Search results for "Microbio"
showing 10 items of 8741 documents
Complete Genome Sequence of a New Ruminococcaceae Bacterium Isolated from Anaerobic Biomass Hydrolysis
2018
ABSTRACT A new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratory-scale leach bed biogas system. The draft annotated genome sequence presented in this study and 16S rRNA gene sequence analysis indicated the affiliation of HV4-5-B5C with the family Ruminococcaceae outside recently described genera.
Chemically Stressed Bacterial Communities in Anaerobic Digesters Exhibit Resilience and Ecological Flexibility
2020
Anaerobic digestion is a technology known for its potential in terms of methane production. During the digestion process, multiple metabolites of high value are synthesized. However, recent works have demonstrated the high robustness and resilience of the involved microbiomes; these attributes make it difficult to manipulate them in such a way that a specific metabolite is predominantly produced. Therefore, an exact understanding of the manipulability of anaerobic microbiomes may open up a treasure box for bio-based industries. In the present work, the effect of nalidixic acid, γ-aminobutyric acid (GABA), and sodium phosphate on the microbiome of digested sewage sludge from a water treatmen…
1,2-DICHLOROETHANE CONTAMINATED AQUIFER: MICROBIAL COMMUNITY RESPONSE TO BIOSTIMULATION IN MICROCOSM
2022
Ocena ścieków browarnianych w wybranych punktach linii technologicznej
2016
Źródłem ścieków browarnianych są: warzelnia, fermentownia, leżakownia, rozlewnia oraz miejsce mycia opakowań. W zależności od etapu produkcyjnego ścieki te cechują zróżnicowane parametry mikrobiologiczne i fizykochemiczne. Ze względu na dużą zawartość substancji organicznych mogą one stanowić pożywkę dla różnych grup mikroorganizmów. Celem badań była ocena mikrobiologiczna ścieków browarnianych w trzech różnych punktach linii technologicznej ze szczególnym uwzględnieniem obecności mikroorganizmów potencjalnie chorobotwórczych. Materiał badawczy stanowiły ścieki pobrane z komór zbierających ścieki 1 i 2 oraz kolektora przy wylocie do rzeki. Badania mikrobiologiczne obejmowały oznaczenia ogól…
Structural Insight into CVB3-VLP Non-Adjuvanted Vaccine
2020
Coxsackievirus B (CVB) enteroviruses are common pathogens that can cause acute and chronic myocarditis, dilated cardiomyopathy, aseptic meningitis, and they are hypothesized to be a causal factor in type 1 diabetes. The licensed enterovirus vaccines and those currently in clinical development are traditional inactivated or live attenuated vaccines. Even though these vaccines work well in the prevention of enterovirus diseases, new vaccine technologies, like virus-like particles (VLPs), can offer important advantages in the manufacturing and epitope engineering. We have previously produced VLPs for CVB3 and CVB1 in insect cells. Here, we describe the production of CVB3-VLPs with enhanced pro…
Eelness: Transcriptomic analysis of Vibrio-induced immune response in European eel
2014
La malaltia és l’alteració de l’estat fisiològic d’un individu, dificultant les seves funcions vitals i fins i tot, comportant a la mort. Tots els éssers vius poden contraure malaltia, aquesta, és majoritàriament provocada per microorganismes patògens, incloent bacteris, virus, protozous i fongs. Al llarg de la infecció, la relació hoste-patògen és clau. Per una banda, l’hoste ha de comptar amb un sistema immunitari eficaç, capaç de combatre l’infecció, mantenint les funcions vitals i per tant, evitant la mort. Per l’altre banda, el patogen desenvolupa una sèrie de factors per tal de aconseguir una invasió exitosa i per tant, provocant infecció. L’objectiu principal d’aquest tesi és l’estud…
Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.
1998
ABSTRACT In order to determine the specificities of PCR-based assays used for detecting Cryptosporidium parvum DNA, eight pairs of previously described PCR primers targeting six distinct regions of the Cryptosporidium genome were evaluated for the detection of C. parvum , the agent of human cryptosporidiosis, and C. muris , C. baileyi , and C. meleagridis , three Cryptosporidium species that infect birds or mammals but are not considered to be human pathogens. The four Cryptosporidium species were divided into two groups: C. parvum and C. meleagridis , which gave the same-sized fragments with all the reactions, and C. muris and C. baileyi , which gave positive results with primer pairs targ…
Increase in midgut microbiota load induces an apparent immune priming and increases tolerance to Bacillus thuringiensis
2010
Summary The insect immune system is comprised of both humoral and cellular components that are mobilized in response to parasitic or pathogenic infections. Activation of the immune response implies a consid- erable expenditure of energy and that is why insects rely on inducible pathways that are activated after coming into contact with the pathogenic agent. Known as immune priming, insects can prolong the activation of the immune response and transmit their immune status to the next generation. Starting from a laboratory colony of the lepidopteran Spodoptera exigua and using the lytic zone assay as a measure of the immune status, we selected for a sub-colony with high levels of immune activ…
Biofilm Related to Animal Health, Zoonosis and Food Transmitted Diseases: Alternative Targets for Antimicrobial Strategy?
2012
The biofilm is a complex community of microbial cells that are associated with a surface and enclosed in a self-produced biopolymer matrix. Such structured community is a response of bacteria to a signal system named quorum sensing (QS) constituted by inter-cell communication through the production of small molecules called autoinducers (AIs) that trigger a cascade of events in gene expression. When the concentration of the AIs molecules reach a certain threshold, the bacterial population acts as a single organism, collectively expressing virulence or biofilm forming genes. Gram-positive bacteria use modified oligopeptides as QS effectors molecules.
Okadaic Acid, an Apoptogenic Toxin for Symbiotic/Parasitic Annelids in the Demosponge Suberites domuncula
2006
ABSTRACT The role of okadaic acid (OA) in the defense system of the marine demosponge Suberites domuncula against symbiotic/parasitic annelids was examined. Bacteria within the mesohyl produced okadaic acid at concentrations between 32 ng/g and 58 ng/g of tissue (wet weight). By immunocytochemical methods and by use of antibodies against OA, we showed that the toxin was intracellularly stored in vesicles. Western blotting experiments demonstrated that OA also existed bound to a protein with a molecular weight of 35,000 which was tentatively identified as a galectin (by application of antigalectin antibodies). Annelids that are found in S. domuncula undergo apoptotic cell death. OA is one ca…