics

2012

In this paper we propose a firing statistics based neuronal network burst detection algorithm for neuronal networks exhibiting highly variable action potential dynamics. Electrical activity of neuronal networks is generally analyzed by the occurrences of spikes and bursts both in time and space. Commonly accepted analysis tools employ burst detection algorithms based on predefined criteria. However, maturing neuronal networks, such as those originating from human embryonic stem cells (hESCs), exhibit highly variable network structure and time-varying dynamics. To explore the developing burst/spike activities of such networks, we propose a burst detection algorithm which utilizes the firing …

Halogen Bonds in 2,5-Dihalopyridine-Copper(I) Halide Coordination Polymers

2019

Two series of 2,5-dihalopyridine-Cu(I)A (A = I, Br) complexes based on 2-X-5-iodopyridine and 2-X-5-bromopyridine (X = F, Cl, Br and I) are characterized by using single-crystal X-ray diffraction analysis to examine the nature of C2&minus

Antibiofilm activity of a novel pyrrolomycin against staphylococcal biofilms of veterinary interest

2011

Microsoft Dynamics Ax 2012 ātrdarbības uzlabošana

2015

Microsoft Dynamics Ax 2012 ir jaunākais produkts no Microsoft Dynamics Ax produktu saimes. Lai jaunie un esoši klienti būtu apmierināti sistēmas, ir jānodrošina ātra un stabila tās darbība. Bakalaura darba mērķis ir Microsoft Dynamics Ax 2012 ātrdarbības analīze un uzlabošana. Darba teorētiskajā daļā tiek apskatīta Microsoft Dynamics Ax 2012 sistēmas uzbūve un ātrdarbības testēšanas rīki. Darba praktiskajā daļā tiek veikti sistēmas ātrdarbības testi, un veiktas izmaiņas sistēmas konfigurācijā un programmas kodā. Darba rezultātā tika panākts sistēmas ātrdarbības uzlabojums.