Search results for "Microbiología"
showing 10 items of 115 documents
Bases moleculares de la señalización mediada por dUTPasas triméricas de bacteriófagos
2021
Tesis doctoral, 328 p. Figuras y tablas.
dIvergEnt: How IgE Axis Contributes to the Continuum of Allergic Asthma and Anti-IgE Therapies
2017
[EN] Asthma is an airway disease characterised by chronic inflammation with intermittent or permanent symptoms including wheezing, shortness of breath, chest tightness, and cough, which vary in terms of their occurrence, frequency, and intensity. The most common associated feature in the airways of patients with asthma is airway inflammation. In recent decades, efforts have been made to characterise the heterogeneous clinical nature of asthma. The interest in improving the definitions of asthma phenotypes and endotypes is growing, although these classifications do not always correlate with prognosis nor are always appropriate therapeutic approaches. Attempts have been made to identify the m…
Estudio del compostaje como técnica para la eliminación de contaminantes emergentes
2017
Desde hace unos años está surgiendo una nueva clase de contaminantes químicos utilizados por el hombre con distintas finalidades y denominados contaminantes emergentes, entre los cuales se incluyen los productos farmacéuticos. Todos estos compuestos se encuentran en las aguas residuales, y como las estaciones de depuración de aguas residuales no están acondicionadas para eliminarlos, acaban liberándose en el medio ambiente. La mayor acumulación de estos compuestos se produce en los lodos que son utilizados en agricultura y compostaje. De hecho, se han encontrado fármacos de uso humano y fungicidas de aplicación agrícola en aguas superficiales, suelos, biosólidos e incluso en agua potable. E…
Solving gap metabolites and blocked reactions in genome-scale models: application to the metabolic network of Blattabacterium cuenoti
2013
Abstract Background Metabolic reconstruction is the computational-based process that aims to elucidate the network of metabolites interconnected through reactions catalyzed by activities assigned to one or more genes. Reconstructed models may contain inconsistencies that appear as gap metabolites and blocked reactions. Although automatic methods for solving this problem have been previously developed, there are many situations where manual curation is still needed. Results We introduce a general definition of gap metabolite that allows its detection in a straightforward manner. Moreover, a method for the detection of Unconnected Modules, defined as isolated sets of blocked reactions connect…
Degradación de aminas biógenas mediante sistemas microbianos. Identificación y caracterización de las enzimas responsables
2016
Las aminas son compuestos ampliamente presentes en la naturaleza que contienen uno o varios sustituyentes unidos a un átomo de nitrógeno. Son compuestos endógenos de las plantas que también pueden encontrarse en frutas frescas y verduras. Sin embargo, en los alimentos, las aminas se forman fundamentalmente en los procesos fermentativos, y durante el envejecimiento y la conservación. Las aminas biógenas (AB) se originan por la descarboxilación microbiana de los correspondientes aminoácidos precursores, por ello se denominan ‘biógenas’. Entre las AB, las más estudiadas son la histamina y la tiramina por sus implicaciones negativas para la salud. Están presentes en numerosos alimentos tales co…
Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
2011
We describe the gut bacterial diversity inhabiting two saprophagous syrphids and their breeding substrate (decayed tissues of the columnar cactus Isolatocereus dumortieri). We analyzed the gut microbiota of Copestylum latum (scooping larvae that feed on decayed cactus tissues) and Copestylum limbipenne (whose larvae can also feed on semiliquid tissues) using molecular techniques. DNA was extracted from larval guts and cactus tissues. The V1-V3 region of the 16S rRNA genes was amplified and sequenced. A total of 31079 sequences were obtained. The main findings are: C. limbipenne is dominated by several Enterobacteriaceae, including putative nitrogen-fixing genera and pectinolitic species and…
Comparative biochemistry of CO2 fixation and the evolution of autotrophy
1999
Carbon dioxide fixation is a polyphyletic trait that has evolved in widely separated prokaryotic branches. The three principal CO2-assimilation pathways are (i) the reductive pentose-phosphate cycle, i. e. the Calvin-Benson cycle; (ii) the reductive citric acid (or Arnon) cycle; and (iii) the net synthesis of acetyl-CoA from CO/CO2, or Wood pathway. Sequence analysis and the comparative biochemistry of these routes suggest that all of them were shaped to a considerable extent by the evolutionary recruitment of enzymes. Molecular phylogenetic trees show that the Calvin-Benson cycle was a relatively late development in the (eu)bacterial branch, suggesting that some form(s) of carbon assimilat…
Candida albicans fibrinogen binding mannoprotein: expression in clinical strains and immunogenicity in patients with candidiasis
1998
A 58 kDa cell wall-associated fibrinogen binding mannoprotein (mp58), previously characterized by our group in a Candida albicans laboratory strain (ATCC 26555), was found to be also present in the cell wall of clinical isolates of this fungus. Most strains examined appear to have functional mp58 species, as detected by their ability to bind fibrinogen. Western immunoblot analysis, with a monovalent polyclonal antibody generated against the mp58 species from strain ATCC 26555, revealed differences in recognition patterns depending on the strain tested and the culture conditions used. Serum samples from normal and Candida infected individuals were examined for the presence of antibodies agai…
The cell wall structure: developments in diagnosis and treatment of candidiasis
1998
Candidiasis are among the fungal infections the most difficult to diagnose and treat. Research focused on specific fungal components which are absent in the host, such as the cell wall has lead to a better understanding of Candida albicans pathogenicity and clinical impact. The cell wall is responsible for antigenic expression and primary interaction with the host. It is composed mainly of b-glucans, chitin and mannoproteins, which account for the rigidity of the wall and for the fungal morphology. Of these components, mannoproteins might carry a “morphogenetic code” which might modulate the molecular architecture of the cell wall. The features of specific cell wall proteins as part of buil…
Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture
2008
Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared …